The effect of Sinensetin and Imperatorin on A-549 lung cancer cell viability in vitro

Abstract

and imperatorin [Sigma-Aldrich European Pharmacopoeia (EP) Reference Standard] were dissolved in DMSO to prepare a 5000 µM stock solution. Various combinations of sinensetin and imperatorin were prepared as follows: Sin 60 µM 9 and Imp 30 µM 11 and a combination of Sin:Imp 30:30 µM, Sin:Imp 50:50 µM, and Sin: Imp 60:30 µM. MTT assayA 5 mg/ml MTT (Sigma-Aldrich, Missouri, USA) stock solution was prepared by adding 50 mg of MTT to 10 mL sterile PBS (Sigma-Aldrich) in a sterile universal, stored in the dark at -20 o C and preheated to 25-26 °C prior to use. The MTT solution was filter sterilized using a 0.2 µm syringe filter

Anti-Cancer Potential of Sinensetin, a Bioactive Compound against Human Cervical Carcinoma Cells via its Molecular Mechanism of Apoptosis

“…Sinensetin has been shown in several in vivo and in vitro experiments to exhibit great selectivity, strong efficacy against tumor cells, and low toxicity against normal cells. 13,14 While sinensetin is frequently observed in the treatment of a number of tumors, the impact on cervical cancer has not yet been precisely established, and the probable mechanisms are still unclear. We initially looked at sinensetin's ability to inhibit the proliferation of the HeLa cell line in the current investigation.…”