The effect of roflumilast and ibuprofen on testicular ischemia reperfusion injury: An experimental study

Özgür, Berat Cem

doi:10.14744/tjtes.2021.01361

Cited by 3 publications

(7 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…MDA content was assessed through a spectrophotometric method based on the color change that occurs when thiobarbituric acid reacts with MDA, as previously described 12 . Similarly, MPO activity was also evaluated spectrophotometrically, as mentioned before 6,13 . The method proposed by Paglia et al, was utilized to measure the glutathione peroxidase (GSH-Px) activity, which checks the enzyme's capacity to aid the conversion of reduced glutathione (GSH) into oxidized glutathione (GSSG) in the presence of hydrogen peroxide 4,14 .…”

Section: Biochemical Evaluationmentioning

confidence: 99%

“…The principal factors contributing to the damage of testicular parenchymal tissue include elevated reactive oxygen species (ROS) levels, increased calcium levels within mitochondria, and cellular apoptosis. In an effort to limit the negative impacts of ischemia-reperfusion injury, a variety of medical treatments, used in tandem with manual or surgical detorsion, are currently under investigation [4][5][6] .…”

Section: Introductionmentioning

confidence: 99%

“…Various parts of the plant, including aerial parts, flowers, and fruits, are harnessed for medicinal uses due to their anthelmintic, antispasmodic, and anxiolytic properties. Passionflower is employed as a treatment for a variety of conditions, ranging from burns and diarrhea to painful menstruation, neurotic disorders, and insomnia, among others 6,7 . Soumya et al's study first revealed that passionflower extract juice could mitigate myocardial infarction, partially through oxidative stress inhibition 8 .…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Efectos protectores de Passiflora incarnata en la lesión por isquemia-reperfusión en la torsión testicular: un estudio experimental en un modelo de rata

AzizoÓlu,

Arslan,

Gökalp-Ózkorkmaz

et al. 2024

CIRU

View full text Add to dashboard Cite

Objective:The current study aimed to explore the potential protective effect of Passiflora Incarnata L., (PI) in treating IR injury after testicular torsion in rats. Materials and methods: This research investigated the impact of PI on IR damage in male Wistar albino rats. Animals were divided to three groups: group 1 (sham), group 2 (IR), and group 3 (IR+PI). Results: The malondialdehyde (MDA), myeloperoxidase (MPO) and glutathione (GSH) levels did not significantly differ across the groups (p = 0.830, p = 0.153 and p=0.140, respectively). However, Group 3 demonstrated a superior total antioxidant status (TAS) value compared to Group 2 (p = 0.020). Concurrently, Group 3 presented a significantly diminished mean total oxidant status (TOS) relative to Group 2 (p = 0.009). Furthermore, Group 3 showed a markedly improved Johnsen score relative to Group 2 (p < 0.01). IR caused cell degeneration, apoptosis, and fibrosis in testicular tissues. PI treatment, however, mitigated these effects, preserved seminiferous tubule integrity and promoted regular spermatogenesis. Furthermore, it reduced expression of tumor necrosis factor-alpha (TNF-α), Bax, and Annexin V, signifying diminished inflammation and apoptosis, thereby supporting cell survival (p < 0.01, p < 0.01, p < 0.01, respectively). Conclusions: This study revealed that PI significantly reduces oxidative stress and testicular damage, potentially benefiting therapies for IR injuries.

show abstract

Section: Biochemical Evaluationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Efectos protectores de Passiflora incarnata en la lesión por isquemia-reperfusión en la torsión testicular: un estudio experimental en un modelo de rata

AzizoÓlu,

Arslan,

Gökalp-Ózkorkmaz

et al. 2024

CIRU

View full text Add to dashboard Cite

show abstract

“…[4] The occurrence of ovarian torsion is often linked to benign EXPERIMENTAL STUDY ovarian tumors and cysts, specifically dermoid cysts. [5,6] Passiflora incarnata L., (PI), commonly known as the passionflower, is a perennial plant that can grow up to 10 meters tall and produces egg-shaped edible fruits. These fruits are low in calories (41-53 kcal/100 g) and rich in vitamins such as A, C, B1, and B2, as well as essential minerals like calcium, phosphorus, and iron.…”

Section: Introductionmentioning

confidence: 99%

“…The passionflower is utilized to treat a wide array of ailments, from burns and diarrhea to menstrual pain, neurotic conditions, and insomnia. [6,7] A study by Soumya et al [8] first revealed that extracts of passionflower juice might help reduce heart attack damage, partly by inhibiting oxidative stress. Moreover, it has been found effective in managing morphine addiction and might be beneficial for conditions like convulsions or neuralgia.…”

Section: Introductionmentioning

confidence: 99%

Protective effects of passiflora incarnata on ovarian ischemia/reperfusion damage in rats with ovarian torsion

Arslan

2023

Ulus Travma Acil Cerrahi Derg

View full text Add to dashboard Cite

BACKGROUND: This study aimed to investigate whether Passiflora Incarnata (PI) has a protective effect against ischemia-reperfusion (IR)-induced oxidative and inflammatory ovarian damage. METHODS:The effects of PI on ovarian ischemia-reperfusion injury were investigated in female Wistar albino rats. The animals were randomly divided into three groups: Group 1 (sham), Group 2 (IR), and Group 3 (IR+PI). RESULTS:The mean levels of Malondialdehyde (MDA), Myeloperoxidase (MPO), and Total Oxidant Status (TOS) were higher in the IR group (p=0.025, p<0.001, and p=0.016, respectively). The Total Antioxidant Status (TAS) levels were lower in the IR group (p=0.005). Immunostaining revealed significant differences across the groups for Tumor necrosis factor-alpha (TNF-α): 13.84%, 49.51%, and 22.51% for Groups 1, 2, and 3, respectively (p<0.01). Bax: 10.53%, 46.74%, and 26.46% for Groups 1, 2, and 3, respectively (p<0.01). Annexin V: 12.24%, 44.86%, and 23.28% for Groups 1, 2, and 3, respectively (p<0.01). The mean scores for hemorrhage, inflammation, follicular degeneration, and congestion showed significant variations among the groups, all registering p<0.001.CONCLUSION: Passiflora Incarnata exhibited antioxidant, anti-inflammatory, and anti-apoptotic properties, promoting cell survival, histologically protecting ovarian tissue, and ameliorating IR injury by reducing oxidative stress.

show abstract

Phosphodiesterase inhibitors and testicular torsion: A systematic review and meta‐analysis of animal studies

Oliveira,

Sampaio,

Junior

et al. 2024

Andrology

View full text Add to dashboard Cite

BackgroundTesticular torsion is a common urological emergency in children and teenagers that, in most cases, requires surgical exploration and detorsion. However, even after a successful intervention, a high number of patients still develop irreversible damage.ObjectivesTo investigate whether the administration of phosphodiesterase inhibitors (PDE) in animal models of testicular torsion can reduce damages caused by the ischemia–reperfusion syndrome.Materials and methodsA systematic search of the Medline, Web of Science, Embase, and Ovid databases for studies up to December 2023 was performed using PRISMA guidelines. A detailed search was conducted using the following key terms: “testicles,” “ischemia,” “reperfusion,” and “phosphodiesterase inhibitors.” There was no restriction regarding language or year of publication. We investigated spermatogenesis by the Johnsen's biopsy score (JTBS) and histological damage by the Cosentino's biopsy score (CBS). Malondialdehyde (MDA), nitric oxide (NO), superoxide dismutase (SOD), and glutathione peroxidase (GPx) testicular levels were also analyzed.ResultsInitially, 296 articles were identified. However, only 20 studies met the inclusion criteria. PDE inhibitors improved JTBS (MD = 2.53, CI = 1.94 to 3.13), CBS (MD = −1.11, CI = −1.25 to −0.97) and SOD (SMD = 3.27, CI = 1.59 to 4.95) outcomes. PDE did not improve MDA (SMD = −0.93, CI = −2.19 to 0.34), NO (SMD = −0.54, CI = −1.56 to 0.48), and GPx (SMD = 0.77, CI = −0.38 to 1.92) outcomes. A higher dose of PDE inhibitors only improved CBS outcome.Discussion and conclusionThis review indicates that the administration of PDE inhibitors in rats ameliorated the outcomes, representing a promising complementary strategy for spermatogenesis recovery following testicular torsion. Our review suggests that JTBS and CBS could be translated into future human studies, validating and extending these findings within the context of human physiopathology, while providing applicability of interventions in real clinical practice.

show abstract

The effect of roflumilast and ibuprofen on testicular ischemia reperfusion injury: An experimental study

Cited by 3 publications

References 31 publications

Efectos protectores de Passiflora incarnata en la lesión por isquemia-reperfusión en la torsión testicular: un estudio experimental en un modelo de rata

Efectos protectores de Passiflora incarnata en la lesión por isquemia-reperfusión en la torsión testicular: un estudio experimental en un modelo de rata

Protective effects of passiflora incarnata on ovarian ischemia/reperfusion damage in rats with ovarian torsion

Phosphodiesterase inhibitors and testicular torsion: A systematic review and meta‐analysis of animal studies

Contact Info

Product

Resources

About