The effect of surface charge on the uptake and biological function of mesoporous silica nanoparticles in 3T3-L1 cells and human mesenchymal stem cells

Chung, Tsai Hua; Wu, Si Han; Yao, Ming; Lu, Chen Wen; Yu, Lin; Hung, Yu-Chueh; Mou, Chung‐Yuan; Chen, Yao Chang; Dong, Hao

doi:10.1016/j.biomaterials.2007.03.006

Cited by 573 publications

(415 citation statements)

References 24 publications

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“…For example, ion leaching can either be detected in cell-free conditions using specialized buffers (pH 7.4; 5.5 and 4.5) and the intactness of stem cell functionality can be evaluated by observing the efficiency of cellular differentiation induced by specific protocols 40,92 .…”

Section: A Routine In Vitro Methodsmentioning

confidence: 99%

Assessing nanoparticle toxicity in cell-based assays: influence of cell culture parameters and optimized models for bridging the in vitro–in vivo gap

et al. 2013

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Section: A Routine In Vitro Methodsmentioning

confidence: 99%

Assessing nanoparticle toxicity in cell-based assays: influence of cell culture parameters and optimized models for bridging the in vitro–in vivo gap

et al. 2013

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“…The biocompatibility of MSNs and several carbon-based biomaterials (e.g., carbon nanotubes and graphene) have been systematically investigated using several metrics, including biodistribution, excretion, biodegradation, histocompatibility, and hemocompatibility [102][103][104][105][106][107]. They have been demonstrated to be non-toxic at desirable doses.…”

Section: Biocompatibility Of Mcbsmentioning

confidence: 99%

Mesoporous carbon biomaterials

Chen

Shi

2015

Sci. China Mater.

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Nano-biotechnology provides highly efficient and versatile strategies to improve the diagnostic precision and therapeutic efficiency of serious diseases. The development of new biomaterial systems provides great opportunities for the successful clinical translation of nano-biotechnology for personalized biomedicine to benefit patients. As a new inorganic material system, mesoporous carbon biomaterials (

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“…This principle has been described for a variety of nanoparticle (examples include gold [39], polymer [40,41] and silica [42]) and biological (for example delivery of DNA with cationic proteins, lipids and polymers [43]) contexts. The aminated surfaces of the particles used in this study provide an inherent surface charge, facilitating cellular interaction.…”

Section: Cell Loadingmentioning

confidence: 99%

Size, charge and concentration dependent uptake of iron oxide particles by non-phagocytic cells

2008

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A promising new direction for contrast-enhanced magnetic resonance (MR) imaging involves tracking the migration and biodistribution of superparamagnetic iron oxide (SPIO)-labeled cells in vivo. Despite the large number of cell labeling studies that have been performed with SPIO particles of differing size and surface charge, it remains unclear which SPIO configuration provides optimal contrast in non-phagocytic cells. This is largely because contradictory findings have stemmed from the variability and imprecise control over surface charge, the general need and complexity of transfection and/or targeting agents, and the limited number of particle configurations examined in any given study. In the present study, we systematically evaluated the cellular uptake of SPIO in non-phagocytic T cells over a continuum of particle sizes ranging from 33 nm to nearly 1.5 μm, with precisely controlled surface properties, and without the need for transfection agents. SPIO labeling of T cells was analyzed by flow cytometry and contrast enhancement was determined by relaxometry. SPIO uptake was dose dependent and exhibited sigmoidal charge dependence, which was shown to saturate at different levels of functionalization. Efficient labeling of cells was observed for particles up to 300nm, however micron-sized particle uptake was limited. Our results show that an unconventional highly cationic particle configuration at 107 nm maximized MR contrast of T cells, outperforming the widely utilized USPIO (<50 nm).

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The effect of surface charge on the uptake and biological function of mesoporous silica nanoparticles in 3T3-L1 cells and human mesenchymal stem cells

Cited by 573 publications

References 24 publications

Assessing nanoparticle toxicity in cell-based assays: influence of cell culture parameters and optimized models for bridging the in vitro–in vivo gap

Assessing nanoparticle toxicity in cell-based assays: influence of cell culture parameters and optimized models for bridging the in vitro–in vivo gap

Mesoporous carbon biomaterials

Size, charge and concentration dependent uptake of iron oxide particles by non-phagocytic cells

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