The effect of shear stress on cardiac differentiation of mesenchymal stem cells

Izadpanah, Peyman; Golchin, Ali; Firuzyar, Tahereh; Najafi, Masoud; Jangjou, Ali; Hashemi, Sheida

doi:10.1007/s11033-022-07149-y

Cited by 6 publications

(3 citation statements)

References 41 publications

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“…With in-depth study of stem cell therapy, researchers gradually identified problems that have hindered further clinical applications. First, the efficiency of natural stem cell differentiation is very low in vitro, so researchers have attempted to improve the efficiency of BMSC differentiation into CMs [11][12][13]. At present, commonly used methods to induce differentiation in vitro include selection of appropriate inducers, contact co-culture, and other strategies [14].…”

Section: Discussionmentioning

confidence: 99%

Preparation of myocardial patches from DiI-labeled rat bone marrow mesenchymal stem cells and neonatal rat cardiomyocytes contact co-cultured on polycaprolactone film

Zhang¹,

Zhang²,

Zhang³

et al. 2022

Biomed. Mater.

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DiI-labeled BMSCs were contact co-cultured with CMs on PCL film to prepare myocardial patches. BMSCs were labeled with DiI dye. DiI-labeled BMSCs were co-cultured with CMs on PCL film in the experimental group, while CMs were replaced with the same amount of unlabeled BMSCs in the control group. After 24 h, cell growth was observed by light microscopy and cells were fixed for scanning electron microscopy. After 7 days of co-culture, cells were stained for immunofluorescence detection of myocardial markers cardiac troponin T (cTnT) and α-actin. Differentiation of BMSCs on PCL was observed by fluorescence microscopy. The efficiency of BMSC differentiation into CMs was analyzed by flow cytometry on the first and seventh days of co-culture. CMs were stained with calcein alone and contact co-cultured with DiI-labeled BMSCs on PCL film to observe intercellular dye transfer. Finally, cells were stained for immunofluorescence detection of connexin 43 (Cx43) expression and to observe the relationship between gap junctions and contact co-culture. After co-culture for 24 h, cells were observed to have attached to PCL by light microscopy. Upon appropriate excitation, DiI-labeled BMSCs exhibited red fluorescence, while unlabeled CMs did not. Scanning electron microscopy revealed a large number of cells on the PCL membrane and their cell state appeared normal. On the seventh day, some DiI-labeled BMSCs expressed cTnT and α-actin. Flow cytometry showed that the rate of stem cell differentiation in the experimental group was significantly higher than the control group on the seven day (20.12% > 3.49%, P < 0.05). From the second day of co-culture, immunofluorescence staining for Cx43 revealed green fluorescent puncta in some BMSCs; from the third day of co-culture, a portion of BMSCs exhibited green fluorescence in dye transfer tests. Contact co-culture of DiI-labeled BMSCs and CMs on PCL film successfully generated myocardial patches.

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Section: Discussionmentioning

confidence: 99%

Preparation of myocardial patches from DiI-labeled rat bone marrow mesenchymal stem cells and neonatal rat cardiomyocytes contact co-cultured on polycaprolactone film

Zhang¹,

Zhang²,

Zhang³

et al. 2022

Biomed. Mater.

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“…The results of real-time PCR and ICC analysis suggested that the expression of cardiac genes was significantly improved in the microfluidic cell culture that used both 5-Azacytidine (5-Aza) and a shear stress of 1 Pascal compared to that in the static 2D environment. Interestingly, no change was observed in the 5-Aza-only or shear stress-only groups [ 134 ]. A later study by Ravishankar et al assessed the role of cyclic mechanical strain on endothelial progenitor cells’ (EPCs) differentiation.…”

Section: Challenges and Future Perspectivesmentioning

confidence: 99%

Exploring Electrospun Scaffold Innovations in Cardiovascular Therapy: A Review of Electrospinning in Cardiovascular Disease

Broadwin,

Imarhia,

et al. 2024

Bioengineering

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Cardiovascular disease (CVD) remains the leading cause of mortality worldwide. In particular, patients who suffer from ischemic heart disease (IHD) that is not amenable to surgical or percutaneous revascularization techniques have limited treatment options. Furthermore, after revascularization is successfully implemented, there are a number of pathophysiological changes to the myocardium, including but not limited to ischemia-reperfusion injury, necrosis, altered inflammation, tissue remodeling, and dyskinetic wall motion. Electrospinning, a nanofiber scaffold fabrication technique, has recently emerged as an attractive option as a potential therapeutic platform for the treatment of cardiovascular disease. Electrospun scaffolds made of biocompatible materials have the ability to mimic the native extracellular matrix and are compatible with drug delivery. These inherent properties, combined with ease of customization and a low cost of production, have made electrospun scaffolds an active area of research for the treatment of cardiovascular disease. In this review, we aim to discuss the current state of electrospinning from the fundamentals of scaffold creation to the current role of electrospun materials as both bioengineered extracellular matrices and drug delivery vehicles in the treatment of CVD, with a special emphasis on the potential clinical applications in myocardial ischemia.

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“…This combination has driven improvements in the clinical approach to treating bone defects. High levels of cardiac-related gene expression were not observed in either of the 5-azacytidiner (5-Aza) or shear stress groups, whereas BMSCs cultured with 5-Aza in concert with shear stress showed significantly increased cardiac-related gene expression [ 52 ], which is expected to promote cardiac differentiation of stem cells. In addition to shear stress combining with biochemical conditions to regulate stem cell behaviour, shear stress can also work in concert with other physical conditions.…”

Section: Mechanical Microenvironment For Stem Cell Growthmentioning

confidence: 99%

How the mechanical microenvironment of stem cell growth affects their differentiation: a review

Zhang

Wang

2022

Stem Cell Res Ther

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Stem cell differentiation is of great interest in medical research; however, specifically and effectively regulating stem cell differentiation is still a challenge. In addition to chemical factors, physical signals are an important component of the stem cell ecotone. The mechanical microenvironment of stem cells has a huge role in stem cell differentiation. Herein, we describe the knowledge accumulated to date on the mechanical environment in which stem cells exist, which consists of various factors, including the extracellular matrix and topology, substrate stiffness, shear stress, hydrostatic pressure, tension, and microgravity. We then detail the currently known signalling pathways that stem cells use to perceive the mechanical environment, including those involving nuclear factor-kB, the nicotinic acetylcholine receptor, the piezoelectric mechanosensitive ion channel, and hypoxia-inducible factor 1α. Using this information in clinical settings to treat diseases is the goal of this research, and we describe the progress that has been made. In this review, we examined the effects of mechanical factors in the stem cell growth microenvironment on stem cell differentiation, how mechanical signals are transmitted to and function within the cell, and the influence of mechanical factors on the use of stem cells in clinical applications.

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The effect of shear stress on cardiac differentiation of mesenchymal stem cells

Cited by 6 publications

References 41 publications

Preparation of myocardial patches from DiI-labeled rat bone marrow mesenchymal stem cells and neonatal rat cardiomyocytes contact co-cultured on polycaprolactone film

Preparation of myocardial patches from DiI-labeled rat bone marrow mesenchymal stem cells and neonatal rat cardiomyocytes contact co-cultured on polycaprolactone film

Exploring Electrospun Scaffold Innovations in Cardiovascular Therapy: A Review of Electrospinning in Cardiovascular Disease

How the mechanical microenvironment of stem cell growth affects their differentiation: a review

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