The effect of salinity stress on the growth and lipid accumulation of Scenedesmus quadricauda FACHB-1297 under xylose mixotrophic cultivation

Қоректік орта микробалдырлардың клеткаларының тіршілік әрекеті кезіндегі зат алмасу процестерін, энергия алмасуын қамтамасыз ететін, сондай-ақ өсуге маңызды элементтерді қамтитын субстрат. Микробалдырлардың қоректік ортасын өзгерту арқылы, олардың клеткаларының өсу көрсеткіштерін және химиялық құрамын өзгертуге болады. Бұл жұмыста микробалдыр Scenedesmus obliquus sp. B7 штамының өсу ортасына, яғни Прата қоректік ортасына қосымша қосылған натрий хлордың (NaCl) 2,5 г/л және 15 г/л аралықтағы концентрацияларын қосып, бұл орталарда өскен Scenedesmus obliquus sp. B7 штамының өсу көрсеткіштері, биомасса құрамындағы пигменттер (хлорофилл а, b) мен липидтердің мөлшері зерттелді. Бұл нәтижелер бойынша, бақылаумен салыстырғанда Scenedesmus оbliquussp. B7 штамының өсу динамикасы мен бірқатар пигменттер құрамының жиналуына NaCl-дың 10 г/л оңтайлы болып табылса, ал керісінше липидтердің мөлшері NaCl-дың 15 г/л-де ең жоғары мөлшерде жиналғаны анықталды. Мұндағы, өсу тығыздығы бақылаумен салыстырғанда NaCl-дың 10 г/л –де 80% - ға өскен болса, хлорофилл а және b көрсеткіштері 2,2 және 3 есеге дейін жоғарлағаны белгіленді. Ал, тәжірибедегі бақылаумен және басқа нұсқалармен салыстырғанда NaCl-дың 15 г/л концентрациясындағы биомасса құрамында липидтердің мөлшері 2,52 есе жоғарлағаны анықталды. Бұл көрсеткіштерден микробалдыр S. obliquus sp. B7 штаммының бізбен алынған тұздың жоғарғы концентрациясында (10 г/л) клеткаларының өсімі мен бейімделгіштігінің жоғары екенін көрсетеді. Nutrient medium is a substrate that provides metabolic processes during vital activity of microalgae cells, energy metabolism, and elements essential for microalgae growth. It is possible to alter the growth indicators and chemical composition of microalgae cells by changing the components of nutrient medium. In these studies, growth indicators, chlorophyll (a, b) and total lipid content in the biomass of Scenedesmus obliquus sp. B7 strain grown on Prata medium with the addition of sodium chloride concentrations in the intervals of 2.5 g/l and 15 g/l were evaluated. According to these results, compared with the observation, it was found that 10 g/l NaCl was optimal for the growth dynamics of Scenedesmus obliquus sp. B7 strain and accumulation of some pigment compositions. Nevertheless, the optimum value for the highest total lipid content was 15 g/l NaCl. It was determined that the chlorophyll a and b indicators increased by 2.2 and 3 times, respectively, when the growth density was increased by 80% in 10 g/l NaCl compared to the control. In comparison with experimental observation and other variants, it was ascertained that the content of total lipids in biomass was 2.52 times higher when 15 g/l NaCl was added to the nutrient medium. It can be concluded from these indicators that the microalgae Scenedesmus obliquus sp. B7 strain shows a high level of growth and cell adaptability at a NaCl concentration of 10 g/l in the nutrient medium.

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“…Lipids were determined by the gravimetric method after extraction from the biomass with a chloroform-isopropanol mixture (volume 1:1) [16].…”

Section: Research Materials and Methodsmentioning

confidence: 99%

“…Липидтер хлороформ-изопропанол қоспасымен (1:1 көлемі бойынша) биомассадан алынғаннан кейін гравиметриялық әдіспен анықталды [16].…”

Section: зерттеу материалдары мен әдістеріunclassified

The Effect of Salt Concentration on the Growth and Productivity of Microalgae Strains Scenedesmus Obliquus Sp. B7

АХМЕТКЕРИМ¹,

КИРБАЕВА²,

Sadvakasova³

et al. 2023

Микробиология Және Вирусология

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“…In previous studies, various microalgal species, like Chlorella sorokiniana and Desmodesmus asymmetricus [ 12 ], Chlamydomonas reinhardtii [ 13 ], Dunaliella salina [ 14 ], and Scenedesmus quadricauda [ 15 ], have been reported for their enhancement of lipid production under conditions of salinity stress. However, the increase in salinity conditions reduces microalgal growth and biomass productivity.…”

Section: Introductionmentioning

confidence: 99%

Salinity-Induced Physiochemical Alterations to Enhance Lipid Content in Oleaginous Microalgae Scenedesmus sp. BHU1 via Two-Stage Cultivation for Biodiesel Feedstock

et al. 2023

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In the recent past, various microalgae have been considered a renewable energy source for biofuel production, and their amount and extent can be enhanced by applying certain types of stress including salinity. Although microalgae growing under salinity stress result in a higher lipid content, they simultaneously reduce in growth and biomass output. To resolve this issue, the physiochemical changes in microalgae Scenedesmus sp. BHU1 have been assessed through two-stage cultivation. In stage-I, the maximum carbohydrate and lipid contents (39.55 and 34.10%) were found at a 0.4 M NaCl concentration, while in stage-II, the maximum carbohydrate and lipid contents (42.16 and 38.10%) were obtained in the 8-day-old culture. However, under increased salinity, Scenedesmus sp. BHU1 exhibited a decrease in photosynthetic attributes, including Chl-a, Chl-b, Fv/Fm, Y(II), Y(NPQ), NPQ, qP, qL, qN, and ETRmax but increased Y(NO) and carotenoids content. Apart from physiological attributes, osmoprotectants, stress biomarkers, and nonenzymatic antioxidants were also studied to elucidate the role of reactive oxygen species (ROS) facilitated lipid synthesis. Furthermore, elemental and mineral ion analysis of microalgal biomass was performed to evaluate the biomass quality for biofuel and cell homeostasis. Based on fluorometry analysis, we found the maximum neutral lipids in the 8-day-old grown culture at stage-II in Scenedesmus sp. BHU1. Furthermore, the use of Fourier-transform infrared (FT-IR) and nuclear magnetic resonance (NMR) spectroscopy analyses confirmed the presence of higher levels of hydrocarbons and triacylglycerides (TAGs) composed of saturated fatty acids (SFAs) and monounsaturated fatty acids (MUFAs) in the 8-day-old culture. Therefore, Scenedesmus sp. BHU1 can be a promising microalga for potential biodiesel feedstock.

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Increase in polyunsaturated fatty acids and carotenoid accumulation in the microalga Golenkinia brevispicula (Chlorophyceae) by manipulating spectral irradiance and salinity

Rearte,

Celis‐Pla,

Abdala‐Díaz

et al. 2024

Biotech & Bioengineering

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Microalgal biotechnology offers a promising platform for the sustainable production of diverse renewable bioactive compounds. The key distinction from other microbial bioprocesses lies in the critical role that light plays in cultures, as it serves as a source of environmental information to control metabolic processes. Therefore, we can use these criteria to design a bioprocess that aims to stimulate the accumulation of target molecules by controlling light exposure. We study the effect on biochemical and photobiological responses of Golenkinia brevispicula FAUBA‐3 to the exposition of different spectral irradiances (specifically, high‐fluence PAR of narrow yellow spectrum complemented with low intensity of monochromatic radiations of red, blue, and UV‐A) under prestress and salinity stress conditions. High light (HL) intensity coupled to salinity stress affected the photosynthetic activity and photoprotection mechanisms as shown by maximal quantum yield (Fv/Fm) and non‐photochemical quenching (NPQmax) reduction, respectively. HL treatments combined with the proper dose of UV‐A radiation under salinity stress induced the highest carotenoid content (2.75 mg g dry weight [DW]−1) composed mainly of lutein and β‐carotene, and the highest lipid accumulation (35.3% DW) with the highest polyunsaturated fatty acid content (alpha‐linolenic acid (C18:3) and linoleic acid (C18:2)). Our study can guide the strategies for commercial indoor production of G. brevispicula for high‐value metabolites.

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The effect of salinity stress on the growth and lipid accumulation of Scenedesmus quadricauda FACHB-1297 under xylose mixotrophic cultivation

Cited by 10 publications

References 49 publications

The Effect of Salt Concentration on the Growth and Productivity of Microalgae Strains Scenedesmus Obliquus Sp. B7

The Effect of Salt Concentration on the Growth and Productivity of Microalgae Strains Scenedesmus Obliquus Sp. B7

Salinity-Induced Physiochemical Alterations to Enhance Lipid Content in Oleaginous Microalgae Scenedesmus sp. BHU1 via Two-Stage Cultivation for Biodiesel Feedstock

Increase in polyunsaturated fatty acids and carotenoid accumulation in the microalga Golenkinia brevispicula (Chlorophyceae) by manipulating spectral irradiance and salinity

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