The effect of soya protein supplement enriched with
three amino acids on duodenal flow of soluble nitrogen
fractions and amino acids in dairy cows

Křížová, Ludmila; Richter, Michal; Třináctý, J.; Šterc, J.; Hadrová, Sylvie

doi:10.22358/jafs/66756/2007

Cited by 3 publications

(4 citation statements)

References 19 publications

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“…Nevertheless, results of our previous work confirmed the functionality of produced copolymer-coated rumen protected tablets that were used in the present experiment by significantly increasing duodenal flow of supplemented AA (Křížová et al 2007) as well as by increase in milk yield and milk protein yield (Třináctý et al 2006). …”

Section: Discussionsupporting

confidence: 85%

Effect of Ruminally-protected leucine supplement on milk yield and plasma amino acids in dairy cows

Křížová¹,

Třináctý²,

Richter³

2008

AFSci

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The aim of this study was to determine the influence of leucine supplement in the form of rumen-protected tablets on milk yield and composition and plasma amino acids in four high-yielding lactating Holstein cows. The experiment was carried out as a cross-over procedure and was divided into 4 periods of 14 d (10 d preliminary period and 4 d experimental period). Cows were fed ad libitum a diet based on maize silage, lucerne hay and a supplemental mixture. The diet, defficient in methionine, lysine, and leucine, was supplemented with methionine+lysine (Control) or methionine+lysine+leucine (Leu) in rumen protected form. The dry matter intake, milk yield and milk yield expressed in energy corrected milk did not differ significantly between the treatments. Milk protein content and yield did not show statistically significant variation. The contents and yield of casein, fat, lactose and urea were unaffected by the treatment. Blood metabolites did not vary between the treatments. The introduction of Leu resulted in higher plasma levels of proline (p<0.05) in comparison to Control. The calculated leucine defficiency in maize silage-concentrate diet used in the present experiment was approx 5.5%. The results of this experiment suggest that under the given conditions, leucine was not a limiting amino acid.

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Section: Discussionsupporting

confidence: 85%

Effect of Ruminally-protected leucine supplement on milk yield and plasma amino acids in dairy cows

Křížová¹,

Třináctý²,

Richter³

2008

AFSci

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“…In the previous study focused on the estimation of duodenal fl ow of soluble nitrogen fractions in the same experiment (Křížová et al, 2007), it has been found that in the case of ruminally protected tablets sampling from the duodenal cannula was unrepresentative. Thus for the evaluation of actual and predicted amino acid content in the duodenal digesta only the data from the control group of animals (C) receiving the supplement in the powder form were used.…”

Section: Experiment Animals Feedmentioning

confidence: 99%

“…For the evaluation of actual and predicted amino acid content in the duodenal digesta, the data from previous studies (Třináctý et al, 2006, Křížová et al, 2007 were used. Cows, treatments and feeding regiment have been described previously (Třináctý et al, 2006).…”

Section: Experiment Animals Feedmentioning

confidence: 99%

“…Processing of feed, feed refusals and duodenal chymus samples for analyses has been described in details elsewhere (Křížová et al, 2007). In feed and feed refusals samples the following parameters were estimated according to AOAC (1984): dry matter (DM), ash, crude protein (CP), fat (F) and crude fibre (CF).…”

Section: Collection Processing and Analyses Of Samplesmentioning

confidence: 99%

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Comparison of actual and predicted amino acid contents in the duodenal digesta of dairy cows

Richter¹,

Třináctý²,

Křížová³

2014

Acta Univ. Agric. Silvic. Mendelianae Brun.

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RICHTER, M., TŘINÁCTÝ, J., KŘÍŽOVÁ, L.: Comparison of actual and predicted amino acid contents in the duodenal digesta of dairy cows. Acta univ. agric. et silvic. Mendel. Brun., 2010, LVIII, No. 5, pp. 313-320 In this experiment on three dairy cows with the ruminal and duodenal T-cannulas, the actual and predicted amino acid (AA) profi les of the protein fraction fl owed to the small intestine were compared. The prediction was calculated by two methods: with the use of mean published AA profi le of microbial protein and of experimentally determined one. The actual AA profi le of digesta protein was corrected for glycine (Gly) of bile origin. In comparison to the published AA profi le of microbial protein the use of the actual one did not contribute to an improvement of prediction accuracy (mean prediction error: 7.36 vs. 7.54 %, respectively). Inaccurate determination of AA composition of undegraded feed protein and the insuffi cient correction for Gly of bile origin were the probable sources of the residual variability. amino acids, dairy cow, duodenum, microbial protein, prediction

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Effect of rumen-protected methionine, lysine or both on milk production and plasma amino acids of high-yielding dairy cows

Třináctý¹,

Křížová²,

Richter³

et al. 2009

Czech J. Anim. Sci.

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ABSTRACT:The objective of this study was to determine the effect of supplemental lysine (Lys), methionine (Met) or both amino acids added in the form of rumen-protected (RP) tablets with copolymer coating to a diet of dairy cows on yield and composition of milk and concentration of plasma amino acids (AA). The experiment was carried out on four high-yielding lactating Holstein cows with average milk production of 33.5 kg/day in the form of Latin square design. The four treatments were as follows: C -control without AA supplementation, L -control plus supplement of RP Lys, M -control plus supplement of RP Met and ML -control plus supplement of RP Met and Lys. The experiment was divided into 4 periods. Each period (14 days) consisted of 10-day preliminary period and 4-day experimental period. Cows were fed a diet based on maize silage, lucerne hay and supplemental mixture. Average milk yield recorded in ML was 34.18 kg and was higher than that recorded in L or M (32.46 kg and 32.13 kg, respectively P < 0.05) and tended to be higher than in C (33.33 kg, P > 0.05). The content of protein and casein was higher in L and ML in comparison with C (P < 0.05) and tended to be higher than in M (P > 0.05). Protein yield in ML (1 054 g/day) was higher than that found in C, L or M (990, 998 or 968 g/day, respectively, P < 0.05). The same response was found for casein yield (P < 0.05). Although the proportion of individual casein fractions was not affected by the treatment, the yield of α-and β-casein differed (P < 0.05) while the yield of κ-casein was not affected by the treatment. Concentrations of blood metabolites, except for betahydroxybutyrate (BHB), were not changed. Plasma Met concentrations were increased (P < 0.05) in the M and ML group. Similar, but insignificant increases (P > 0.05) were also observed in plasma Lys in the L and ML group.

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The effect of soya protein supplement enriched with three amino acids on duodenal flow of soluble nitrogen fractions and amino acids in dairy cows

Cited by 3 publications

References 19 publications

Effect of Ruminally-protected leucine supplement on milk yield and plasma amino acids in dairy cows

Effect of Ruminally-protected leucine supplement on milk yield and plasma amino acids in dairy cows

Comparison of actual and predicted amino acid contents in the duodenal digesta of dairy cows

Effect of rumen-protected methionine, lysine or both on milk production and plasma amino acids of high-yielding dairy cows

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