Two experiments determined the effect of dietary riboflavin supplementation on egg yolk and albumen riboflavin concentrations, egg production, egg weight, shell thickness, hen weight, hatchability, incidence of clubbed down, and incidence of hemorrhagic embryos. In the first experiment, hens were fed rations containing 1.55, 2.20, 4.40, and 8.80 mg of riboflavin/kg of diet for 27 wk. Significant (P < .05) depressions in both yolk and albumen riboflavin concentrations were noted at the two lower riboflavin levels after 1 wk. Egg production, egg weight, hatchability, and hen weight were all significantly depressed by the two lower riboflavin levels later in the experiment when compared with the two higher levels. Results indicate that egg riboflavin concentrations are related to important production parameters that may be used to predict future dietary riboflavin inadequacies. In the second experiment, hens were fed either an unsupplemented diet or a riboflavin-adequate diet. Measurements of egg albumen riboflavin content, egg production, hatchability, and embryo abnormalities were made twice each week. Results showed depressed albumen riboflavin concentrations and hatchability and increased incidence of hemorrhagic embryos and clubbed down without changes in egg production during the 4- to 7-day period following feeding of the unsupplemented diet. These results show that low albumen riboflavin content immediately affect hatchability and embryonic development. The estimated minimum critical albumen riboflavin concentrations needed to support maximum reproductive function are between 1.9 and 2.9 micrograms of riboflavin/g of egg albumen. These critical values might be used to evaluate riboflavin status of laying and breeding flocks.