The effect of resveratrol on the prevention of cisplatin ototoxicity

Abstract

One of the most important adverse effects of cisplatin, a chemotherapeutic agent which is widely used in the treatment of cancer patients, is hearing loss. This has primarily been associated with the loss of inner ear hairy and spiral ganglion cells due to oxidative stress. Resveratrol is known to be an antioxidant agent, which has the theoretical potential of preventing cisplatin-related ototoxicity. This experimental study was approved by Animal Ethics Committee of Inonu University (2008-20) and supported by Inonu University Scientific Research Projects Support Fund (2009-17). Thirty-four 3-month-old Wistar albino female rats weighing 210-270 g were used in the study. The animals were allocated into four groups: in cisplatin group (Group A), a single dose of 12 mg/kg cisplatin was administered intraperitoneally to 10 rats; in cisplatin + resveratrol group (Group B), a single dose of 12 mg/kg cisplatin and 10 mg/kg resveratrol were administered intraperitoneally for 5 days to 10 rats; in resveratrol group (Group C), 10 mg/kg resveratrol was administered intraperitoneally for 5 days to seven rats and in control group (Group D), resveratrol solvent (5% alcohol-95% physiological saline) was administered intraperitoneally for 5 days to seven rats. Resveratrol administration has begun 1 day before cisplatin administration in the group treated with cisplatin and resveratrol combination. Distortion product otoacoustic emission (DPOAE) (Grason Stadler, Madison, USA) measurements were performed in the same ear of all rats (right ear) under general anesthesia at baseline, 1st and 5th days after drug administration. Statistically significant distortion product amplitude reductions were found in the cisplatin group at 1,418, 2,003, 3,363, 5,660, 8,003 and 9,515 Hz frequencies. Whereas in the cisplatin + resveratrol group, statistically significant difference was found between 1st and 5th day measurements only at 3,996 Hz frequency. No significant differences were noted between the measurements either in the resveratrol or in the control groups. According to these results, cisplatin-related ototoxicity has been greatly prevented by resveratrol use.

Chemoprotective Effect of Noni (Morinda Citrifolia L.) Fruit Juice Against Cisplatin-Induced Nephrotoxicity

“…Co-administration of plant extracts with CIS can prevent/minimize the toxicity [50,51]. Moreover, the decline of SOD, CAT and GSH activities was revealed after CIS (5.0 mg/kg b. wt.…”

Does Resveratrol Have a Protective Effect on Tympanosclerosis Formation in Experimentally Induced Otitis Media?

“…They showed that resveratrol significantly reduced the formation of reactive oxygen species (ROS) and inhibited cyclooxygenase-2 expression following noise exposure [18] . Resveratrol was also demonstrated to have a protective effect against cisplatin ototoxicity [14][15][16] . In those studies, the protective effects of resveratrol were thought to be due to its potent antioxidant properties.…”

“…Resveratrol has anti-inflammatory, antioxidant, anticarcinogenic, and anti-ageing properties [12,13] . Although the protective effects of resveratrol against cisplatin-induced ototoxicity and noise-induced hearing loss were reported in recent studies [14][15][16][17][18] , there have been no published studies on the possible role of resveratrol in the prevention of TS.…”

The Protective Role of Molsidomine on the Cisplatin-Induced Ototoxicity

“…The rats were randomly assigned to 4 groups (n = 32) as follows: (1) The dosage of cisplatin and MOL were chosen depend on the previous dose-response studies that have been reported to cause ototoxicity and marked antioxidative and anti-inflammatory effects in rats respectively [6,12].…”

“…Many antioxidant agents have been studied to prevent CIO [6][7][8][9][10]. MOL is a prodrug, belonging to the class of sydnonimines, has been used widely as an antianginal agent as a vasodilator.…”