The effect of temperature on cell growth and astaxanthin accumulation of Haematococcus pluvialis during a light–dark cyclic cultivation

Wan, Minxi; Zhang, Jingkui; Hou, Dongmei; Fan, Jianhua; Li, Yuanguang; Huang, Jianke; Jun, Wang

doi:10.1016/j.biortech.2014.06.030

Cited by 103 publications

(52 citation statements)

References 31 publications

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“…A gas sparger was centrally placed at the bottom. Throughout the experiment, the light intensity was about 540 µmol/ (m 2 s) (Wan et al 2014b) and the culture temperature was controlled at 28 °C. All experiments have two biological replicates.…”

Section: Photoinduction Culture Conditionsmentioning

confidence: 99%

“…Previous studies mainly focused on the optimization of the second stage to improve astaxanthin accumulation ability. Temperature (Wan et al 2014b), strong light (Lv et al 2016), high salinity (Sarada et al 2002), plant hormone (Gao et al 2012a), nitrogen deprivation (Wang et al 2013), oxygen stress (Gu et al 2013), metal ion stress and ethanol (Wen et al 2015) were reported in the second stage to promote H. pluvialis accumulate astaxanthin. Li et al (2019a) and Choi et al (2011) have shown that the appropriate cell type for photoinduction was the non-motile cell, however, there was no article has interpreted this mechanism.…”

Section: Introductionmentioning

confidence: 99%

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Astaxanthin accumulation difference between non-motile cells and akinetes of Haematococcus pluvialis was affected by pyruvate metabolism

Fang

Zhang

Fei

et al. 2020

Bioresour. Bioprocess.

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Background: Haematococcus pluvialis is the best source of natural astaxanthin, known as the king of antioxidants. H. pluvialis have four cell forms: spore, motile cell, non-motile cell and akinete. Spores and motile cells are susceptible to photoinhibition and would die under photoinduction conditions. Photoinduction using non-motile cells as seeds could result in a higher astaxanthin production than that using akinetes. However, the mechanism of this phenomenon has not been clarified. Results:Transcriptome was sequenced and annotated to illustrate the mechanism of this phenomenon. All differentially expressed genes involved in astaxanthin biosynthesis were up-regulated. Particularly, chyb gene was up-regulated by 16-fold, improving the conversion of β-carotene into astaxanthin. Pyruvate was the precursor of carotenoids biosynthesis. Pyruvate kinase gene expression level was increased by 2.0-fold at the early stage of akinetes formation. More changes of gene transcription occurred at the early stage of akinetes formation, 52.7% and 51.9% of total DEGs in control group and treatment group, respectively. Conclusions:Genes transcription network was constructed and the synthesis mechanism of astaxanthin was clarified. The results are expected to further guide the in-depth optimization of the astaxanthin production process in H. pluvialis by improving pyruvate metabolism.

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Section: Photoinduction Culture Conditionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Astaxanthin accumulation difference between non-motile cells and akinetes of Haematococcus pluvialis was affected by pyruvate metabolism

Fang

Zhang

Fei

et al. 2020

Bioresour. Bioprocess.

Self Cite

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“…To increase biomass and astaxanthin, culture parameters such as medium composition, light source, pH, temperature and predators should also be considered. Research have been conducted on a two-stage "perfusion culture" system which can be considered most efficient technology for high productivity, lower water consumption and less contamination (Park et al, 2014;Wan et al, 2014;. Whereas pH and light source can basically improve the yield of biomass and astaxanthin.…”

Section: Sm Jung Et Al: Enhancing Astaxanthin Production From Haemmentioning

confidence: 99%

Enhancing production-extraction and antioxidant activity of astaxanthin from Haematococcus pluvialis

Jung¹,

Jeon²,

Park³

et al. 2019

JEB

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Aim:Methodology: Results:Interpretation: Key words:The growth of H. pluvialis by various extraction methods and was studied its growth, photosynthetic pigments and antioxidative ability under visible light and varying pH was compared.This study compared the growth of H. pluvialis under four different LED lights and three pH values, and demonstrated the astaxanthin content and antioxidant effects of them. The supercritical fluid chromatography and solvent extraction were conducted to compare the astaxanthin contents.The highest growth rate was observed under green LED light at all pH condition (6, 8, 10). Under 2 green LED, the correlation (R ) between cell counts and chlorophyll a, b and total carotenoid was 0.9581, 0.7652 and 0.9095, respectively. The solvent extraction of astaxanthin from H. pluvialis was 137% higher than that of solid extract injection method using Super Fluid Chromatography.This study will contribute in establishing the culture conditions to enhance the biomass and astaxanthin from H. pluvialis.

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“…Although synthetic astaxanthin dominates the world market, currently microalgae cultures are extensively used for the industrial production of biomass as astaxanthin source [5][6][7] . The unicellular green alga Haematococcus pluvialis is a commercial natural source of astaxanthin and it is produced in large industrial scale [8][9][10][11] . In specific stressful conditions such as depletion of nutrients or exposure to high light, H. pluvialis undergoes a morphological transition from green active cells to red cyst cells in which astaxanthin accumulates in the cyst reaching approximately the 5% of cell dry weight 12,13,14 .…”

Section: Introductionmentioning

confidence: 99%

Application of an optimized electrochemical sensor for monitoring astaxanthin antioxidant properties against lipoperoxidation

et al. 2015

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The effect of temperature on cell growth and astaxanthin accumulation of Haematococcus pluvialis during a light–dark cyclic cultivation

Cited by 103 publications

References 31 publications

Astaxanthin accumulation difference between non-motile cells and akinetes of Haematococcus pluvialis was affected by pyruvate metabolism

Astaxanthin accumulation difference between non-motile cells and akinetes of Haematococcus pluvialis was affected by pyruvate metabolism

Enhancing production-extraction and antioxidant activity of astaxanthin from Haematococcus pluvialis

Application of an optimized electrochemical sensor for monitoring astaxanthin antioxidant properties against lipoperoxidation

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