The effect of supplementation of cryopreservation diluents with sugars on the post-thawing fertility of ram semen

Jafaroghli, M.; Khalili, B.; Farshad, Abbas; Zamiri, Mohammad Javad

doi:10.1016/j.smallrumres.2010.11.010

Cited by 52 publications

(32 citation statements)

References 31 publications

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“…Najafi et al (2013) demonstrated that in a soybean lecithin-based extender, the combination of 5% glycerol and 100 mM trehalose resulted in higher post-thawing sperm quality (motility, viability, morphology, acrosome and plasma membrane integrity) than other combinations, or using glycerol alone, indicating a synergistic effect. These findings agree with Jafaroghli et al (2011), who obtained the highest post-thawing semen quality by the combination of 5% glycerol with 100 mM trehalose in a Tris-citric acid-fructoseyolk extender. Similar findings were provided by Tonieto et al (2010) by the supplementation of Trisegg yolk-glycerol extender with100 mM trehalose and 8% LDL, while Matsuoka et al (2006) noticed that the addition of trehalose to an egg-yolk based ram semen diluent containing fructose, improves post-thaw sperm motility and viability.…”

Section: ) Research Concerning "Sugars" Effects and Propertiessupporting

confidence: 92%

“…Similar findings were provided by Tonieto et al (2010) by the supplementation of Trisegg yolk-glycerol extender with100 mM trehalose and 8% LDL, while Matsuoka et al (2006) noticed that the addition of trehalose to an egg-yolk based ram semen diluent containing fructose, improves post-thaw sperm motility and viability. Although trehalose is the most investigated sugar additive in semen extenders, other sugars such as sucrose, raffinose and hypotaurine also resulted in higher ram semen cryoprotection (Bucak et al, 2013;Jafaroghli et al, 2011). Furthermore, a beneficial role of cyclodextrins, a family of cyclic oligosaccharides with a hydrophilic outer surface and a lipophilic central cavity, on semen has been found by Mocé et al (2010).…”

Section: ) Research Concerning "Sugars" Effects and Propertiesmentioning

confidence: 99%

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Current status and advances in ram semen cryopreservation

Ntemka

Tsakmakidis

Kiossis

et al. 2018

J Hellenic Vet Med Soc

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Ram semen cryopreservation contributes to genetic improvement through artificial insemination, eliminates geographical barriers in artificial insemination application and supports the preservation of endangered breeds thus the conservation of biodiversity. Sperm freezing process induces ultrastructural, biochemical and functional changes of spermatozoa. Especially, spermatozoa’s membranes and chromatin can be damaged, sperm membranes’ permeability is increased, hyper oxidation and formation of reactive oxygen species takes place, affecting fertilizing ability and subsequent early embryonic development. Aiming to improve ram frozen-thawed semen’s fertilizing capacity, many scientific investigations took place. Among them the composition of semen extenders, was a main point of interest. Semen preservation extenders regulate and support an environment of adequate pH and buffering capacity to protect spermatozoa from osmotic and cryogenic stress. Therefore, permeating (glycerol, dimethyl sulfoxide) and non-permeat ing (egg yolk, skimmed milk) cryoprotectants, sugars (glucose, lactose, trehalose, raffinose), salts (sodium citrate, citric acid) and antioxidants (amino acids, vitamins, enzymes) have been added and tested. Moreover, semen dilution rate, storage temperature, cooling rate and thawing protocol, are also some key factors that have been studied. The research results of this scientific topic are encouraging, not only about the freezing and thawing procedures, but also about the improvement of the additives’ properties. However, further research is needed to enhance the fertilizing ability of ram frozen-thawed semen, making its use practical in sheep reproductive management by the application of cervical artificial insemination.

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Section: ) Research Concerning "Sugars" Effects and Propertiessupporting

confidence: 92%

Section: ) Research Concerning "Sugars" Effects and Propertiesmentioning

confidence: 99%

Current status and advances in ram semen cryopreservation

Ntemka

Tsakmakidis

Kiossis

et al. 2018

J Hellenic Vet Med Soc

View full text Add to dashboard Cite

show abstract

“…Also, owing to the fact that it is a non-permeable disaccharide, trehalose could protect cells during the cooling procedure when spermatozoa lose water (Aisen et al 2005). Aisen et al (2002) reported that trehalose not only renders the membrane stable, but also prevents uncontrolled ROS production and makes the extender more stable (Jafaroghli et al 2011). Trehalose exerted significant positive effects with respect to membrane protection and sperm viability rates in comparison to the control group in this study.…”

Section: Discussionmentioning

confidence: 58%

“…Each pooled ejaculate was split into five equal aliquots and diluted at 37 °C with base extenders containing trehalose (10 and 25 mM), cysteine (2 and 4 mM) or no antioxidant (control), respectively. Specific trehalose and cysteine concentrations were selected based on results obtained from a previous trehalose and cysteine standardisation study with ram and bull semen (Aboagla and Terada 2003;Sariozkan et al 2009;Hu et al 2010;Coyan et al 2011;Jafaroghli et al 2011). The final semen concentration was 4 × 10 8 sperm/ml (single step dilution), in 15-ml polypropylene centrifuge tubes.…”

Section: Methodsmentioning

confidence: 99%

Positive effects of trehalose and cysteine on ram sperm parameters

Güngör¹,

Öztürk²,

Omur³

2017

Vet. Med. - Czech

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ABSTRACT:The aim of this study was to determine the effects of trehalose and cysteine on sperm motility, viability, mitochondrial activity and acrosome integrity during liquid storage of Merino ram semen. Ejaculates were collected using artificial vaginas from five Merino rams, microscopically evaluated and pooled at 37 °C. The pooled semen samples were diluted in a Tris-based extender, including cysteine (2 mM and 4 mM), trehalose (10 mM and 25 mM) and no antioxidant (control). Diluted semen samples were kept in tubes and cooled from 37 to 5 °C in a cold cabinet, and maintained at 5 °C. Cooled samples were evaluated for sperm motility, viability, mitochondrial activity and acrosome integrity at 0, 24, 48, 72 and 96 h. Extender supplemented with trehalose (10 and 25 mM) and cysteine (2 and 4 mM) led to higher motility in comparison to the control at 24, 48, 72 and 96 h of liquid storage (P < 0.05). Trehalose at the doses of 10 mM, 25 mM and 2 mM cysteine led to higher viability between 24-48-72 h and at 96 h of liquid storage (P < 0.05). Further, 4 mM of cysteine improved sperm viability rates at 24 and 48 h of storage compared to the control group (P < 0.05), and resulted in improved acrosome integrity rates compared to the control group at 72 and 96 h of storage (P < 0.05). Extender supplemented with 10 and 25 mM trehalose at 24 and 72 h and 4 mM cysteine at 24 and 96 h of storage led to higher sperm mitochondrial activity rates when compared to the control group (P < 0.05). In conclusion, the findings of this study show that trehalose and cysteine provided significant protection to ram sperm parameters during liquid storage.

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“…Viable sperm related with membrane integrity. In most studies revealed that trehalose supplementation in semen extender enhances the membrane integrity of sperm in farm animals (Hu et al, 2009;Khalili et al, 2009;Tonieto et al, 2010;Jafaroghli et al, 2011). The presence of trehalose in extenders is likely to modulate membrane fluidity by inserting itself into membrane phospholipids bilayer, thus it renders membrane more stable during cooling.…”

Section: The Sperm Survival Of Stallion Chilled Semenmentioning

confidence: 99%

The Quality of Stallion Semen in Skim Milk and Dimitropoulos Extenders Preserved at 5 oC and Ambient Temperature Supplemented with Different Sugar

Arifiantini¹,

Purwantara²,

Yusuf³

et al. 2013

med pet

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This study was conducted to evaluate the effects of sugars supplementation in skim milk based (SM) and dimitropoulos (DV) extenders on the sperm motility and viability in stallion semen storage at 5 o C and ambient temperature (24-29 o C). Semen samples were collected from 3 stallions; evaluate individually and each of them divided into 8 aliquots. Four out of eight aliquots were diluted 1:1 with SM, while the remaining four were diluted 1:1 with DV; all were then centrifuged at 1006 g (3000 RPM) for 15 min. The supernatants were discarded, and each pellet was re-diluted with SM (control), SM trehalose (SMT), SM-raffinose (SMR), SM-fructose (SMF), DV (control), DV-trehalose (DVT), DV-raffinose (DVR), and DV-fructose (DVF). The diluted semen were divided into 2 aliquots and stored at 5 o C or ambient temperature. The sperm motility and viability were evaluated every 3 h on chilled semen stored at ambient temperature, and every 12 h on those stored at 5 o C. Results of the experiments demonstrated that sperm motility and viability in DV extender significantly higher (P<0.05) in both temperature. The supplementation of fructose was the best on the motility and viability of the sperm at both temperatures compare to trehalose, raffinose, or the control group. The best extender and sugar combination was DVF, which the total motile sperm stored at 5 o C for 96 h was 45.1% followed by to DVT (40.2%) and DVR (39.2%). The sperm motility in DV and SMF were 35.3% and 35.6%, respectively; these were higher than those diluted with control (28.9%); SMT (30.3%), and SMR (29.6%). The study concluded that the supplementation of fructose in DV extender (DVF) was the best combination to preserve stallion sperm motility and viability stored at 5 o C or ambient temperature.

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The effect of supplementation of cryopreservation diluents with sugars on the post-thawing fertility of ram semen

Cited by 52 publications

References 31 publications

Current status and advances in ram semen cryopreservation

Current status and advances in ram semen cryopreservation

Positive effects of trehalose and cysteine on ram sperm parameters

The Quality of Stallion Semen in Skim Milk and Dimitropoulos Extenders Preserved at 5 oC and Ambient Temperature Supplemented with Different Sugar

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