The effect of single and repeated UVB radiation on rabbit lens

Fris, Miroslav; Čejková, Jitka; Midelfart, Anna

doi:10.1007/s00417-007-0747-6

Cited by 7 publications

(6 citation statements)

References 34 publications

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“…As to lens damage, Bergmanson and Soderberg [27] reported that lens proteins changed after extended low doses of UVR exposure. Other studies have reported that a dose of 3.12 kJ/ m 2 UVB radiation had harmful effects on the lenticular tissue of rabbit eyes [28,29]. Moreover, Mody et al [30] reported that lens damage in albino rats would be larger under repeated exposure compared to a single exposure of the same dose of UVB.…”

Section: Discussionmentioning

confidence: 95%

The enhancement of biological ocular UV radiation on beaches compared to the radiation on grass

Liu

Wang

Gao

et al. 2014

Journal of Photochemistry and Photobiology B: Biology

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Section: Discussionmentioning

confidence: 95%

The enhancement of biological ocular UV radiation on beaches compared to the radiation on grass

Liu

Wang

Gao

et al. 2014

Journal of Photochemistry and Photobiology B: Biology

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“…Within the cell, lipid peroxides can damage DNA [75], induce a drop in total glutathione and dramatic change in the redox ratio of glutathione, and lead to the appearance of new fluorophores and large protein aggregates with low solubility (clouding matrix) in the lens matter [21,[76][77][78][79][80]. Some authors advanced the hypothesis that H 2 O 2 , free radical (O ÀÁ 2 , OH·), and singlet ( 1 D g O 2 ) forms of oxygen are toxic and may be generated in excessive amounts in several experimental cataracts and in human senile cataract as a consequence of lens photooxidation [40,81], radical generation by xenobiotics [59,[82][83][84][85], or impaired enzymatic and nonenzymatic defenses that exist in the eye lens [25,[86][87][88][89][90]. The lens is well equipped with enzymatic antioxidant protective systems (i.e., superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPO)) and endogenous free radical scavengers (e.g., glutathione and ascorbic acid), which both lose their efficiency with age and in cataracts [82,86,[91][92][93][94][95][96][97][98].…”

Section: O X I D a T I V E D A M A G E L I P I D P E R O X I D A T mentioning

confidence: 99%

Biomarkers and special features of oxidative stress in the anterior segment of the eye linked to lens cataract and the trabecular meshwork injury in primary open‐angle glaucoma: challenges of dual combination therapy with N‐acetylcarnosine lubricant eye drops and oral formulation of nonhydrolyzed carnosine

Babizhayev

2011

Fundamemntal Clinical Pharma

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The implication of oxidative stress associated with increased oxidant production in mammalian and human cells characterized by the release of free radicals, resulting in cellular degeneration, is involved in many ocular diseases, such as age-related macular degeneration, retinopathy of prematurity, retinal light damage, primary open-angle glaucoma (POAG), and cataract. Cataract is the leading cause of blindness, accounting for 50% of blindness worldwide. Glaucoma, the leading cause of irreversible blindness, is considered as a progressive optic neuropathy often caused by elevated intraocular pressure (IOP) consequent to abnormally high resistance to aqueous humor (AH) drainage via the trabecular meshwork (TM) and Schlemm's canal. Morphological and biochemical analyses of the TM of patients with POAG revealed the loss of cells, increased accumulation of extracellular matrix proteins (ECM), changes in the cytoskeleton, cellular senescence, and the process of subclinical inflammation. The TM is the target tissue of glaucoma in the anterior chamber, and the development and progression of glaucoma are accompanied by the accumulation of oxidative damage in this tissue. The separate studies were conducted to comparatively evaluate the sensitivity to oxidative stress and lipid peroxidation (LPO) of anterior chamber tissues including TM. Accumulation of the primary, secondary, and end products of LPO (diene and triene conjugates, Schiff's bases) was noted in the studied extracts. Significant differences in the levels of all mentioned LPO products in comparison with the control were observed. The data may be considered as an evidence of LPO participation in the destruction of the trabecule and Schlemm's canal in POAG. Treatment of TM cells with oxidative stress induced POAG-typical changes such as ECM accumulation, cell death, disarrangement of the cytoskeleton, advanced senescence, and the release of inflammatory markers. By pretreatment with antioxidants, prostaglandin analogs, beta-blockers, or local carbonic anhydrase inhibitors, these effects were markedly reduced. Oxidative stress can induce characteristic glaucomatous TM changes, and these oxidative stress-induced TM changes can be minimized by the use of antioxidants and IOP-lowering substances. It is tempting to speculate that the prevention of oxidative stress exposure to the TM may help to reduce the progression of POAG. The author's laboratory has developed and patented the dual combination therapy with N-acetylcarnosine lubricant eye drops and oral formulation of nonhydrolyzed carnosine in ripe cataracts and POAG. The specific regimen for the treatment in each stage of age-related ophthalmic disease has been taken up. In the treatment of POAG, this dual therapy can be combined with conventional antiglaucoma therapy with beta-blocking and/or adrenergic agonist medicines providing the significant IOP-lowering effect and significant increase in outflow facility. The developed therapy is a prominent management care of the glaucomatous neurodegeneration.

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“…The rabbit model is still being used to examine the effects of ultraviolet radiation on the development of cataracts (Hightower and McCready, 1992). While variations in method vary, in general the model is created by exposing the corneas of anesthetized rabbits to ultraviolet light from a distance of 0.05 m for various frequencies and durations to create different doses of radiation exposure (Fris et al, 2008). The rabbit model is being used to develop treatments for or protection against radiation-induced lens damage (Bigsby et al, 2009;Sasaki et al, 1998).…”

Section: Rabbit Modelmentioning

confidence: 99%