The effect of Streptococcus mutans and Candida glabrata on Candida albicans biofilms formed on different surfaces

Pereira‐Cenci, Tatiana; Deng, Dongsheng; Kraneveld, Eefje A.; Manders, Erik M. M.; Cury, Altair Antoninha Del Bel; Cate, J.M. ten

doi:10.1016/j.archoralbio.2008.02.015

Cited by 149 publications

(145 citation statements)

References 42 publications

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“…Co-culture with P. aeruginosa results in reduced biofilm formation of C. albicans (Holcombe et al, 2010) and this could be owing to excreted phenanzines (Morales et al, 2013) or to bacterial lipopolysaccharides (Bandara et al, 2013). It has been reported that S. mutans can improve growth of C. albicans biofilms (Pereira-Cenci et al, 2008) and that C. albicans profits from lactic acid excreted by S. mutans (Metwalli et al, 2013). The gas chromatography-mass spectrometry data show that monosaccharides released by the extracellular glycosyl-und fructosyltransferases of S. mutans from sucrose (Ajdic et al, 2002) were depleted from the spent medium in dual-species biofilms at 10 h, and thus may have been taken up by C. albicans.…”

Section: Discussionmentioning

confidence: 99%

Cross-feeding and interkingdom communication in dual-species biofilms of Streptococcus mutans and Candida albicans

et al. 2014

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Polymicrobial biofilms are of large medical importance, but relatively little is known about the role of interspecies interactions for their physiology and virulence. Here, we studied two human pathogens co-occuring in the oral cavity, the opportunistic fungus Candida albicans and the caries-promoting bacterium Streptococcus mutans. Dual-species biofilms reached higher biomass and cell numbers than mono-species biofilms, and the production of extracellular polymeric substances (EPSs) by S. mutans was strongly suppressed, which was confirmed by scanning electron microscopy, gas chromatography-mass spectrometry and transcriptome analysis. To detect interkingdom communication, C. albicans was co-cultivated with a strain of S. mutans carrying a transcriptional fusion between a green fluorescent protein-encoding gene and the promoter for sigX, the alternative sigma factor of S. mutans, which is induced by quorum sensing signals. Strong induction of sigX was observed in dual-species biofilms, but not in single-species biofilms. Conditioned media from mixed biofilms but not from C. albicans or S. mutans cultivated alone activated sigX in the reporter strain. Deletion of comS encoding the synthesis of the sigX-inducing peptide precursor abolished this activity, whereas deletion of comC encoding the competence-stimulating peptide precursor had no effect. Transcriptome analysis of S. mutans confirmed induction of comS, sigX, bacteriocins and the downstream late competence genes, including fratricins, in dual-species biofilms. We show here for the first time the stimulation of the complete quorum sensing system of S. mutans by a species from another kingdom, namely the fungus C. albicans, resulting in fundamentally changed virulence properties of the caries pathogen.

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Section: Discussionmentioning

confidence: 99%

Cross-feeding and interkingdom communication in dual-species biofilms of Streptococcus mutans and Candida albicans

et al. 2014

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“…26 The virulence of C. albicans may be influenced not only by its own properties but also by its interactions with cohabitating oral microorganisms. 20 Further research is required to understand the interactions that occur when different species of Candida coexist in a biofilm.…”

Section: Discussionmentioning

confidence: 99%

“…Kirkpatrick et al 4 and Thein et al 19 found that C. albicans established a competitive interaction with C. dubliniensis and C. krusei, respectively, in mixed biofilms formed in vitro. However, Pereira-Cenci et al 20 did not find competitive interactions in dual-species biofilms of C. albicans and C. glabrata grown on different dental materials. Recently, our laboratory developed an in vitro and in vivo study 21 to evaluate the interactions of C. albicans (ATCC 18804) with C. krusei (ATCC 6258) and C. glabrata (ATCC 9030).…”

Section: Introductionmentioning

confidence: 99%

Mixed biofilms formed by C. albicans and non-albicans species: a study of microbial interactions

et al. 2016

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Most Candida infections are related to microbial biofilms often formed by the association of different species. The objective of this study was to evaluate the interactions between Candida albicans and non-albicans species in biofilms formed in vitro. The nonalbicans species studied were: Candida tropicalis, Candida glabrata and Candida krusei. Single and mixed biofilms (formed by clinical isolates of C. albicans and non-albicans species) were developed from standardized suspensions of each strain (10 7 cells/mL), on flat-bottom 96-well microtiter plates for 48 hour. These biofilms were analyzed by counting colony-forming units (CFU/mL) in Candida HiChrome agar and by determining cell viability, using the XTT 2,3-bis (2-methoxy-4-nitro-5-sulphophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide colorimetric assay. The results for both the CFU/mL count and the XTT colorimetric assay showed that all the species studied were capable of forming high levels of in vitro biofilm. The number of CFU/mL and the metabolic activity of C. albicans were reduced in mixed biofilms with non-albicans species, as compared with a single C. albicans biofilm. Among the species tested, C. krusei exerted the highest inhibitory action against C. albicans. In conclusion, C. albicans established antagonistic interactions with non-albicans Candida species in mixed biofilms.

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“…The systemic factors include nutritional deficiencies, malignant tumours, use of broad-spectrum antibiotics, smoking, age, diabetes, disorders of the salivary glands and deficiencies in the immune response. 13,15 In immunocompromised individuals, Candida spp. can cause fatal fungaemias.…”

Section: Declaration Of Interestsmentioning

confidence: 99%

“…can be easily isolated from the oral cavity in approximately 60% of healthy adults. 13,14 Candida albicans primarily colonises the tongue, flowing into the oral mucosa, tooth surfaces, the biofilm and saliva. Its pathogenic action is favoured by local or systemic changes.…”

Section: Declaration Of Interestsmentioning

confidence: 99%

Influence of artificial saliva in biofilm formation of Candida albicans in vitro

et al. 2012

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Due to the increase in life expectancy, new treatments have emerged which, although palliative, provide individuals with a better quality of life. Artificial saliva is a solution that contains substances that moisten a dry mouth, thus mimicking the role of saliva in lubricating the oral cavity and controlling the existing normal oral microbiota. This study aimed to assess the influence of commercially available artificial saliva on biofilm formation by Candida albicans. Artificial saliva I consists of carboxymethylcellulose, while artificial saliva II is composed of glucose oxidase, lactoferrin, lysozyme and lactoperoxidase. A control group used sterile distilled water. Microorganisms from the oral cavity were transferred to Sabouraud Dextrose Agar and incubated at 37 °C for 24 hours. Colonies of Candida albicans were suspended in a sterile solution of NaCl 0.9%, and standardisation of the suspension to 10 6 cells/mL was achieved. The acrylic discs, immersed in artificial saliva and sterile distilled water, were placed in a 24-well plate containing 2 mL of Sabouraud Dextrose Broth plus 5% sucrose and 0.1 mL aliquot of the Candida albicans suspension. The plates were incubated at 37 °C for 5 days, the discs were washed in 2 mL of 0.9% NaCl and placed into a tube containing 10 mL of 0.9% NaCl. After decimal dilutions, aliquots of 0.1 mL were seeded on Sabouraud Dextrose Agar and incubated at 37 °C for 48 hours. Counts were reported as CFU/mL (Log10). A statistically significant reduction of 29.89% (1.45 CFU/mL) of Candida albicans was observed in saliva I when compared to saliva II (p = 0.002, considering p ≤ 0.05).

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The effect of Streptococcus mutans and Candida glabrata on Candida albicans biofilms formed on different surfaces

Cited by 149 publications

References 42 publications

Cross-feeding and interkingdom communication in dual-species biofilms of Streptococcus mutans and Candida albicans

Cross-feeding and interkingdom communication in dual-species biofilms of Streptococcus mutans and Candida albicans

Mixed biofilms formed by C. albicans and non-albicans species: a study of microbial interactions

Influence of artificial saliva in biofilm formation of Candida albicans in vitro

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