The effect of starvation stress on <i>Lactobacillus brevis</i> L62 protein profile determined by <i>de novo</i> sequencing in positive and negative mass spectrometry ion mode

Butorac, Ana; Dodig, Ivana; Bačun-Družina, Višnja; Tishbee, A.; Mrvčić, Jasna; Hock, Karlo; Diminić, Janko; Cindrić, Mario

doi:10.1002/rcm.6528

Cited by 13 publications

(11 citation statements)

References 24 publications

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“…In addition, some of these differentially regulated Lactococcus lactis genes were functionally related to glucose exhaustion, including the induction of the arginine deiminase pathway as well as alternative sugar transport and metabolic pathways . Recently, Butorac et al used de novo sequencing in positive and negative mass spectrometry ion modes to investigate the adaptation of Lactobacillus brevis to nutrient deprivation and found that numerous proteins engaged in glucose and amino acid catabolism were differentially expressed after long-term starvation; however, genomic analysis was not performed in this study …”

Section: Introductionmentioning

confidence: 85%

“…9 Recently, Butorac et al used de novo sequencing in positive and negative mass spectrometry ion modes to investigate the adaptation of Lactobacillus brevis to nutrient deprivation and found that numerous proteins engaged in glucose and amino acid catabolism were differentially expressed after long-term starvation; however, genomic analysis was not performed in this study. 10 L. casei Zhang, a strain isolated from koumiss samples collected from Inner Mongolia, China, has been considered a probiotic bacterium via selection tests. 11−13 A proteomic study to identify proteins expressed by L. casei Zhang in the exponential and stationary phases revealed that the differentially expressed proteins were mainly categorized as the key components of central and intermediary metabolism.…”

Section: ■ Introductionmentioning

confidence: 99%

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Integrative Genomic and Proteomic Analysis of the Response of Lactobacillus casei Zhang to Glucose Restriction

Hui

Cao

et al. 2018

J. Proteome Res.

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Nutrient starvation is an important survival challenge for bacteria during industrial production of functional foods. As next-generation sequencing technology has greatly advanced, we performed proteomic and genomic analysis to investigate the response of Lactobacillus casei Zhang to a glucose-restricted environment. L. casei Zhang strains were permitted to evolve in glucose-restricted or normal medium from a common ancestor over a 3 year period, and they were sampled at 1000, 2000, 3000, 4000, 5000, 6000, 7000, and 8000 generations and subjected to proteomic and genomic analyses. Genomic resequencing data revealed different point mutations and other mutational events in each selected generation of L. casei Zhang under glucose restriction stress. The differentially expressed proteins induced by glucose restriction were mostly related to fructose and mannose metabolism, carbohydrate metabolic processes, lyase activity, and amino-acid-transporting ATPase activity. Integrative proteomic and genomic analysis revealed that the mutations protected L. casei Zhang against glucose starvation by regulating other cellular carbohydrate, fatty acid, and amino acid catabolism; phosphoenolpyruvate system pathway activation; glycogen synthesis; ATP consumption; pyruvate metabolism; and general stress-response protein expression. The results help reveal the mechanisms of adapting to glucose starvation and provide new strategies for enhancing the industrial utility of L. casei Zhang.

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Section: Introductionmentioning

confidence: 85%

Section: ■ Introductionmentioning

confidence: 99%

Integrative Genomic and Proteomic Analysis of the Response of Lactobacillus casei Zhang to Glucose Restriction

Hui

Cao

et al. 2018

J. Proteome Res.

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“…Two-dimensional gel electrophoresis (2-DE) was performed as described by Butorac et al . ( 10 ). Isoelectric focusing (IEF) was performed using Flatbed IEF System (Cleaver Scientific, Rugby, UK).…”

Section: Methodsmentioning

confidence: 99%

Comparison of Traditional and Industrial Sausages Baranjski Kulen and Kulenova Seka Using Comprehensive Proteome, Peptidome and Metabolome Techniques

Šimunec

Bertoša²,

Šporec³

et al. 2022

Food Technol. Biotechnol. (Online)

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Research background. Baranjski kulen is one of the most popular fermented meat sausages originating from Croatia. It has protected geographical indication, and is traditionally produced in the Baranja region of Croatia. Kulenova seka is a fermented sausage very similar to Baranjski kulen, but it has a different caliber and consequently, a shorter time of production. In recent decades, due to the high demand and popularity of these products, industrially produced Baranjski kulen and Kulenova seka have become available on the market. This work aims to identify specific characteristics of traditional and industrial sausages, Baranjski kulen and Kulenova seka on proteome, peptidome and metabolome level which could potentially lead to better optimization of the industrial production process in order to obtain an equivalent to the traditional product. Experimental approach. Protein profiles of Baranjski kulen and Kulenova seka (traditional and industrial) were analysed using two-dimensional gel electrophoresis followed by differential display analysis and protein identification using mass spectrometry. Peptidomics profiling analysis was performed via liquid chromatography-tandem mass spectrometry. Furthermore, aroma profiles were investigated via headspace solid phase microextraction and gas chromatography-mass spectrometry. Results and conclusions. The major identified characteristics of each product were: industrial Baranjski kulen - specific degradation of MYH1 and TITIN, overabundance of stress-related proteins and increased phenylalanine degradation; traditional Baranjski kulen - decreased concentration of phenylalanine and overabundance of ALDOA and CAH3; industrial Kulenova seka - specific MYH4 and HBA degradation process; traditional Kulenova seka - overabundance of DPYD and MYL1, degradation of ALBU and MYG, decreased concentrations of almost all free amino acids and increased amounts of smoke derived volatile compounds. Novelty and scientific contribution. In this preliminary communication, the first insights into protein degradation processes and generation of peptides, free amino acids and aroma compounds of industrial and traditional Baranjski kulen and Kulenova seka are presented. Although further research is needed to draw general conclusions, the specific profile of proteins, peptides, amino acids, and volatile compounds represents the first step in the industrial production of sausages that meet the characteristics of traditional flavour.

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“…Glycolic and pentose phosphate pathway enzymes were induced, indicating that the metabolism was switched to scavenging other carbon sources in the medium, which included Tween 80 and amino acids. A recent study used a novel negative chemically activated fragmentation/positive chemically activated fragmentation (CAF−/CAF+) technique for the identification of differentially expressed proteins in the proteome of L. brevis after 75 days of starvation (Butorac et al ., ). This revealed that starved L. brevis metabolized arginine, glycine and histidine from dead cells as alternative nutrient sources.…”

Section: Proteomics To Study the Stressful Life Of Lactobacillimentioning

confidence: 97%

Proteomics and the stressful life of lactobacilli

Hussain

Nezhad²,

Sheng

et al. 2013

FEMS Microbiol Lett

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Lactobacilli occupy specific ecological niches, where they represent a major component of foods, human and animal microbial communities. Employing these bacteria in industrial fermentations or for human health benefits exposes them to certain life-threatening conditions where their ability to adapt plays a key role in their survival and continued microbial activity. Since the postgenomic era began, proteomics has become the first choice among research approaches available for environmental adaptation and stress response investigators. The latest developments in the applications of proteomics to understand physiological changes in Lactobacillus species under harsh conditions are remarkable.

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The effect of starvation stress on Lactobacillus brevis L62 protein profile determined by de novo sequencing in positive and negative mass spectrometry ion mode

Cited by 13 publications

References 24 publications

Integrative Genomic and Proteomic Analysis of the Response of Lactobacillus casei Zhang to Glucose Restriction

Integrative Genomic and Proteomic Analysis of the Response of Lactobacillus casei Zhang to Glucose Restriction

Comparison of Traditional and Industrial Sausages Baranjski Kulen and Kulenova Seka Using Comprehensive Proteome, Peptidome and Metabolome Techniques

Proteomics and the stressful life of lactobacilli

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