The effect of rosmarinus officinalis and chemotherapeutic etoposide on glioblastoma (u87 mg) cell culture

Özdemir, Meryem Damla; Göktürk, Dilek

doi:10.5137/1019-5149.jtn.20401-17.3

Cited by 7 publications

(7 citation statements)

References 16 publications

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“…According to previous study it was observed that while increased quantities of Rosmarinus Officinalis infusion solution induced MEF cell proliferation, it inhibited the survival of GBM cells in three and five days (48). In this study the same effect of Rosmarinus Officinalis infusion solution was observed in one day.…”

Section: Discussionsupporting

confidence: 80%

“…It was sterilized by 22 µM pore sized filter. From our previous studies 1/75 (v/v) rosemary has already been determined as optimum for both MEF and GBM cells (48). It is the dose which has minimum cytotoxic effect on the MEF cells and maximum cytotoxic effect on the GBM cells.…”

Section: Preparation Of the Rosmarinus Officinalis Infusion And Extraction Analysismentioning

confidence: 99%

“…(36)(37)(38). Furthermore many studies have been reported that rosemary possesses antimicrobial, antioxidant, anti-inflammatory and anti-cancer properties and also it is effective on various cancer types including glioma (39)(40)(41)(42)(43)(44)(45)(46)(47)(48).…”

Section: Introductionmentioning

confidence: 99%

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The concurrent effect of acyclovir and rosemary on glioblastoma cell culture

Özdemir

Göktürk

2019

Cell Mol Biol (Noisy-le-grand)

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Human cytomegalovirus (HCMV) is a beta herpesvirus which large amount of people in world has interacted with. Recent studies indicated that CMV DNA is associated with several cancer types including "Glioblastoma (GBM)" which is the most common and aggressive type of primary brain cancer. In clinical studies it was shown that several antiviral medicines prolonged life span of glioblastoma patients. One of them is Acyclovir (ACV) which is a type of nucleoside analog, used to cure viral infections and might be a potential treatment supplement for Glioblastoma. In this study we aimed to investigate if ACV had cytotoxic effect on glioblastoma cell line U87 MG and also the effect of ACV on healthy cells. Furthermore it was aimed to search the effect of Rosmarinus Officinalis also known as rosemary which is an aromatic, perennial plant concurrent with ACV on glioblastoma and healthy cells.

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Section: Discussionsupporting

confidence: 80%

Section: Preparation Of the Rosmarinus Officinalis Infusion And Extraction Analysismentioning

confidence: 99%

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The concurrent effect of acyclovir and rosemary on glioblastoma cell culture

Özdemir

Göktürk

2019

Cell Mol Biol (Noisy-le-grand)

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“…Campthothecin Inhibits PP1 and PP2 at serine/threonine phosphatases and cell growth inhibition 50 [129,130] Cycloheximide Inhibits p38 activated protein kinase (MAKPK) a nd cell entry at G2/M phase 5 [131,132] Etoposide Down regulate polymerase II at S-G2 phases and prevents cell division 50 [133,134] Ofloxacin 4-Fluorquinolone inhibits polymerase at G2/M phase, and apoptosis 30 [135,136] Praziquantel Isoquinolone inhibits serine/threonine kinase (PI3-k) polymerases I and II 46 [137][138][139] Staurosporine Protein kinases inhibitor (A, C, CAM and PI3K) prevents cell growth at G1 check point 9 [140,141] *Each drug suspension (µg/100 µL) in 0.15M NaCl used in the cytotoxic in vitro assays. µg…”

Section: Polymerase Inhibitorsmentioning

confidence: 99%

The Trypanosoma cruzi kinetoplast DNA minicircle sequences transfer biomarker of the multidrug treatment of Chagas disease

Teixeira

Sousa

Gomes

et al. 2021

Preprint

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BackgroundThe Trypanosoma cruzi infection renders the transfer of the mitochondrion kinetoplast DNA minicircle sequences into the host’s genome. The Aves are refractory to the infection, but chicks hatched from the T. cruzi inoculated eggs integrate the DNA minicircle sequences into the germ line cells. Rabbits, mice and chickens with the minicircle sequences mutations develop the Chagas cardiomyopathy and the DNA transfer underpins the heart disease.MethodologyThe PCR with the specific primer sets revealed the Protist nuclear DNA and the kinetoplast DNA in the agarose gels bands probed with the radiolabel specific sequences from tissues of the T. cruzi-infected rabbits and of the mice. A target- primer TAIL-PCR amplification employing primer sets from the chickens, rabbits and mice, in combination with primer sets from the the T. cruzi kinetoplast minicircle sequences was used. This approach led us to disclose the integration sites of the kinetoplast DNA biomarker, then, used to monitor the effect of multidrug treatment of the T. cruzi infected mice.Principal findingsThe Southern hybridization, clone and sequence of the amplification products revealed the DNA minicircle sequences integrations sites in the LINE transposable elements. An array of inhibitors of eukaryote cells division was used to arrest the DNA transfer. It was shown that nine out of 12 inhibitors prevented the kinetoplast DNA integration into the macrophage genome. The multidrug treatment of the acutely T. cruzi-infected mice with Benznidazole, Azidothymidine and Ofloxacin lessened circa 2.5-fold the rate of the minicircle sequences integrations in the mouse genome and inhibited the rejection of the target heart cells.Conclusion and significanceThe T. cruzi mitochondrion kinetoplast minicircle sequences transfer driven pathogenesis of Chagas disease is an ancient Cross-Kingdom DNA phenomenon of evolution and, therefore, paradigm research with effective purposing inhibitors is needed.Authors summaryChagas disease is considered the main cause of human heart failure in the Western Hemisphere. The treatment of the clinically manifested Chagas heart disease is considered unsatisfactory. Perhaps the most important problem in the field of Chagas disease is determination of the pathogenesis of the target heart cells lysis. We showed the transfer of the T. cruzi kDNA minicircle sequences into the genome of rabbits and mice, and to Bird refractory to the infections. The inoculation of a few T. cruzi in the fertile chicken eggs renders the kDNA sequences integration in the stem cells. Interestingly, the chicks that hatched retain the kDNA and develop the Chagas-like cardiomyopathy indistinguishable to that in the rabbits and mice. This result prompted the multidrug treatment of the Chagas heart disease with inhibitors of the eukaryotic cells division. We showed that nine out of 12 inhibitors prevented the transfer of the kDNA mutations into the macrophage genome, and that the treatment of the acutely T. cruzi-infected mice with Benznidazole + Ofloxacin + Azidothymidine lowered circa 2.5-fold the rate of the mutations in the chromosomes. These findings translated to the pathology showing inhibition of the heart lesions in the treated T. cruzi-infected mice. We suggest purposing new inhibitors should be tested to overturning the Chagas heart disease.

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“…Moreover, RE by itself promoted the growth of Mouse Embryonic Fibroblast (MEF) cells, while reducing the negative impact of etoposide on these cells when employed simultaneously with etoposide. It can be presumed that RE protects healthy cells from the negative effects of etoposide, without reducing its action in GBM cells [130].…”

Section: Synergistic Action and Chemoadjuvant Effect Of Rosemarymentioning

confidence: 99%

Anticancer Activity of Rosmarinus officinalis L.: Mechanisms of Action and Therapeutic Potentials

et al. 2020

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Alternative treatments for neoplastic diseases with new drugs are necessary because the clinical effectiveness of chemotherapy is often reduced by collateral effects. Several natural substances of plant origin have been demonstrated to be successful in the prevention and treatment of numerous tumors. Rosmarinus officinalis L. is a herb that is cultivated in diverse areas of the world. There is increasing attention being directed towards the pharmaceutical capacities of rosemary, utilized for its anti-inflammatory, anti-infective or anticancer action. The antitumor effect of rosemary has been related to diverse mechanisms, such as the antioxidant effect, antiangiogenic properties, epigenetic actions, regulation of the immune response and anti-inflammatory response, modification of specific metabolic pathways, and increased expression of onco-suppressor genes. In this review, we aim to report the results of preclinical studies dealing with the anticancer effects of rosemary, the molecular mechanisms related to these actions, and the interactions between rosemary and anticancer drugs. The prospect of utilizing rosemary as an agent in the treatment of different neoplastic diseases is discussed. However, although the use of rosemary in the therapy of neoplasms constitutes a fascinating field of study, large and controlled studies must be conducted to definitively clarify the real impact of this substance in clinical practice.

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The effect of rosmarinus officinalis and chemotherapeutic etoposide on glioblastoma (u87 mg) cell culture

Abstract: Rosmarinus Officinalis offers hope for developing new cancer treatment strategies. However, further studies are needed to verify these results.

Cited by 7 publications

References 16 publications

The concurrent effect of acyclovir and rosemary on glioblastoma cell culture

The concurrent effect of acyclovir and rosemary on glioblastoma cell culture

The Trypanosoma cruzi kinetoplast DNA minicircle sequences transfer biomarker of the multidrug treatment of Chagas disease

Anticancer Activity of Rosmarinus officinalis L.: Mechanisms of Action and Therapeutic Potentials

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