The effect of substrate and enzyme concentration on the glucose syrup production from red sorghum starch by enzymatic hydrolysis

Permanasari, Ayu Ratna; Yulistiani, Fitria; Purnama, Rakhmadian; Widjaja, Tri; Gunawan, Setiyo

doi:10.1088/1755-1315/160/1/012002

Cited by 22 publications

(16 citation statements)

References 8 publications

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“…According to Chuck‐Hernandez et al ., the conversion of whole sorghum containing 727 g kg −1 starch (dry weight basis) in slurries containing 30% solids yielded 230 g L −1 glucose. On the other hand, Permanasari et al . converted whole sorghum with 530 g kg −1 starch and 40% solids in the slurry and obtained only 146 g L −1 glucose.…”

Section: Resultsmentioning

confidence: 85%

“…According to Chuck-Hernandez et al, 17 the conversion of whole sorghum containing 727 g kg −1 starch (dry weight basis) in slurries containing 30% solids yielded 230 g L −1 glucose. On the other hand, Permanasari et al 35 converted whole sorghum with 530 g kg −1 starch and 40% solids in the slurry and obtained only 146 g L −1 glucose. These contrasting results indicate that the original amount of starch and the solid concentration in the slurry play an important role during liquefaction and saccharification.…”

Section: Saccharificationmentioning

confidence: 99%

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Whole unripe plantain (Musa paradisiaca L.) as raw material for bioethanol production

et al. 2019

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BACKGROUND The use of byproducts such as rejected plantain with final disposition problems and conversion processes with ‘green’ technologies are important research topics. Bioethanol production from crops with a high content of fermentable sugars is an alternative to that from traditional crops (corn and sugar cane). The aim of this work was to study the use of whole (peel and pulp) unripe plantain (WP) for bioethanol production. RESULTS Lab‐scale liquefaction and saccharification of both materials released mainly three carbohydrates, glucose (9.02 mg g−1), maltose (0.45 mg g−1) and xylose (0.25 mg g−1). The WP saccharification required the use of pectinase and cellulase because of the high amounts of pectin and cellulose associated with the peel. Fermentation for 11 h produced similar ethanol concentration for both samples, but at the end of fermentation (32 h), the ethanol production was higher in the WP (58.6 mL L−1) compared with the plantain pulp (PP) (45.5 mL L−1). The theoretical ethanol yield was lower with WP (67%) than with PP (90%). CONCLUSION WP can be an alternative raw material for bioethanol production. © 2019 Society of Chemical Industry

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Section: Resultsmentioning

confidence: 85%

Section: Saccharificationmentioning

confidence: 99%

Whole unripe plantain (Musa paradisiaca L.) as raw material for bioethanol production

et al. 2019

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“…The effect of substrate concentration on the liquefaction of gelatinized Abrus seed slurry showed that the released reducing sugar increases from 2.92 to 5.44 mg/mL as Abrus seed flour concentration increases from 1.0 to 5.0% w/v, and decreases as substrate concentration increases from 10.0 to 30.0%w/v (Figure 3). This is because of increase in viscosity, which affects alpha‐amylase activity 53 . It has been observed that, as the substrate concentration keeps increasing, the yield of reducing sugar will decrease due to high viscosity of starch solution that resulted in the poor mixing of samples, which lower the activities of alpha‐amylase and glucoamylase 54 .…”

Section: Resultsmentioning

confidence: 99%

Optimization and characterization of bioethanol production from Abrus seed flour

2020

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Summary Development of alternative energy sources, that is, renewable, environmental co‐friendly, and cost effective, is highly imperative to mitigate the harmful effects associated with the use of fossil fuels. Thus, Abrus precatorius seeds are commercially unexploited in the production of bioethanol in Nigeria. The seed was found to contain an appreciable amount of carbohydrates (ca 61%), which are the main source of ethanol. Its lignin content (1.23%) was very low compared to its hydrolyzable components of lignocellulose‐cellulose (13.67%) and hemicellulose (12.53%), making the seed a potential source of bioethanol. The Abrus hydrolysate produced by liquefaction and saccharification, using partially purified α‐amylase and glucoamylase, respectively, had a reducing sugar content of 10.90 mg/mL. Studies on the liquefaction of Abrus seed slurry showed that a maximum reducing sugar of 5.32 mg/mL was released at optimal conditions of substrate concentration (5 g/100 mL), pH 5.0, temperature 60°C, α‐amylase concentration 50 U/mL, and incubation period of 80 minutes, while the saccharification of liquefied slurry released a maximum reducing sugar of 10.90 mg/mL at optimal conditions of pH 5.5, temperature 60°C, glucoamylase concentration 40 U/mL, and incubation period of 90 minutes. The optimization of bioethanol production from Abrus hydrolysate showed that a maximum bioethanol yield of 90.57 ± 0.24% was obtained at an optimal incubation period of 5 days, inoculum size of 5% w/v, and pH of 5.5. The fuel properties of the Abrus bioethanol compared favorably well with standard ethanol, especially in the aspect of heat content (28 580.77 ± 0.05 KJ/Kg), kinematic viscosity at 40°C (1.30 ± 0.02 mm2/s), refractive index (1.36 ± 0.27), and pour point (−18.00 ± 0.07°C). Ash and sulfur were not detected in the produced bioethanol. This study showed that, under normal bioethanol production conditions, inedible Abrus seed flour can serve as cheap and effective feedstock for industrial production of bioethanol. Novelty Statement The depletion of the global reserves of fossil fuels and the increasing environmental concerns due to fossil fuel usage had spurred global interests in renewable alternative energy sources. Most of the carbon‐neutral alternative sources are edible crops, raising concern about food security in the ever‐increasing global population. Here, a good quality bioenergy is developed from a nonfood plant source, Abrus precatorius seed, to mitigate the negative effects of fossil fuel consumption on the environment without interfering with the global food chain.

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“…The production rate of histamine is affected by the free-histidine availability in the medium [21]. The higher the substrate concentration, the higher the product is produced, the histidine concentration as the substrate will affect the amount of histamine formed [19]. This explained the abundance of histamine found in Scromboidae fishes is due to their high content of free histidine in the muscle tissues [12].…”

Section: Fig 3 Visualization Of Histidine (A) and Histamine Standarmentioning

confidence: 99%

Growth Rate and Histamine Production of Citrobacter freundii CK01 in Various Incubation Temperatures

2020

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Improper handling and temperature fluctuations during postharvest of fish commonly allow the growth of histamine-forming bacteria (HFB) that may cause histamine poisoning. Citrobacter freundii CK01 is one of the HFB isolated from Skipjack landed on Sadeng, Yogyakarta. This study aimed to determine the temperature effect on the growth rate and histamine production by C. freundii CK01. Bacterial growth and histamine production was tested in tuna fish infusion broth (TFIB) at 5, 15, 30 and 40°C. The bacterial growth was tested using Total Plate Count method, while histamine was determined using Thin Layer Chromatography and densitometry method. The primary model for bacterial growth was plotted with incubation time using DMFit referred to Baranyi & Roberts model. The secondary model was converted from growth rate and modeled using Ratkowsky Square Root Model. The equation for growth rate of C. freundii CK01 was μmax=[0.0105 (T + 13.886)] 2 (Root Mean Square Error <10%). Histamine production reached the highest concentration at 15°C in 96 hours up to 117,13 ppm. This study shows that temperature affected the growth rate and histamine production of C. freundii, indicating the importance of maintaining the low temperature stability during handling of skipjack.

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The effect of substrate and enzyme concentration on the glucose syrup production from red sorghum starch by enzymatic hydrolysis

Cited by 22 publications

References 8 publications

Whole unripe plantain (Musa paradisiaca L.) as raw material for bioethanol production

Whole unripe plantain (Musa paradisiaca L.) as raw material for bioethanol production

Optimization and characterization of bioethanol production from Abrus seed flour

Growth Rate and Histamine Production of Citrobacter freundii CK01 in Various Incubation Temperatures

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