The effect of starvation on the ultrastructure of the digestive cells of Dolops ranarum (Stuhlmann, 1891) (Crustacea: Branchiura)

Tam, Quinton; Avenant‐Oldewage, Annemariè

doi:10.1016/j.asd.2009.03.002

Cited by 10 publications

(4 citation statements)

References 37 publications

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“…Their divisions have been observed in crustaceans in the distal and medial zones, while no mitosis was observed in the proximal zone of the hepatopancreatic tubules [ 22 ]. However, in some cases mitotic divisions of E-cells were not confirmed [ 3 ] or these cells have not been observed in the midgut epithelium [ 4 , 15 , 16 , 19 ]. It has been suggested that a short life span causes epithelial degeneration to be rare and therefore the midgut epithelium can survive without regeneration.…”

Section: Discussionmentioning

confidence: 99%

“…However, there are still many controversies connected with the functions of cells that have been described; some authors seemed to have difficulties in differentiating all of the types of cells. Therefore, in the literature we can still find data that asserts that all of the cells that form the midgut epithelium in Crustacea may represent the stages of cell differentiation [ 7 , 10 , 13 – 16 ].…”

Section: Introductionmentioning

confidence: 99%

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Structure and Ultrastructure of the Endodermal Region of the Alimentary Tract in the Freshwater Shrimp Neocaridina heteropoda (Crustacea, Malacostraca)

et al. 2015

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The freshwater shrimp Neocaridina heteropoda (Crustacea, Malacostraca, Decapoda) originates from Asia and is one of the species that is widely available all over the world because it is the most popular shrimp that is bred in aquaria. The structure and the ultrastructure of the midgut have been described using X-ray microtomography, transmission electron microscopy, light and fluorescence microscopes. The endodermal region of the alimentary system in N. heteropoda consists of an intestine and a hepatopancreas. No differences were observed in the structure and ultrastructure of males and females of the shrimp that were examined. The intestine is a tube-shaped organ and the hepatopancreas is composed of two large diverticles that are divided into the blind-end tubules. Hepatopancreatic tubules have three distinct zones – proximal, medial and distal. Among the epithelial cells of the intestine, two types of cells were distinguished – D and E-cells, while three types of cells were observed in the epithelium of the hepatopancreas – F, B and E-cells. Our studies showed that the regionalization in the activity of cells occurs along the length of the hepatopancreatic tubules. The role and ultrastructure of all types of epithelial cells are discussed, with the special emphasis on the function of the E-cells, which are the midgut regenerative cells. Additionally, we present the first report on the existence of an intercellular junction that is connected with the E-cells of Crustacea.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Structure and Ultrastructure of the Endodermal Region of the Alimentary Tract in the Freshwater Shrimp Neocaridina heteropoda (Crustacea, Malacostraca)

et al. 2015

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“…Morphological alterations of the HP, such as reduction in the tubular area and enlargement of the lumen area, variation in the number of B‐cells, F‐cells and R‐cells, infiltration of haemocytes in the intertubular spaces, have been described by different authors in case of pathogens, food deprivation, changes in salinity and food ingredients (Nakamura ; Sánchez‐Paz et al . ; Tam & Avenant‐Oldewage ; Diaz, Sousa & Petriella ; Masson, Diaz & Petriella ; Sacristán et al . ).…”

Section: Methodsmentioning

confidence: 99%

Use of computer‐assisted image analysis for semi‐quantitative histology of the hepatopancreas in whiteleg shrimp Penaeus vannamei (Boone)

Cervellione

McGurk

Eriksen

et al. 2016

Journal of Fish Diseases

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Despite the increasing use of novel molecular techniques in pathology, histology remains the standard method for monitoring tissue alterations and for assessing pathology. Histopathological evaluation is generally laborious and subjective with risk of discrepancies in semi-quantitative scoring between pathologists. In contrast, computer-assisted image analysis (CAIA) is potentially faster, more objective and thus suitable for routine screening. Limited research has been carried out on CAIA in crustacean histopathology, and the methods described were not fully automated. Therefore, the aim of this study was to develop CAIA in whiteleg shrimp (Penaeus vannamei) for the study of the hepatopancreas. Paraffin sections were immunohistochemically stained with monoclonal antibodies WSH8 against haemocytes and counterstained with Mayer's haematoxylin for detection of haemocytes and B-cell vacuoles, and modified toluidine blue protocol was used for detection of F-cells; frozen sections were stained with Oil Red O for detection of lipid droplets within R-cells. Visiopharm software was used to develop and validated protocols for the quantification of morphological parameters (areas of haemocyte infiltration, F-cells, B-cell vacuoles, lipid droplets and their ratios to total tissue area and total lumen area). These protocols enable the future use of CAIA for determination of the nutritional and pathological condition of this organ.

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“…From then she no longer published under her maiden name, but as Avenant-Oldewage, beginning with descriptions of the morphologies of the reproductive (Avenant-Oldewage and van As 1990a) and digestive (Avenant-Oldewage and van As 1990b) systems of D. ranarum. Other papers on D. ranarum were published focusing on the damage caused to the skin of the catfish C. gariepinus (Avenant-Oldewage 1994) and the effect of starvation on the ultrastructure of digestive cells (Tam and Avenant-Oldewage 2009). Dolops ranarum is most likely the best-studied African branchiuran.…”

Section: Aquatic Parasitology At Rand Afrikaans University/ Universitmentioning

confidence: 98%

A brief history of freshwater fish parasitology in southern Africa

2015

African Zoology

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The effect of starvation on the ultrastructure of the digestive cells of Dolops ranarum (Stuhlmann, 1891) (Crustacea: Branchiura)

Cited by 10 publications

References 37 publications

Structure and Ultrastructure of the Endodermal Region of the Alimentary Tract in the Freshwater Shrimp Neocaridina heteropoda (Crustacea, Malacostraca)

Structure and Ultrastructure of the Endodermal Region of the Alimentary Tract in the Freshwater Shrimp Neocaridina heteropoda (Crustacea, Malacostraca)

Use of computer‐assisted image analysis for semi‐quantitative histology of the hepatopancreas in whiteleg shrimp Penaeus vannamei (Boone)

A brief history of freshwater fish parasitology in southern Africa

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