The Effect of Silver Diamine Fluoride on Surface Characterization of Demineralized Dentin

Abstract

Purpose: The purpose of this study was to evaluate the effect of Silver Diamine Fluoride (SDF) application on Surface characterization of demineralized dentin using environmental scanning electron microscope (ESEM) Materials and Methods: A total of 40 teeth were used; the roots of each tooth were cut off at the CEJ. Each tooth was sectioned into 2 halves mesio-distally; resulting in 2 halves per each tooth, with total of 80 halves. The 80 halves were divided into 2 groups (n= 40 halves) according to the type of dentin (either demineralized or sound). Each group was subdivided into 2 subgroups (n= 20) according to SDF application (either with SDF or No SDF). The dentin Specimens (n=40 halves) were placed in artificial demineralizing solution for 72 hours. A small cotton pellet or brush was used to paint approximately one drop of SDF onto the middle dentine of each half for at least one minute then air-dry. After the lesions have been exposed to SDF for the determined period of time, the SDF was rinsed off for 30 seconds with distilled water. Each dentin surface was scanned under environmental scanning electron microscope to obtain images of dentin surface. Results: ESEM images revealed that on comparing the specimens of sound dentin to demineralized dentin upon SDF application, it was found that SDF was deposited on side walls of dentinal tubules in sound dentin while it was deposited inside the opened dentinal tubules in demineralized dentin. Conclusion: Upon application of Silver Diamine Fluoride, a deeper penetration of SDF into dentinal tubules of demineralized dentin occurred in comparison to sound dentin

The Efficacy of Silver Diamine Fluoride as a Caries Preventive Agent on Permanent Teeth: A Scoping Review

“…On the other hand, the application of SDF on 20 specimens of demineralized dentin showed a longitudinal section of dentin with SDF deposition inside the exposed dentin tubules. 54 This is consistent with the findings of Farhadian et al, who explained that SDF solution can be used for dentin treatment by forming a layer of Ag-protein conjugate on the dentin surface, which then precipitates inside the dentin tubules. 50 Demineralized dentin has a larger number of open collagen fibers, which affects the amount of silver ion absorption because the soluble silver from SDF has strong bonding to collagen, resulting in the removal of the smear layer and causing deeper deposition of silver particles within the dentin tubules.…”

“…50 Demineralized dentin has a larger number of open collagen fibers, which affects the amount of silver ion absorption because the soluble silver from SDF has strong bonding to collagen, resulting in the removal of the smear layer and causing deeper deposition of silver particles within the dentin tubules. 54,55 The study conducted by Srisomboon et al on 52 demineralized dentin specimens detected crystal deposits in the peritubular dentin and dentin tubules containing Ca, P, and F. Additionally, crystals consisting of Ag and Cl were also observed in their research. 45 This aligns with the findings of Yu et al, who detected the peak diffraction of hydroxyapatite in their study.…”

“… 50 Demineralized dentin has a larger number of open collagen fibers, which affects the amount of silver ion absorption because the soluble silver from SDF has strong bonding to collagen, resulting in the removal of the smear layer and causing deeper deposition of silver particles within the dentin tubules. 54 55 …”