The effect of shore location on biomarker expression in wild Mytilus spp. and its comparison with long line cultivated mussels

Schmidt, Wiebke; O'Shea, Tracy; Quinn, Brian

doi:10.1016/j.marenvres.2012.07.002

Cited by 16 publications

(9 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[34] Chemical analyses Selection of suitable chemical concentrations for exposure is a crucial aspect of experimental design. In addition CF values were in the same range as those previously published for wild animals taken from the same mussel population.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

A proteomic evaluation of the effects of the pharmaceuticals diclofenac and gemfibrozil on marine mussels (Mytilus spp.): evidence for chronic sublethal effects on stress‐response proteins

Schmidt

Rainville

McEneff

et al. 2013

Drug Testing and Analysis

Self Cite

View full text Add to dashboard Cite

Human pharmaceuticals (e.g. the lipid regulator gemfibrozil and the non-steroidal anti-inflammatory drug diclofenac) are an emerging environmental threat in the aquatic environment. This study aimed to evaluate sublethal effects of these two commonly found pharmaceuticals on the protein profiles of marine mussels (Mytilus spp.). Mytilus spp. was exposed to environmentally relevant and elevated concentrations (1 and 1000 µg/l respectively) of both drugs for 14 days. In addition, mussels were maintained for seven days post treatment to examine the potential of blue mussels to recover from such an exposure. Differential protein expression signatures (PES) in the digestive gland of mussels were obtained using two-dimensional gel electrophoresis after 7, 14, and 21 days of exposure. Twelve spots were significantly increased or decreased by gemfibrozil and/or diclofenac, seven of which were successfully identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. These proteins were involved in energy metabolism, oxidative stress response, protein folding, and immune responses. Changes in the PES over time suggested that mussels were still experiencing oxidative stress for up to seven days post exposure. In addition, a suite of biomarkers comprising glutathione transferase, lipid peroxidation, and DNA damage were studied. An oxidative stress response was confirmed by biomarker responses. To our knowledge, this is the first investigation using proteomics to assess the potential effects of human pharmaceuticals on a non-target species in an environmentally-relevant model. The successful application of this proteomic approach supports its potential use in pollution biomonitoring and highlights its ability to aid in the discovery of new biomarkers.

show abstract

Section: Discussionmentioning

confidence: 99%

“…In addition CF values were in the same range as those previously published for wild animals taken from the same mussel population. [34] Chemical analyses…”

Section: Discussionmentioning

confidence: 99%

A proteomic evaluation of the effects of the pharmaceuticals diclofenac and gemfibrozil on marine mussels (Mytilus spp.): evidence for chronic sublethal effects on stress‐response proteins

Schmidt

Rainville

McEneff

et al. 2013

Drug Testing and Analysis

Self Cite

View full text Add to dashboard Cite

show abstract

“…The study is unique in its design as it completes the study from the same location performed by Nahrgang et al (2013) with continuous logging of environmental conditions over the entire year, description of the reproductive cycle of the local mussels, their monthly levels of the baseline biomarkers, and the validation of the findings of this location across a spatial sampling. In addition, measurement of the baseline biomarker levels in the mussels from upper and lower littoral zone underlines the importance of the tidal zone for future biomonitoring studies previously reported only in few papers (Izagirre et al, 2008;Letendre et al, 2009;Schmidt et al, 2012).…”

Section: Introductionmentioning

confidence: 85%

“…We hypothesized that the levels of biomarkers vary over the course of a year due to changes in biological function associated with the annual reproductive cycle (Schmidt et al, 2013;González-Fernández et al, 2016) and seasonality in environmental conditions (photoperiod, temperature, salinity and food supply) (Bebianno et al, 2007;Brenner et al, 2014). Moreover, differences in biomarkers related to the location of the mussels within the mussel bed were expected (Letendre et al, 2009;Schmidt et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Seasonal and spatial variations in biomarker baseline levels within Arctic populations of mussels (Mytilus spp.)

Storhaug

Nahrgang

Pedersen

et al. 2019

Science of The Total Environment

View full text Add to dashboard Cite

Climate change and pollution resulting from human activities in the Arctic require reliable monitoring systems and these are usually based on biomarkers in sentinel species.Mytilus spp. are used as sentinel species all around the world. The use of Mytilus spp. in environmental monitoring requires knowledge of their biology and natural variations in biomarkers. Seasonal variations in baseline levels of pollution biomarkers were studied over a year in the mussels from upper and lower littoral zone in Rakkfjorden, Norway, as they underwent their annual reproductive cycle. Spatial variations of these baseline biomarker levels were measured in five mussel populations within a 60-km radius from Rakkfjorden to investigate universality of the results from the specific population of Rakkfjorden at a regional scale. Seasonal variations in biomarker levels were revealed and seemed to be related to the reproductive state of the mussels and the tidal zone. The mussels appeared to be more sensitive to oxidative stress during gametogenesis in autumn and winter, when having lower lysosome membrane stability and lower baseline levels of antioxidant biomarkers. An increase in baseline levels of these biomarkers was reported during spawning in spring, however, it was not possible to reveal whether these changes were due to spawning, or to a higher metabolic activity in mussels in response to elevated water temperature and food abundance. The differences between the tidal zones reflected in reduced size of the mussels from the upper littoral zone, their late spawning in the season and increased baseline levels of antioxidant biomarkers during the coldest month, indicating a more challenging environment in the upper littoral zone. The spatial study indicated that the baseline biomarker levels measured in Rakkfjorden were no different from the levels measured in the mussels from five other sites and thus, are representative for all mussels on a regional scale.

show abstract

“…Bivalve (Vidal et al, 2002) Bivalve (Vidal et al, 2002) Bivalve (Schmidt et al, 2012 Crustacean (Naylor et al, 1989;Maltby et al, 2002) Crustacean (Maltby et al, 1990) Crustacean (McCahon et al, 1988;Pascoe et al, 1995;Fielding et al, 2003) Energy…”

Section: ) Lysosomal Biomarkersmentioning

confidence: 99%

Individual Biomarkers

Berthet¹

2015

Aquatic Ecotoxicology

View full text Add to dashboard Cite

The effect of shore location on biomarker expression in wild Mytilus spp. and its comparison with long line cultivated mussels

Cited by 16 publications

References 42 publications

A proteomic evaluation of the effects of the pharmaceuticals diclofenac and gemfibrozil on marine mussels (Mytilus spp.): evidence for chronic sublethal effects on stress‐response proteins

A proteomic evaluation of the effects of the pharmaceuticals diclofenac and gemfibrozil on marine mussels (Mytilus spp.): evidence for chronic sublethal effects on stress‐response proteins

Seasonal and spatial variations in biomarker baseline levels within Arctic populations of mussels (Mytilus spp.)

Individual Biomarkers

Contact Info

Product

Resources

About