Coating of the metal surface for different fields such as corrosion resistance, hydrophobic surface, and conductivity properties enhance the material quality for different application. Furthermore, controlling the morphological structures and the size of the coating materials is one of the most important factors for designing their functionality. The new approaches to the effect of surface properties at the atomic level on bacterial adhesion offer new perspectives on this subject. In this study, the effect of the different current densities on the morphological microstructural features, micro hardness properties, and bacterial attachment behavior of the protective aluminum oxide layer was investigated by using different analytical and instrumental methods. The Staphylococcus aureus was used as a model bacteria for attachment tests. The result presented in the current study exhibited that the crystal morphology, hardness, and bacterial adhesion features have changed depending on the applied current density. The higher current density increases the surface roughness, hardness, and the size of the surface morphology and develops the bacterial attachment on the surface.