The effect of selected organic solvents on intact human red cell membrane acetylcholinesterase in vitro

Korpela, M.; Tähti, Hanna

doi:10.1016/0041-008x(86)90120-1

Cited by 28 publications

(11 citation statements)

References 18 publications

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“…These results agree with previous findings with erythrocyte membrane (5)(6)(7). Organic solvents affect both erythrocyte membrane AChE, which is situated on the outer half of the membrane, and A'TPase, the location of which is mainly on the cytoplasmic side of the erythrocyte membrane (12).…”

Section: Discussionsupporting

confidence: 83%

“…In previous studies by my co-wo rkers and I, the interaction of organic solvents with integral proteins of erythrocyte membranes has been demonstrated in human s and rats at anesthetic and lower concentrations (5)(6)(7). In the present study I have investigated the chan ges in the activities of a cetylcholinesterase (AChE), total adenosinetriphosphatase (total ATPase), a nd ma gnesium-activat ed adenosinetriphosphatase (Mg 2 +--AT Pase) in rat syna ptoso me membranes expo sed to organic solvents und er in vitro conditions to evaluate further the mechani sm of the solvent-induced eNS depression on the cell membrane.…”

mentioning

confidence: 99%

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Inhibition of synaptosome membrane-bound integral enzymes by organic solvents.

Korpela¹

1989

Scand J Work Environ Health

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Section: Discussionsupporting

confidence: 83%

mentioning

confidence: 99%

Inhibition of synaptosome membrane-bound integral enzymes by organic solvents.

Korpela¹

1989

Scand J Work Environ Health

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“…In our previous studies we have found that both aromatic hydrocarbons and aliphatic chlorinated hydrocarbons inhibit erythrocyte and synaptosome integral enzymes dosedependently in in vitro conditions (Korpela unpublished results;Korpela & Tahti 1986. In the present study, both erythrocyte and synaptosomebound AChE, total ATPase and Mg2+-ATPase were inhibited.…”

Section: Discussionsupporting

confidence: 69%

The Effect of in Vitro and in Vivo Toluene Exposure on Rat Erythrocyte and Synaptosome Membrane Integral Enzymes

Korpela¹,

Tähti²

1988

Pharmacology & Toxicology

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The mechanism of the anaesthetic effect of toluene on the central nervous system (CNS) was studied by using rat erythrocyte and synaptosome membranes as nerve cell models both in vitro and in vivo. The activities of the membrane-bound integral enzymes acetylcholinesterase (AChE), total adenosine triphosphatase (total ATPase) and magnesium-activated adenosine triphosphatase (Mg2+-ATPase) were determined. A short-term exposure to 2000 p.p.m. of toluene had an inhibitory effect on the enzyme activities studied. The degree of inhibition in erythrocyte membranes in vitro and in vivo, and in synaptosome membranes in vitro were in good correlation. In in vivo conditions, the synaptosome-bound enzymes were, however, significantly more inhibited by toluene, which indicates that membranes in vivo are even more vulnerable to the toxic effects of organic solvents than they are as isolated membranes in vitro. However, our results show that in vitro experiments can be used to predict the toxic nerve cell membrane effects of organic solvents. Toluene caused similar enzyme inhibitions both in neural cell membranes and in erythrocyte membranes. Thus, even peripheral non-excitable cell membranes, like erythrocytes, can be used as nerve cell membrane models in studies on the mechanism of the anaesthesia caused by solvents.

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“…The changes in the nerve cell membrane as a consequence of the anesthetic action of solvents are nonspecific, and we have also seen them in peripheral unexcitable cells (4). Thus, we consider the erythrocyte membrane to be a convenient membrane model, especially for in vitro studies of the mechanisms of anesthesia.…”

Section: Discussionmentioning

confidence: 99%

“…In our previous studies, we used the erythrocyte membrane-bound enzyme acetylcholine esterase (AChE) as a model in studying the effects of organic solvents on membrane proteins (4). We found that organic solvents inhibit the activity of AChE, which belongs to the integral proteins of the membrane and is situated mainly on the outer half of the lipid bilayer (8 In the present study, we investigated the effects of organic solvents on another membrane integral protein, adenosine triphosphatase (ATPase), the location of which differs from that of AChE, ATPase being mainly situated on the cytoplasmic side of the membrane (8).…”

mentioning

confidence: 99%

Effects of industrial organic solvents on human erythrocyte membrane adenosine triphosphatase activities in vitro.

Korpela¹,

Tähti²

1987

Scand J Work Environ Health

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CorrectionsSee 1988;14(1):0 for a correction.Key terms: adeno sine triphosphatase; alcohols; aliphatic chlorinated hydrocarbon; amendment and correction; aromatic hydrocarbon; erythrocyte membrane adeno sine triphosphatase; human erythrocyte membrane; human erythrocyte membrane adeno sine triphosphatase; human erythrocyte membrane adeno sine triphosphatase activity; in vitro; industrial organic solvent; membrane effect; organic solvent This article in PubMed: www.ncbi.nlm.nih.gov/pubmed/2963375

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The effect of selected organic solvents on intact human red cell membrane acetylcholinesterase in vitro

Cited by 28 publications

References 18 publications

Inhibition of synaptosome membrane-bound integral enzymes by organic solvents.

Inhibition of synaptosome membrane-bound integral enzymes by organic solvents.

The Effect of in Vitro and in Vivo Toluene Exposure on Rat Erythrocyte and Synaptosome Membrane Integral Enzymes

Effects of industrial organic solvents on human erythrocyte membrane adenosine triphosphatase activities in vitro.

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