2011
DOI: 10.1016/j.idairyj.2010.12.001
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The effect of storage at 25 °C on proteins in human milk

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Cited by 12 publications
(9 citation statements)
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“…Protein concentrations of human milk samples were determined using a Bicinchoninic acid kit (Sigma-Aldrich, NSW, Australia), as described in a previous study …”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…Protein concentrations of human milk samples were determined using a Bicinchoninic acid kit (Sigma-Aldrich, NSW, Australia), as described in a previous study …”
Section: Methodsmentioning
confidence: 99%
“…Gels were fixed and stained as described above. Coomassie stained bands and spots of interest were cut from the gel, destained, and digested as described by Shevchenko et al Mass spectrometry was conducted as described in a previous study using an UltraFlex MALDI-TOF/TOF instrument (Bruker Daltonics, Bremen, Germany) . MS/MS data was imported into the database search engine Mascot (Version 2.3.01, ) and searched against the Swiss-Prot Mammalia database (49 887 sequences).…”
Section: Methodsmentioning
confidence: 99%
“…For the analysis of skimmed milk proteins, 20 μg of protein was loaded. Gels were fixed, stained using Coomassie Blue Silver and scanned as described previously ( 16 ) .…”
Section: Experimental Methodsmentioning
confidence: 99%
“…Bands and spots of interest were cut from the gel, destained and digested in gel with trypsin (Roche Diagnostics), as described by Shevchenko et al ( 18 ) . MS analysis was conducted as described in a previous study ( 16 ) .…”
Section: Experimental Methodsmentioning
confidence: 99%
See 1 more Smart Citation