The effect of seed presoaking with KNO3 on seed germination, proline, protein pattern, ß-Amylase and mineral compositions of two faba bean cultivars treated with NaCl

Abstract

Different salinization levels (0, 40, 80, 120 and 160mM NaCl) were used and both cultivars were presoaked in either water (Reference control) or potassium nitrate (3mM), then left for germination for five days. Plants transported to the field and further growth for 65 days from sowing. The final germination percentage the two broad bean cultivars of Vicia faba c were decreased by increasing salinity in the rooting medium. Seed presoaking in KNO 3 resulted in remarkable increase in final germination percentage especially in cultivar Sakha 3. Both cultivars accumulated proline in both different organs reached 3 folds higher than absolute control, Seed presoaking in KNO 3 had a significant increase in proline contents especially in shoots regardless the salinity levels used. Mineral composition the plants of the two broad bean cultivars showed various responses with increasing salinity. Seed presoaking in KNO 3 resulted in remarkable decrease in Na and increased K contents in tested plants. One band of β-amylase were detected under different concentrations of NaCl combined with KNO 3. The intensity of the bands varied between various treatments. Exposure of Sakha 3 and Nobaria 3 cultivars to different concentrations of NaCl salinity and those presoaked in KNO 3 (3mM) were produced marked changes in their protein pattern.

The germination niche of coastal dune species as related to their occurrence along a sea–inland gradient

“…However, previous studies showed that nutrient availability significantly enhances seedling growth rate, improving their establishment (Maun, 1994;Del Vecchio et al, 2013) by incrementing seedling biomass (Frosini et al, 2012), and counteracting the effect of sand burial (Gilbert et al, 2008). Similarly to nitrogen alone, the interaction of nitrogen and salinity also seems to be particularly important during the post-germination phase: nitrogen supply proved to alleviate the toxic effect of salinity in seedlings, enhancing the activity of antioxidant enzymes in several species (Zheng et al, 2008;Abd El-Baki et al, 2018;Ibrahim et al, 2018). Therefore, we could affirm that nutrients such as nitrogen could limit species survival in adults but seems to have a less important role in the germination of coastal species.…”

“…Specifically, KNO 3 has been proven to alleviate salt-induced stress in several species (Neid & Biesboer, 2005;Atia et al, 2009;Zehra et al, 2013) including Elymus farctus (El-Katony et al, 2015). Conversely, KNO 3 could not alleviate the effect of salinity in Silene mollissima aggregate (Murru et al, 2015) and in Vicia faba (Abd El-Baki et al, 2018). However, unlike salinity and temperature, which in general decrease linearly from the sea DEL VECCHIO Et aL.…”