The effect of TAT conjugated platinum nanoparticles on lifespan in a nematode Caenorhabditis elegans model

Kim, Juewon; Shirasawa, Takuji; Miyamoto, Yusei

doi:10.1016/j.biomaterials.2010.03.077

Cited by 76 publications

(51 citation statements)

References 28 publications

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“…With reference to the researches of Kim et al, 38) the synthesized Pt NPs internalized into nematodes might have been less than 1 ppb in our research. The recent epigenetic oxidative redox shift theory of aging suggests that moderate oxidative stress extends the lifespans of animals, while both excess and insufficient oxidative stress result in shortening of lifespan via mitochondrial dysfunction.…”

Section: Discussionmentioning

confidence: 94%

Mechanism of the Lifespan Extension ofCaenorhabditis elegansby Electrolyzed Reduced Water—Participation of Pt Nanoparticles

Yan

Kinjo

Tian

et al. 2011

Bioscience, Biotechnology, and Biochemistry

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Electrolyzed reduced water (ERW) contains a large amount of molecular hydrogen and a small amount of Pt nanoparticles (Pt NPs). We have found that ERW significantly extended the lifespan of Caenorhabditis elegans in a novel culture medium designated Water Medium. In this study, we found that synthetic Pt NPs at ppb levels significantly extended the nematode lifespan and scavenged reactive oxygen species (ROS) in the nematode induced by paraquat treatment. In contrast, a high concentration of dissolved molecular hydrogen had no significant effect on the lifespan of the nematode. These findings suggest that the Pt NPs in ERW, rather than the molecular hydrogen, extend the longevity of the nematode, at least partly by scavenging ROS.

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Section: Discussionmentioning

confidence: 94%

Mechanism of the Lifespan Extension ofCaenorhabditis elegansby Electrolyzed Reduced Water—Participation of Pt Nanoparticles

Yan

Kinjo

Tian

et al. 2011

Bioscience, Biotechnology, and Biochemistry

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“…that used S Medium, the authors could conclude that a NP concentration of 1.0 ppb would have been internalized into the nematodes. Since this amount is so small, they conclude that it is highly likely that drinking ERAW does not have any side effects [26]. This explains why toxicology tests have always been negative so far and thus ERAW declared safe to drink [29].…”

Section: Anti-oxidant Activity Of Eraw Contaminated By Nps During Thementioning

confidence: 99%

“…An increase in life span of nematodes caused by treatment with salen-manganese that stimulates superoxide dismutase and catalase is further reinforced by the presence of NPs [25]. By fixing the NPs to a peptide sequence that has high affinity for platinum and further linked to a peptide sequence derived from HIV-1TAT and allowing it to pass through the cellular membrane and penetrate cells, the cellular internalization of NPs was increased and the effective dose producing the same effects was now 5 µM, which is 100 times lower [26].…”

Section: Study Of the Anti-oxidizing Action Of Synthetic Nps Supsensionsmentioning

confidence: 99%

Physico-Chemical, Biological and Therapeutic Characteristics of Electrolyzed Reduced Alkaline Water (ERAW)

Henry

Chambron

2013

Water

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Abstract:The consumption of alkaline reduced water produced by domestic electrolysis devices was approved in Japan in 1965 by the Ministry of Health, Labour and Welfare for the cure of gastro-intestinal disorders. Today, these devices are freely available in several countries and can be easily purchased without reserve. The commercial information included with the device recommends the consumption of 1-1.5 L of water per day, not only for gastro-intestinal disorders but also for numerous other illnesses such as diabetes, cancer, inflammation, etc. Academic research in Japan on this subject has been undergoing since 1990 only but has established that the active ingredient is dissolved dihydrogen that eliminates the free radical HO• in vivo. In addition, it was demonstrated that degradation of the electrodes during functioning of the device releases very reactive nanoparticles of platinum, the toxicity of which has not yet been clearly proven. This report recommends alerting health authorities of the uncontrolled availability of these devices used as health products, but which generate drug substances and should therefore be sold according to regulatory requirements.

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“…Although other antioxidants can only scavenge specific types of reactive oxygen, nano-Pt can function as reductive catalysts to scavenge all types of ROS. It has been suggested that nano-Pt scavenge ROS by functioning as a superoxide dismutase (SOD) / catalase mimetic (18,19). However, although there have been several studies regarding the biological effects of nano-Pt, it is unknown whether nano-Pt have a direct effect on osteoclast differentiation.…”

Section: Introductionmentioning

confidence: 99%

Platinum Nanoparticles Suppress Osteoclastogenesis Through Scavenging of Reactive Oxygen Species Produced in RAW264.7 Cells

et al. 2011

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Abstract.Recent research has shown that platinum nanoparticles (nano-Pt) efficiently quench reactive oxygen species (ROS) as a reducing catalyst. ROS have been suggested to regulate receptor activator of NF-κB ligand (RANKL)-stimulated osteoclast differentiation. In the present study, we examined the direct effects of platinum nano-Pt on RANKL-induced osteoclast differentiation of murine pre-osteoclastic RAW 264.7 cells. The effect of the nano-Pt on the number of osteoclasts was measured and their effect on the mRNA expression for osteoclast differentiation was assayed using real-time PCR. Nano-Pt appeared to have a ROS-scavenging activity. Nano-Pt decreased the number of osteoclasts (2+ nuclei) and large osteoclasts (8+ nuclei) in a dose-dependent manner without affecting cell viability. In addition, this agent significantly blocked RANKL-induced mRNA expression of osteoclastic differentiation genes such as c-fms, NFATc1, NFATc2, and DC-STAMP as well as that of osteoclast-specific marker genes including MMP-9, Cath-K, CLC7, ATP6i, CTR, and TRAP. Although nano-Pt attenuated expression of the ROS-producing NOXfamily oxidases, Nox1 and Nox4, they up-regulated expression of Nox2, the major Nox enzyme in macrophages. These findings suggest that the nano-Pt inhibit RANKL-stimulated osteoclast differentiation via their ROS scavenging property. The use of nano-Pt as scavengers of ROS that is generated by RANKL may be a novel and innovative therapy for bone diseases.

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The effect of TAT conjugated platinum nanoparticles on lifespan in a nematode Caenorhabditis elegans model

Cited by 76 publications

References 28 publications

Mechanism of the Lifespan Extension ofCaenorhabditis elegansby Electrolyzed Reduced Water—Participation of Pt Nanoparticles

Mechanism of the Lifespan Extension ofCaenorhabditis elegansby Electrolyzed Reduced Water—Participation of Pt Nanoparticles

Physico-Chemical, Biological and Therapeutic Characteristics of Electrolyzed Reduced Alkaline Water (ERAW)

Platinum Nanoparticles Suppress Osteoclastogenesis Through Scavenging of Reactive Oxygen Species Produced in RAW264.7 Cells

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