The effect of streptococcal L-form cultures on lymphocytes

Cook, Josephine; Fincham, W J

doi:10.1016/0024-3205(69)90059-9

Cited by 12 publications

(6 citation statements)

References 3 publications

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“…Streptolysin S itself is not mitogenic. The mitogen present in streptolysin S preparations is a contaminant which can be separated from streptolysin S by chromatography and appears to be clearly distinct from streptolysin S. 5 13,14 More re-cently Francis and Oppenheim3 recorded hyporesponsiveness in peripheral-leukocyte cultures stimulated by pathogenic strains of group A streptococci when patients with RF were compared to normal controls. Our findings of frequent lack of cross-stimulation in mixed-leukocyte cultures using lymphocytes from patients with active rheumatic fever are in accord with these previous documentations of lymphocyte hyporeactivity somehow associated with the acute rheumatic state.…”

Section: Discussionmentioning

confidence: 99%

Decreased Reactivity of Lymphocytes in Mixed-Leukocyte Culture from Patients with Rheumatic Fever

Lueker

Williams

1972

Circulation

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The purpose of this study was to evaluate cellular immune responsivenes.s in patients with acute rheumatic fever (ARF). Peripheral blood lymphocytes were obtained for culture from patients who had ARF within the past 2-3 months. No patient was receiving steroids at the time of the study. Peripheral lymphocytes were also obtained from normal control individuals.Lymphocyte cultures were maintained for 7 days in minimum essential medium with 10% fetal calf serum in a 5% CO2 environment. Cellular responsiveness was checked concomitantly with pokeweed mitogen and/or phytohemagglutinin. Mixed-lymphocyte cultures were studied between patients with ARF and between patients with ARF and normal controls. Normal responses were arbitrarily defined as a threefold increase over baseline counts.ARF cells were capable of stimulating other ARF cells in only three of 14 instances and were able to stimulate control cells in only three of 11 studies. Conversely, ARF lymphocytes were capable of being stimulated by control normal cells in five of 10 experiments. Thus decreased cellular responsiveness and abnormalities in cellular immunity are present in many patients with ARF, since lymphocytes from patients with ARF are usually incapable of stimulating normal or other ARF cells. However, ARF cells are capable of being stimulated by normal control cells in 50% of studies performed. CLOSE HISTOLOGIC inspection of the microscopic cardiac lesions in acute rheumatic fever, such as areas of acute myocarditis or Aschoff bodies themselves, often shows prominent cellular infiltrations with small and medium-sized lymphocytes. It is surprising, therefore, that more attention has not been directed to a study of circulating lymphocytes in individuals afflicted with acute or subacute rheumatic fever. There is indeed a voluminous literature dealing with various

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Section: Discussionmentioning

confidence: 99%

Decreased Reactivity of Lymphocytes in Mixed-Leukocyte Culture from Patients with Rheumatic Fever

Lueker

Williams

1972

Circulation

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“…A noteworthy characteristic of both strains of L-form is their capacity, even after they have been heated to 80°C for 30 min., to cause transformation of lymphocytes (Cook and Fincham, 1969). This action may be caused by the mitogen which is known to be present in SLS and which Taranta, Cuppari and Quagliata (1969) showed to be unrelated to the haemolytic factor.…”

Section: Table VI Precipitating Antibodies Against C 203 U L-forms Inmentioning

confidence: 97%

“…It might be argued that the chronic synovitis produced by SLS could still be due to labilisation of lysosomes in synovial cells even though insufficient SLS reached the chondrocytes to produce the disorganisation of cartilage that is seen with filipin. Against this is the report by Cook and Fincham (1966) that chloroquine did not protect joints injected with SLS from undergoing the chronic inflammatory changes already listed.…”

Section: Table VI Precipitating Antibodies Against C 203 U L-forms Inmentioning

confidence: 98%

“…Post-mortem material was treated as described in a previous paper (Cook and Fincham, 1966). Swabs taken from the jointspost mortem were cultured in digest broth.…”

Section: Necropsiesmentioning

confidence: 99%

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PLATES CXXV-CXXVIII CHRONIC arthritis in rabbits produced by intra-articular injections of streptolysin S (SLS) has already been described by Weissmann et al (1965) and by Cook and Fincham (1966). This paper reports that a similar arthritis has been produced by the L-form of the strain C 203 S which is the strain of group-A streptococcus used initially for the production of SLS, and by the L-form of C 203 U, a non-haemolytic variant that we obtained from Professor A. Bernheimer.Some animals were injected intra-articularly with the parent strain C 203 S so that the changes could be compared with those produced by the L-form.

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confidence: 99%

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