The effect of stem cell from human exfoliated deciduous teeth on T lymphocyte proliferation

Abstract

Background:Mesenchymal stem cells (MSC), a specific type of adult tissue stem cell; have the immunosuppressive effects that make them valuable targets for regenerative medicine and treatment of many human illnesses. Hence, MSC have been the subject of numerous studies. The classical source of MSC is adult bone marrow (BM). Due to many shortcomings of harvesting MSC from BM, finding the alternative sources for MSC is an urgent. Stem cells from human exfoliated deciduous teeth (SHED) are relative new MSC populations that fulfill these criteria but their potential immunosuppressive effect has not been studied enough yet. Thus, in this work the effect of SHED on the proliferation of in vitro activated T lymphocytes were explored.Materials and Methods:In this study, both mitogen and alloantigen activated T cells were cultured in the presence of different numbers of SHED. In some co-cultures, activated T cells were in direct contact to MSCs and in other co-cultures; they were separated from SHED by a permeable membrane. In all co-cultures, the proliferation of T cells was measured by ELISA Bromodeoxyuridine proliferation assay.Results:In general, our results showed that SHED significantly suppress the proliferation of activated T cells in a dose-dependent manner. Moreover, the suppression was slightly stronger when MSCs were in physical contact to activated T cells.Conclusion:This study showed that SHED likewise other MSC populations can suppress the activation of T lymphocytes, which can be used instead of BM derived MSCs in many investigational and clinical applications.

Immunoenhancing Constituents of Poria cocos

“…The phagocytic index of CPF was significantly higher than the control group, revealed that saccharides may be the main active compositions for the innate immunity. The transformation level of lymphocyte could reflect the ability of immune system and was one of the major indexes in evaluating the cytoimmunity (Alipour et al, 2014). The results showed that the PBL transformation rates of EAF group and CPF group from the third day to the sixth day of the final intramuscular injection with PHA, exceeded that of control group, revealing that saccharides and triterpenoids of Poria are the active components.…”

Tissue Engineering in Stomatology: A Review of Potential Approaches for Oral Disease Treatments

“…In addition to bone mesenchymal stem cells (BMSCs) ( Nakamura et al, 2013 ; Yoo et al, 2013 ; Selvasandran et al, 2018 ; Li Y. et al, 2019 ; Xu M. et al, 2019 ) and adipose-derived stromal cells (ADSCs) ( Yoon et al, 2011 ; Yao et al, 2012 ; Mihaila et al, 2014 ; Zhu et al, 2019 ; Xu et al, 2020 ), various MSCs have also been derived from teeth in recent years ( Volponi et al, 2010 ), such as dental pulp stem cells (DPSCs) ( Chen Y.-Y. et al, 2016 ; Lambrichts et al, 2017 ), stem cells from human exfoliated deciduous teeth (SHEDs) ( Alkaisi et al, 2013 ; Alipour et al, 2014 ; Behnia et al, 2014 ; Sugimura-Wakayama et al, 2015 ), periodontal ligament stem cells (PDLSCs) ( Kim et al, 2010 ; Chen F.-M. et al, 2016 ; Panduwawala et al, 2017 ), stem cells from apical papilla (SCAPs) ( Bakopoulou et al, 2011 ; Somoza et al, 2017 ; Yang et al, 2018 ; Yang et al, 2020 ; Shen et al, 2021 ), dental follicle cells (DFCs) ( Tian et al, 2015 ; Yildirim et al, 2016 ; Lima et al, 2017 ), and gingival mesenchymal stem cells (GMSCs) ( Zhang et al, 2009 ; Ansari et al, 2016 ; Shi et al, 2017 ; Rao et al, 2019 ; Liu X. et al, 2020 ) ( Table 1 ). Scaffolds provide a suitable space for cell growth and functions.…”