The effect of RGD peptide-conjugated magnetite cationic liposomes on cell growth and cell sheet harvesting

Ito, Akira; Ino, Kosuke; Kobayashi, Takeshi; Honda, Hiroyuki

doi:10.1016/j.biomaterials.2005.03.039

Cited by 185 publications

(114 citation statements)

References 26 publications

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“…Since previous reports performed cellular association and uptake studies of liposomes at a liposome concentration of 10-40 µg lipid/mL, 13,29,30) we used 20 µg lipid/mL of magnetic liposomes (as approximately 120 ng/mL of incorporated magnetic nanoparticles) for cytotoxicity and cellular association and uptake study. Prior to evaluating the cellular association of the magnetic liposomes, we assessed the cytotoxic effect of magnetic liposomes on Caco-2 cells.…”

Section: Effect Of Particle Size and Surface Charge Of Magnetic Liposmentioning

confidence: 99%

Influence of Physicochemical Properties and PEG Modification of Magnetic Liposomes on Their Interaction with Intestinal Epithelial Caco-2 Cells

Kono

Jinzai

Kotera

et al. 2017

Biological & Pharmaceutical Bulletin

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The present study aimed to investigate the effect of particle size (100, 500 nm), surface charge (cationic, neutral and anionic) and polyethylene glycol (PEG) modification of magnetic liposomes on their interaction with the human intestinal epithelial cell line, Caco-2. The cellular associated amount of all the magnetic liposomes was significantly increased by the presence of a magnetic field. The highest association and internalization into Caco-2 cells was observed with magnetic cationic liposomes. Moreover, small magnetic liposomes were more efficiently associated and taken up into the cells, than large ones. In contrast, PEG modification significantly attenuated the enhancing effect of the magnetic field on the cellular association of magnetic liposomes. We also found that magnetic cationic liposomes had the highest retention properties to Caco-2 cells. Moreover, the retention of large magnetic liposomes to the cells was much longer than that of small ones. In addition, magnetic cationic and neutral liposomes had relatively high stability in Caco-2 cells, whereas magnetic anionic liposomes rapidly degraded. These results indicate that the physicochemical properties and PEG modification of magnetic liposomes greatly influences their intestinal epithelial transport.

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Section: Effect Of Particle Size and Surface Charge Of Magnetic Liposmentioning

confidence: 99%

Influence of Physicochemical Properties and PEG Modification of Magnetic Liposomes on Their Interaction with Intestinal Epithelial Caco-2 Cells

Kono

Jinzai

Kotera

et al. 2017

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

show abstract

“…18,19 Array patterns of microparticles or cells have been utilized in the design of biosensors, as scaffolds for patterning proteins, and in cell cultivation. In recent years, various techniques for manipulating bioparticles on chips have been developed using hydrodynamics, 1,73 magnetic forces, [74][75][76][77][78][79][80] and DEP. 16,17,81-85 DEP was first described by Pohl as the motion of dielectric particles under the influence of a non-uniform electric filed.…”

Section: Dep-based Devices For Bioanalysismentioning

confidence: 99%

Microchemistry- and MEMS-based Integrated Electrochemical Devices for Bioassay Applications

Ino

2015

Electrochemistry

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Here, we present an overview of our recent research progress in development of electrochemical devices/systems for bioassays. These devices/systems are based on microchemistry and micro-electromechanical systems (MEMS) for sophisticated analytical and electrochemical measurements. In particular, we exploit the unique chemical reactions occurring in micrometer-size spaces and/or involving micrometer-size structures for bioassays, including cell analyses. This paper addresses five topics: probe-, chip-, large-scale-integration chip-, and dielectrophoresisbased devices, and electrodeposition of hydrogels for bioassays.

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“…[122] When the temperature is lowered, the hydrophobicity of the hydrogel is altered and cells are detached. Other alternative detachment methods rely on detachment cues based on magnetism, [123] ultra-sound [124] and electronic signals. Electroactive self-assembled monolayers (SAMs) can be synthesized with the RGD peptide to promote cell adhesion.…”

Section: Cell Detachmentmentioning

confidence: 99%

Electronic Control of Cell Cultures Using Conjugated Polymer Surfaces

Persson¹

2014

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In the field of bioelectronics various electronic materials and devices are used in combination with biological systems in order to create novel applications within cell biology and medicine. A famous example of a successful bioelectronics application is the pacemaker. Metals are the most common electrical conductors, whereas polymers are generally considered being insulators. However, in the late 1970s it was shown that a special class of polymers with conjugated double bonds, could in fact, after some chemical modifications, conduct electricity. This was the start of the research field known as conducting polymers, and then later on organic electronics, a research area that has grown rapidly during the last decades. Conjugated polymers are also suitable to interact and interface with cells and tissues, as they are generally soft, flexible and biocompatible. In addition, their chemical properties can be tailor-made through synthesis to fit biological requirements and functions. During the last years applications using organic bioelectronics have become numerous. This thesis describes applications based on different conjugated polymers aiming to stimulate and control cell cultures. When culturing cells it is of interest to be able to control events such as adhesion, spreading, proliferation, differentiation and detachment. First, the impact of different polymer compositions and redox states on the adhesion of bacteria and subsequent biofilm formation was investigated. Similar polymer electrodes were also used to steer differentiation of neural stem cells, through changes in the surface exposure of a relevant biomolecule. Controlled delivery of molecules was achieved by coating nanoporous membranes with polymers that swell and contract when changing the redox state. Finally, electronic control over cell detachment using a water-soluble polymer was achieved. When applying a positive potential to this polymer, it swells, cracks and finally detaches, taking the cells that was cultured on top along with it. Together, the work and results presented in this thesis demonstrate a versatile conjugated polymer technology to achieve electronic control of the different growth stages of cell cultures as well as cellular functions. POPULÄRVETENSKAPLIG SAMMANFATTNINGOlika plaster finns numera överallt omkring oss och utgör ett av de absolut vanligaste materialen i vår vardag. Även elektronik har blivit en naturlig del av vår tillvaro och finns i en mängd olika produkter. Inom medicinsk teknik kommer allt fler applikationer som innefattar elektronik, ett exempel är pacemakern. Arbetet som ligger till grund för denna avhandling strävar efter att kombinera plaster och elektronik för tillämpningar inom medicin och cellbiologi.Plaster består till största delen av polymerer samt olika tillsatser för att få ett material med önskade egenskaper. Polymerer är i sin tur uppbyggda av långa kedjor av identiska molekylära byggstenar, så kallade monomerer. Monomerens kemi och struktur bestämmer även egenskaperna hos polymeren. Med hjä...

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The effect of RGD peptide-conjugated magnetite cationic liposomes on cell growth and cell sheet harvesting

Cited by 185 publications

References 26 publications

Influence of Physicochemical Properties and PEG Modification of Magnetic Liposomes on Their Interaction with Intestinal Epithelial Caco-2 Cells

Influence of Physicochemical Properties and PEG Modification of Magnetic Liposomes on Their Interaction with Intestinal Epithelial Caco-2 Cells

Microchemistry- and MEMS-based Integrated Electrochemical Devices for Bioassay Applications

Electronic Control of Cell Cultures Using Conjugated Polymer Surfaces

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