Incomplete healing of a cesarean scar is a mid-term complication of cesarean section and is widely known to be associated with many gynecological symptoms. In this study, we evaluated the correlation between uterine closure suture techniques in the development of uterine scar defect after cesarean section. Material and Methods: The study was conducted prospectively with 131 patients who applied to single center between April 2022 and September 2022, who had an emergency or elective cesarean section indication and had their first cesarean section. Three types of suture techniques were applied to the cesarean section: 42 with single-layer locking (Group 1), 44 with modified Lembert (Group 2), and 45 with sutures continuing from the lateral to the medial (Group 3). Scar thickness-hyperechogenicity, myometrial thickness-vascularization, operating time and number of sutures used for closure of the uterus evaluated by transvaginal ultrasonography (TVUSG) in patients who were called for control at the postoperative 6th week after cesarean section were compared between the groups. Results: The scar thickness detected by TVUSG at postoperative 6th week; in the modified Lembert suture technique, it was found to be less than the other two suture types (p=0.001). Myometrial thickness was also observed to be less in the modified Lembert suture technique compared to the other two groups (p=0.019). Conclusion: In this study, it was shown that the uterine scar and myometrium thickness were thinner and the scar hypercogeneity was less in the modified Lembert closure suture technique in the evaluation performed with TVUSG at the 6 th week.