P. 2005. Acoustic detection of a scallop bed from a single-beam echosounder in the St. Lawrence. e ICES Journal of Marine Science, 62: 966e983.Single-beam seabed echoes combined with epi-macrobenthos photographs were used to remotely detect a scallop bed and characterize the specific acoustic signal of Iceland scallop (Chlamys islandica). A dense scallop bed was surveyed in 2002, with a QTC VIEW Series IV acoustic ground-discrimination system (AGDS) connected to a 38 kHz, 7( split-beam SIMRAD EK60 scientific echosounder. In 2003, a 50 kHz, 42( single-beam SUZUKI ES-2025 echosounder was connected to a QTC VIEW Series V AGDS. The QTC VIEW data were analysed with QTC IMPACT following the standard procedures and classified into acoustic classes. Several approaches were tested: unsupervised and supervised survey strategies directed to specific benthic communities. The SIMRAD EK60 seabed volumebackscattering strength (S v ) was submitted to a principal component analysis (PCA), before and after removal of a depth trend, and the scores on the first 10 principal components were classed by a K-means cluster analysis. The same seabed S v data were submitted to stepwise discriminant analysis whose training data sets were defined with the ground-truth photographs using different groupings: biotope types, community types, and finally scallop-density classes. All the QTC AGDS approaches failed to reveal the scallop bed, community structures, or biotopes. The QTC classifications mimicked the bathymetry with a strong correlation of the acoustic classes with depth. The seabed S v PCA C K-means approach presented similar depth-dependence, but, the PCA C K-means on the S v residuals revealed the scallop bed. The discriminant analysis was the best solution for the scallop density with a general classification success rate of 75% and up to 91% for the highest density class. The S v signature of the scallop bed is presented, and the most discriminant part of the acoustic signal is identified.Published by Elsevier Ltd on behalf of International Council for the Exploration of the Sea.