The effect of surface immobilized NBD peptide on osteoclastogenesis of rough titanium plates <i>in vitro</i> and osseointegration of rough titanium implants in ovariectomized rats <i>in vivo</i>

Wang, Yu; Zhang, Chen; Xu, Weijian; Wang, Baixiang; Lan, Yanhua; Yu, Mengfei; Wang, Pinger; Xie, Zhijian

doi:10.1039/c8ra03116a

Cited by 3 publications

(2 citation statements)

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“…The adhesion of rBMSCs and the physical location and interactions between the cells and exosomes on the Ti surface were evaluated using a field-emission scanning electron microscope (FE-SEM; SU8010, Hitachi, Tokyo, Japan). In summary, the acid-etched Ti plates loaded with cells and exosome samples were fixed in 2% paraformaldehyde at 4 • C for 15 min, washed with 0.15 M sodium cocoate buffer, and stained with 1% osmium tetraoxide for 2 h. After rinsing with the buffer, the samples were dehydrated through an ethanol series (30 min each in 50, 60, 70, 80, 90, 95, 100%, and dry ethanol) and dried in the air before gold sputter coating and observation (Wang Y. et al, 2018).…”

Section: Observation Of Adherent Cells On Ti Plates Using Scanning Elmentioning

confidence: 99%

“…However, the use of these traditional biotic implant modifications has several disadvantages, such as explosive release, immunogenicity, instability, and easy degradation. Recently, it has been proven that nanoscale materials that show novel physical, chemical, and biological properties with high stability could be efficient alternatives to increased cell responses, bone conduction, and osseointegration (Wang Y. et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Exosomes Enhance Adhesion and Osteogenic Differentiation of Initial Bone Marrow Stem Cells on Titanium Surfaces

Lan

Jin

Xie

et al. 2020

Front. Cell Dev. Biol.

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Successful osseointegration involves the biological behavior of bone marrow stem cells (BMSCs) on an implant surface; however, the role of BMSC-derived extracellular vesicles (EVs)/exosomes in osseointegration is little known. This study aimed to: (i) explore the interaction force between exosomes (Exo) and cells on a titanium surface; (ii) discuss whether the morphology and biological behavior of BMSCs are affected by exosomes; and (iii) preliminarily investigate the mechanism by which exosomes regulate cells on Ti surface. Exosomes secreted by rat BMSCs were collected by ultracentrifugation and analyzed using transmission electron microscopy and nanoparticle tracking analysis. Confocal fluorescence microscopy, scanning electron microscopy, Cell Counting Kit-8 (CCK-8), quantitative real-time polymerase chain reaction techniques, and alkaline phosphatase bioactivity, Alizarin Red staining, and quantification were used to investigate the exosomes that adhere to the Ti plates under different treatments as well as the morphological change, adhesion, spread, and differentiation of BMSCs. We found that exosomes were efficiently internalized and could regulate cell morphology and promoted the adhesion, spreading, and osteogenic differentiation of BMSCs. These were achieved partly by activating the RhoA/ROCK signaling pathway. Our discovery presents a new insight into the positive regulatory effect of exosomes on the biological behaviors of BMSCs on Ti surface and provides a novel route to modify the surface of a Ti implant.

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Section: Observation Of Adherent Cells On Ti Plates Using Scanning Elmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%