Forty two peach-nectarine (Prunus persica [L.] Batsch.) varieties (i.e Spring Time, Precocissima Morettini, Southhaven, Halehaven, Blazing Gold, Cardinal, Gialla Precoce Morettini, Dixired, Fertilia Morettini, Golden Jubilee, Coronet, Red Haven, Red Globe, Cotonia Massima, Morettini 5/14, Peach of Station, Alexander, Early Elberta, Elberta of Middle Season, Beauty of Georgia, J.H. Hale, Late Elberta, Gold Drest, Morettini No. 1, Jerseyland, Desert Gold, Red Cup, Spring Crest, Loring, June Gold, Merril Gem Free, Early Red, Red Skin, Merril Fortyminer, Roubidoux, Fantasia, Sunhaven, Flavour Top, Merril Franciscan, Blake, Fairtime, Fayette) were studied for 12–18 years in three experimental orchards of Peripheral Agricultural Research Station of Rhodes (PARSR). Their degree of adaptation to the soil and climatic conditions of PARSR was evaluated in terms of yield per tree, time of bloom, time of fruit ripening, fruit quality etc. These results have been compared with related experimental data recorded at the Pomology Institute (PI) at Naoussa. The results indicate that: many peach-nectarine varieties with quite high chilling requirements (Loring, Blake) gave satisfactory yield in PARSR, where the mean average of temperatures below 7°C over one decade was found to be 147 hours (range 52–226). Some peach-nectarine varieties in experimental orchards of PARSR ripened more than ten days earlier (Desert Gold, Early Red, Peach of Station, Loring, Merril Gem Free), of others at about the same time (Coronet, Cotonia Massima, Red Skin, Fertilia Morettini, Red Globe) and at others on a later date (Merril Fortyminer, Early Elberta, J.H. Hale, Cardinal, Southhaven) than they would ripen in northern Greece (PI). Similar differences were also found for the time of blooming.