The effect of temperature adaptation on the ubiquitin-proteasome pathway in notothenioid fishes

Todgham, Anne E.; Crombie, Timothy A.; Hofmann, Gretchen E.

doi:10.1242/jeb.145946

Cited by 53 publications

(35 citation statements)

References 74 publications

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“…7C). This indicates incomplete warm compensation of protein turnover as both protein synthesis and degradation may increase with temperature (Storch et al, 2003(Storch et al, , 2005Todgham et al, 2017;Whiteley et al, 1997). In agreement with this, cold acclimation of cardiac performance was observed in adult M. magister (Prentice and Schneider, 1979), whereas there was no evidence for seasonal (warm) acclimatization in juveniles (McLean and Todgham, 2015).…”

Section: Discussionsupporting

confidence: 55%

Effects of temperature on juvenile Dungeness crab,Metacarcinus magister(Dana): survival, moulting, and mTOR signalling and neuropeptide gene expression in eyestalk ganglia, moulting gland (Y-organ), and heart

Wittmann

Benrabaa

López-Cerón

et al. 2018

Journal of Experimental Biology

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Mechanistic target of rapamymcin (mTOR) is a highly conserved protein kinase that controls cellular protein synthesis and energy homeostasis. We hypothesize that mTOR integrates intrinsic signals (moulting hormones) and extrinsic signals (thermal stress) to regulate moulting and growth in decapod crustaceans. The effects of temperature on survival, moulting and mRNA levels of mTOR signalling genes (, ,, and) and neuropeptides ( and ) were quantified in juvenile Crabs at different moult stages (12, 19 or 26 days postmoult) were transferred from ambient temperature (∼15°C) to temperatures between 5 and 30°C for up to 14 days. Survival was 97-100% from 5 to 20°C, but none survived at 25 or 30°C. Moult stage progression accelerated from 5 to 15°C, but did not accelerate further at 20°C. In eyestalk ganglia, , and mRNA levels decreased with increasing temperatures. and mRNA levels were lowest in the eyestalk ganglia of mid-premoult animals at 20°C. In the Y-organ, mRNA levels decreased with increasing temperature and increased during premoult, and were positively correlated with haemolymph ecdysteroid titre. In the heart, moult stage had no effect on mTOR signalling gene mRNA levels; only , and mRNA levels were higher in intermoult animals at 10°C. These data suggest that temperature compensation of neuropeptide and mTOR signalling gene expression in the eyestalk ganglia and Y-organ contributes to regulate moulting in the 10 to 20°C range. The limited warm compensation in the heart may contribute to mortality at temperatures above 20°C.

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Section: Discussionsupporting

confidence: 55%

Effects of temperature on juvenile Dungeness crab,Metacarcinus magister(Dana): survival, moulting, and mTOR signalling and neuropeptide gene expression in eyestalk ganglia, moulting gland (Y-organ), and heart

Wittmann

Benrabaa

López-Cerón

et al. 2018

Journal of Experimental Biology

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“…The conjugation of ubiquitin with target proteins promotes binding to the proteasome and subsequent protein degradation. Protein ubiquitination is transcriptionally enhanced in cold-exposed fish [48,56,57], and frogs [55], possibly due to cold-induced denaturation [57], and may serve as a marker for cold exposure [48]. Protein turnover in cold D. chrysoscelis could supply the cell with amino acids to be used for synthesis of ATP, carbohydrates, and proteins, or these might serve as cryoprotectants [2] or as sources of cryoprotective urea [15].…”

Section: Differential Expression Of Genes Involved In Protein Amino mentioning

confidence: 99%

Hepatic transcriptome of the freeze-tolerant Cope’s gray treefrog, Dryophytes chrysoscelis: responses to cold acclimation and freezing

et al. 2020

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Background: Cope's gray treefrog, Dryophytes chrysoscelis, withstands the physiological challenges of corporeal freezing, partly by accumulating cryoprotective compounds of hepatic origin, including glycerol, urea, and glucose. We hypothesized that expression of genes related to cryoprotectant mobilization and stress tolerance would be differentially regulated in response to cold. Using high-throughput RNA sequencing (RNA-Seq), a hepatic transcriptome was generated for D. chrysoscelis, and gene expression was compared among frogs that were warmacclimated, cold-acclimated, and frozen. Results: A total of 159,556 transcripts were generated; 39% showed homology with known transcripts, and 34% of all transcripts were annotated. Gene-level analyses identified 34,936 genes, 85% of which were annotated. Cold acclimation induced differential expression both of genes and non-coding transcripts; freezing induced few additional changes. Transcript-level analysis followed by gene-level aggregation revealed 3582 differentially expressed genes, whereas analysis at the gene level revealed 1324 differentially regulated genes. Approximately 3.6% of differentially expressed sequences were non-coding and of no identifiable homology. Expression of several genes associated with cryoprotectant accumulation was altered during cold acclimation. Of note, glycerol kinase expression decreased with cold exposure, possibly promoting accumulation of glycerol, whereas glucose export was transcriptionally promoted by upregulation of glucose-6-phosphatase and downregulation of genes of various glycolytic enzymes. Several genes related to heat shock protein response, DNA repair, and the ubiquitin proteasome pathway were upregulated in cold and frozen frogs, whereas genes involved in responses to oxidative stress and anoxia, both potential sources of cellular damage during freezing, were downregulated or unchanged.

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“…There have been many studies of physiological capacities to respond to temperature stress in Antarctic marine species over recent decades on a very wide range of different taxa, including fish (e.g. Macdonald & Montgomery 1982, Hardewig et al 1999b, Hofmann et al 2000, Wilson et al 2001, Podrabsky & Somero 2006, Franklin et al 2007, Robinson & Davison 2008, Bilyk & DeVries 2011, Strobel et al 2012, Todgham et al 2017, molluscs (Peck 1989, Urban & Silva 1998, Clark et al 2008a,b, Morley et al 2010,b,c, Reed et al 2012, Reed & Thatje 2015, echinoderms (Stanwell-Smith & Peck 1998, Clark et al 2008b, Morley et al 2012b, amphipods (Young et al 2006a,b, Clark et al 2008b, Doyle et al 2012, Gomes et al 2013, Schram et al 2015b, isopods (Whiteley et al 1996, Robertson et al 2001, Young et al 2006a,b, Janecki et al 2010, brachiopods (Peck 1989, Peck et al 1997a, sponges (Fillinger et al 2013), and macroalgae or phytoplankton (Montes-Hugo et al 2009, Schloss et al 2012. There have also been many assessments of the effects of elevated temperature using a larger-scale approach, both experimentally and using field observations identifying multispecies response or evaluating community, ecosystem or overall biodiversity level responses (e.g.…”

Section: Rising Temperaturesmentioning

confidence: 99%

Oceanography and Marine Biology

Hawkins¹,

Evans²,

Dale³

et al. 2018

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Animals living in the Southern Ocean have evolved in a singular environment. It shares many of its attributes with the high Arctic, namely low, stable temperatures, the pervading effect of ice in its many forms and extreme seasonality of light and phytobiont productivity. Antarctica is, however, the most isolated continent on Earth and is the only one that lacks a continental shelf connection with another continent. This isolation, along with the many millions of years that these conditions have existed, has produced a fauna that is both diverse, with around 17,000 marine invertebrate species living there, and has the highest proportions of endemic species of any continent. The reasons for this are discussed. The isolation, history and unusual environmental conditions have resulted in the fauna producing a range and scale of adaptations to low temperature and seasonality that are unique. The best known such adaptations include channichthyid icefish that lack haemoglobin and transport oxygen around their bodies only in solution, or the absence, in some species, of what was only 20 years ago termed the universal heat shock response. Other adaptations include large size in some groups, a tendency to produce larger eggs than species at lower latitudes and very long gametogenic cycles, with egg development (vitellogenesis) taking 18-24 months in some species. The rates at which some cellular and physiological processes are conducted appear adapted to, or at least partially compensated for, low temperature such as microtubule assembly in cells, whereas other processes such as locomotion and metabolic rate are not compensated, and whole-animal growth, embryonic development, and limb regeneration in echinoderms proceed at rates even slower than would be predicted by the normal rules governing the effect of temperature on biological processes. This review describes the current state of knowledge on the biodiversity of the Southern Ocean fauna and on the majority of known ecophysiological adaptations of coldblooded marine species to Antarctic conditions. It further evaluates the impacts these adaptations have on capacities to resist, or respond to change in the environment, where resistance to raised temperatures seems poor, whereas exposure to acidified conditions to end-century levels has comparatively little impact. Zone Description Area (km 2) Length (km) Southern Ocean Total area (km 2)

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The effect of temperature adaptation on the ubiquitin-proteasome pathway in notothenioid fishes

Abstract: There is an accumulating body of evidence suggesting that the subzero Antarctic marine environment places physiological constraints on protein homeostasis. Levels of ubiquitin (Ub)-conjugated proteins,

Cited by 53 publications

References 74 publications

Effects of temperature on juvenile Dungeness crab,Metacarcinus magister(Dana): survival, moulting, and mTOR signalling and neuropeptide gene expression in eyestalk ganglia, moulting gland (Y-organ), and heart

Effects of temperature on juvenile Dungeness crab,Metacarcinus magister(Dana): survival, moulting, and mTOR signalling and neuropeptide gene expression in eyestalk ganglia, moulting gland (Y-organ), and heart

Hepatic transcriptome of the freeze-tolerant Cope’s gray treefrog, Dryophytes chrysoscelis: responses to cold acclimation and freezing

Oceanography and Marine Biology

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