The Effect Of Selenium Supplementation On The Expression Of Nuclear Factor Erythroid 2-Related Factor 2 (Nrf2) And Malondialdehyde (MDA) In Wistar Rats Fed An Atherogenic Diet

Abstract

Background: Selenium is a trace element essential for maintaining the body's redox balance. Insufficient or excessive selenium intake has been known to cause redox balance disorders and can induce endogenous antioxidants such as nuclear factor erythroid 2-related factor 2 (Nrf2). This study aimed to determine the effect of different selenium doses on Nrf2 expression and MDA levels in Wistar rats fed an atherogenic diet. Methods: Experimental study with Post Test Only Control Group Design were 30 male Wistar rats who were randomized into three groups and given treatment for six weeks. The control group was only assigned an atherogenic diet without adding selenium supplementation. In the treatment group, I was given additional supplementation of sodium selenite 0.0086 mg/100 g diet. The second treatment group was also assigned sodium selenite supplementation 0.1 mg/100 g diet. After treatment, Nrf2 expression and MDA levels. Results: The mean of Nrf2 expression in this study did not show a significant difference between the control group (29,73±5.87 ng/ml) and treatment group I (29.89±5.29 ng/ml), and treatment group II (28.42±4,36 ng/ml) with p 0,807. A similar result was also obtained for the mean level of MDA in the control group (2.17±0.49), treatment group I (2.68±0.40), and treatment group II (2 .46±0.75) with p 0,166. Conclusion: These findings concluded that administration of low selenium supplementation, in this study, was not different in increasing Nrf2 gene expression and decreasing MDA levels in male Wistar rats fed with an atherogenic diet than high selenium supplementation.