The effect of stromal vascular fraction in an experimental frostbite injury model

Oner, Cagatay; Irmak, Fatih; Eken, Gulcin; Oner, Burcu Bitir; Karşıdağ, Semra

doi:10.1016/j.burns.2022.02.011

Cited by 2 publications

(3 citation statements)

References 59 publications

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“…Furthermore, this group demonstrated increased granulation tissue, improved re-epithelialization, and heightened neovascularization. 25 In the current study, on the 7th day post-operation, the SVF-treated group observed a higher flap viability rate and vascular density compared to other groups. Aligned with existing literature, these results demonstrate the substantial contribution of SVF to wound healing.…”

Section: Discussionsupporting

confidence: 43%

Effect of adipose tissue-derived stromal vascular fraction on the viability of random-pattern skin flaps: an experimental study

Kul,

Özsoy

2024

ANKMJ

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Aims: Random pattern skin flaps are still widely used in plastic surgery. However, necrosis in distal flap sections resulting from ischemia is a serious problem, increasing the cost of treatment and hospitalization. The aim of this study was to test the effects of adipose tissue derived stromal vascular fraction (SVF) on random pattern skin flaps in rats. Methods: In this experimental study conducted on Wistar rats, three groups were formed using the simple random sampling technique. Group 1 consisted of rats with a raised flap and a subcutaneous SVF injection. Group 2 comprised rats that underwent a flap operation with no additional treatment. Group 3 included rats with a raised flap and a subcutaneous saline injection. Tissue necrosis and level of survival area was detected with planimetric method and histopathologic examination was performed for detect of level of vascular density. On the 7th day post-operation, the viability assessment was calculated based on the ratio of the living flap area to the total flap area. Results: The mean viability rate in Group 1 was higher compared to other groups, while there was no significant difference between Group 2 and Group 3 (Group 1 = 79.2 ± 4.9 % vs. Group 2 = 41.0 ± 5.9 % vs. Group 3= 39.2 ± 2.7 %, p < 0.001). The mean vascular densities of the flaps were higher in Group 1 compared to the other groups, while it were similar between Group 2 and Group 3 (Group 1 = 29.8 ± 1.2 vs. Group 2 = 8.4 ± 4.6 vs. Group 3= 9.3 ± 1.8, p < 0.001). Conclusion: The use of fat tissue-derived SVF injections has been found to be advantageous in improving the survival of random pattern flaps, frequently utilized in plastic and reconstructive surgery.

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Section: Discussionsupporting

confidence: 43%

Effect of adipose tissue-derived stromal vascular fraction on the viability of random-pattern skin flaps: an experimental study

Kul,

Özsoy

2024

ANKMJ

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“…The impact of different tissue source on the activity, composition, and post-preparation yield of SVF has been investigated. SVF in animal experiments is usually obtained from the groin [57], epididymis [32], etc. In clinical studies, fat is often obtained by liposuction or manual liposuction [58][59][60][61].…”

Section: Discussionmentioning

confidence: 99%

“…These cytokines can promote neovascularization. Ça gatay Öner et al [57] showed that SVF can also attenuate tissue fibrosis. Guerrero et al [74] found that T-cadherin-positive cells in SVF was found to promote the osteogenesis of SVF cells in vivo and strongly support the formation of vascular networks through the interaction of vascular endothelial growth factor and adiponectin.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Tissue Stromal Vascular Fraction as Compared to Cellular Stromal Vascular Fraction to Treat Anal Sphincter Incontinence

Chen

et al. 2022

Bioengineering

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Background: The long-term prognosis of current treatments for anal sphincter incontinence (ASI) is poor. Here, we explored the efficacy of tissue adipose stromal vascular fraction SVF (tSVF) on ASI and compared it to that of cellular SVF (cSVF). We then investigated possible mechanisms. Methods: Rat cSVF and tSVF were isolated and labeled with DIL. One day after modeling, three groups received phosphate-buffered saline (PBS), cSVF, tSVF, respectively. The control group received nil modeling nor any treatments. The effect was assessed by function test for anal pressure and electromyography, and staining for fiber content, proliferation and differentiation at day 5 and day 10. Results: cSVF injection resulted in faster healing than tSVF. The cSVF group showed significant improvement on anal pressure on day 10. For the electromyography test, cSVF showed significant improvement for the frequencies on day 10, and for the peak values on both time points, while tSVF showed significant improvement for the peak values on day 10. The two SVF both alleviated fibrosis. Immunofluorescence tracing identified differentiation of some injected cells towards myosatellite cells and smooth muscle cells in both SVF groups. For all the tests, the tSVF group tends to have similar or lower effects than the cSVF group with no significant difference. Conclusion: cSVF and tSVF are both safe and effective in treating ASI, while the effect of cSVF is slighter higher than tSVF.

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The effect of stromal vascular fraction in an experimental frostbite injury model

Cited by 2 publications

References 59 publications

Effect of adipose tissue-derived stromal vascular fraction on the viability of random-pattern skin flaps: an experimental study

Effect of adipose tissue-derived stromal vascular fraction on the viability of random-pattern skin flaps: an experimental study

The Effect of Tissue Stromal Vascular Fraction as Compared to Cellular Stromal Vascular Fraction to Treat Anal Sphincter Incontinence

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