The Effect of Stromal Cell-Derived Factor 1 in the Migration of Neural Stem Cells

Xue, Leigang; Wang, Jinkun; Wang, Weimin; Yang, Zhiyong; Hu, Zhulin; Hu, Min; Ding, Peng

doi:10.1007/s12013-014-0103-5

Cited by 30 publications

(18 citation statements)

References 32 publications

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“…Sections containing the least spared white matter were considered the epicenter, and distances up to 2,160-m rostral and caudal to the epicenter were analyzed for each animal. Dziembowska et al, 2005;Lieberam et al, 2005;Xue et al, 2014). The data from this study suggests that this cloned SDF-1␣ not only maintains chemotactic functions, but also can exert greater migratory effects on NSCs in vitro, when secreted at levels exceeding those from MSCs alone.…”

Section: Discussionmentioning

confidence: 70%

SDF-1 overexpression by mesenchymal stem cells enhances GAP-43-positive axonal growth following spinal cord injury

Stewart

Matyas

Welchko

et al. 2017

RNN

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Abstract.Purpose: Utilizing genetic overexpression of trophic molecules in cell populations has been a promising strategy to develop cell replacement therapies for spinal cord injury (SCI). Over-expressing the chemokine, stromal derived factor-1 (SDF-1␣), which has chemotactic effects on many cells of the nervous system, offers a promising strategy to promote axonal regrowth following SCI. The purpose of this study was to explore the effects of human SDF-1␣, when overexpressed by mesenchymal stem cells (MSCs), on axonal growth and motor behavior in a contusive rat model of SCI. Methods: Using a transwell migration assay, the paracrine effects of MSCs, which were engineered to secrete human SDF-1␣ (SDF-1-MSCs), were assessed on cultured neural stem cells (NSCs). For in vivo analyses, the SDF-1-MSCs, unaltered MSCs, or Hanks Buffered Saline Solution (vehicle) were injected into the lesion epicenter of rats at 9-days post-SCI. Behavior was analyzed for 7-weeks post-injury, using the Basso, Beattie, and Bresnahan (BBB) scale of locomotor functions. Immunohistochemistry was performed to evaluate major histopathological outcomes, including gliosis, inflammation, white matter sparing, and cavitation. New axonal outgrowth was characterized using immunohistochemistry against the neuron specific growth-associated protein-43 (GAP-43). Results:The results of these experiments demonstrate that the overexpression of SDF-1␣ by MSCs can enhance the migration of NSCs in vitro. Although only modest functional improvements were observed following transplantation of SDF-1-MSCs, a significant reduction in cavitation surrounding the lesion, and an increased density of GAP-43-positive axons inside the SCI lesion/graft site were found. Conclusion:The results from these experiments support the potential role for utilizing SDF-1␣ as a treatment for enhancing growth and regeneration of axons after traumatic SCI. Research HighlightsMSCs were engineered to overexpress SDF-1␣. SDF-1␣ expression by MSCs enhances the migration of NSCs in vitro. SDF-1␣ expression by MSCs enhanced GAP-43 fibers within the lesion center.

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Section: Discussionmentioning

confidence: 70%

SDF-1 overexpression by mesenchymal stem cells enhances GAP-43-positive axonal growth following spinal cord injury

Stewart

Matyas

Welchko

et al. 2017

RNN

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“…Heparanase released from MSCs also activates Integrin beta1/HIF-2alpha/Flk-1 signaling and stem cell migration [55,56]. Many stem cell populations utilize the SDF-1/CXCR4 axis to effect migration [57][58][59][60][61]. The release of progenitor cell populations from their niches by heparanase-1 is consistent with emerging roles for heparanase-1 and 2 in the promotion of wound repair [62,63].…”

Section: Discussionmentioning

confidence: 62%

Perlecan Delineates Stem Cell Niches in Human Foetal Hip, Knee and Elbow Cartilage Rudiments and Has Potential Roles in the Regulation of Stem Cell Differentiation

Melrose¹

2016

SRDT

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Citation: Smith SM, Melrose J (2016) Perlecan Delineates Stem Cell Niches in Human Foetal Hip, Knee and Elbow Cartilage Rudiments and Has Potential Roles in the Regulation of Stem Cell Differentiation. J Stem Cell Res Dev Ther 3: 009. AbstractThis study immunolocalized perlecan, Type I and II collagen and aggrecan in human foetal joint rudiment cartilages of the hip, knee and elbow. Blood vessels in the stromal tissues, associated with the cartilage rudiments were also prominently stained with perlecan, entrapped red blood cells aided in their identification. Perlecan was a prominent extracellular matrix proteoglycan in the rudiment cartilages. Stem cell niches close to the margins of the cartilage rudiments were prominently identified in the hip, knee and elbow and were distinguishable from the aforementioned blood vessels which were located in the rudiment associated stromal tissues. Type I collagen was also immunolocalized to the outer margins of the rudiment cartilages where perlecan positive niches were also present whereas the remainder of the rudiment contained type II collagen. Stem cell niches similar to those observed in the present study have previously been identified in the human foetal elbow.

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“…Cell migration is usually evaluated using wound healing assay or Transwell migration assay [17,28,30,33]. In the fi rst case, it is very diffi cult to create a gradient of chemotactic factors modulating migration, and in the second, cell migration cannot be measured in real time.…”

Section: Resultsmentioning

confidence: 99%

Functionally Active Gap Junctions between Connexin 43-Positive Mesenchymal Stem Cells and Glioma Cells

Габашвили

Baklaushev

Grinenko³

et al. 2015

Bull Exp Biol Med

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The formation of functional gap junctions between mesenchymal stem cells and cells of low-grade rat glioma C6 cells was studied in in vitro experiments. Immunocytochemical analysis with antibodies to connexin 43 extracellular loop 2 showed that mesenchymal stem cells as well as C6 glioma cells express the main astroglial gap junction protein connexin 43. Analysis of migration activity showed that mesenchymal stem cells actively migrate towards C6 glioma cells. During co-culturing, mesenchymal stem cells and glioma C6 form functionally active gap junctions mediating the transport of cytoplasmic dye from glioma cells to mesenchymal stem cells in the opposite direction. Fluorometry showed that the intensity of transport of low-molecular substances through heterologous gap junctions between mesenchymal stem cells and glioma cells is similar to that through homologous gap junctions between glioma cells. This phenomenon can be used for the development of new methods of cell therapy of high-grade gliomas.

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The Effect of Stromal Cell-Derived Factor 1 in the Migration of Neural Stem Cells

Cited by 30 publications

References 32 publications

SDF-1 overexpression by mesenchymal stem cells enhances GAP-43-positive axonal growth following spinal cord injury

SDF-1 overexpression by mesenchymal stem cells enhances GAP-43-positive axonal growth following spinal cord injury

Perlecan Delineates Stem Cell Niches in Human Foetal Hip, Knee and Elbow Cartilage Rudiments and Has Potential Roles in the Regulation of Stem Cell Differentiation

Functionally Active Gap Junctions between Connexin 43-Positive Mesenchymal Stem Cells and Glioma Cells

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