The Effect of Shaking, CO2 Concentration and Light Intensity on Biomass Growth of Green Microalgae Desmodesmus communis

Abstract

There are many factors that can affect microalgae growth. In this research, four different groups of experiments were set up in order to determine the influence of different mixing conditions, CO 2 concentration and light intensities on Desmodesmus communis growth. The range of CO 2 concentration in the air-CO 2 mixture was 0-16 v/v%, light intensities ranged between 100 µmol m-2 s-1 and 300 µmol m-2 s-1. The best biomass productivity and biomass yield of 0.54 g d-1 and 3.53 g l-1 respectively were achieved when mixing was provided by using shaker as well as gas bubbling with air-CO 2 mixture of 96:4 v/v% and light intensity of 300 µmol m-2 s-1 .

Influence of Light Intensity and Temperature on Cultivation of Microalgae Desmodesmus Communis in Flasks and Laboratory-Scale Stirred Tank Photobioreactor

“…As mentioned earlier, in order to gain the best results by cultivating microalgae in a photobioreactor, first, the optimal conditions in flasks should be achieved; therefore, in previous work [21] the basal operating conditions of environmental factors were determined. The previous studies have shown that the aeration by air-CO 2 mixture 96:4 v/v% which was continuously supplied at a flow rate of 0.05 vvm has no limitation effect on the algae growth rate when mixing (by shaker) is provided.…”

“…All experiments were run in duplicates. Schematic diagram of the experimental setup is shown in previous report [21].…”

“…In previous study [21], the effect of three different light intensities (100, 200, and 300 µmol m -2 s -1 ) on the algae growth was evaluated, and it was concluded that in this range the best is 300 µmol m -2 s -1 . In this study, the range of light intensities was extended (200, 300, 400, 500, and 600 µmol m -2 s -1 ) in order to determine the effect of even higher light supply (see Fig.…”

“…Therefore, series of experiments were run in laboratory bottles under different temperatures (21,23,25,27, and 29 °C) in order to determine the optimal temperature and the temperature influence on the algae growth rate and productivity for D. Communis cultivation. The results obtained are shown in Fig.…”

Potential Application of Microalgae in Animal Production

“…KR-1, which at 40°C is characterized by high growth kinetics and high culture density (Sung et al 1999). The described organisms require proper light intensity and regulation of the lightdark cycle for normal biochemical reactions connected with this process (Vanags et al 2015). The kinetic growth of microalgae is proportional to light intensity, up until reaching saturation threshold (Carvalho et al 2011).…”