The Effect of Temperature on the Structure and Function of a Cellulose-Degrading Microbial Community

Lu, Yitong; Li, Ning; Gong, De; Wang, Xiaofen; Cui, Zongjun

doi:10.1007/s12010-012-9731-7

Cited by 19 publications

(7 citation statements)

References 34 publications

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“…Furthermore, the proportion increased in the digester subjected to a temperature rise, from 36% at 37°C (R 37 SS) to 64.8% at 44°C (R 44 SS) and 98.0% at 52°C (R 52 SS). An increase in the abundance of Clostridia in response to temperature has been reported in previous studies on biogas digesters degrading a variety of different substrates [19,35,36].…”

Section: Clone Libraries and Phylogenetic Analysissupporting

confidence: 64%

“…Numerous studies have examined anaerobic cellulosedegrading bacteria and their enzymatic capabilities in order to clarify the degradation mechanisms and identify ways to enhance degradation rates. However, most of these studies have been performed on samples from gut and soil ecosystems [3,13,15,16] and only a few have examined cellulose-degrading bacteria in biogas digesters [17][18][19].…”

Section: Introductionmentioning

confidence: 99%

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Biogas production from wheat straw: community structure of cellulose-degrading bacteria

2013

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Background: Wheat straw is one of the most abundant crop residues produced in the world, making it highly interesting as a substrate for biogas production. However, due to the complex structure, its degradability and gas yield are low. The degradability can be improved by pre-treatment, making the material more accessible to microbial degradation. Methods: To investigate the microbial response to straw as a feed stock for biogas production, this study examined the community structure of cellulose-degrading bacteria in lab-scale biogas digesters operating with manure, alone or in co-digestion with straw, with and without pre-treatment (steam-explosion) at different temperatures. The community was studied by targeting the functional gene encoding glycoside hydrolases of families 5 and 48 using T-RFLP, clone libraries and qPCR. Results: In general, bacteria belonging to the phyla Firmicutes and Bacteroidetes dominated the cellulose-degrading bacteria community in all digesters. The degree of similarity to the characterised bacteria was often low, and some clones were more closely related to the uncultured bacteria. The addition of straw, pre-treatment of straw and increasing operating temperature all affected the cellulose-degrading community structure, with differing responses in the cel48 and cel5 communities. Both communities changed in response to temperature, while only the cel5 community was affected by the addition of straw and cel48 community by straw pre-treatment. Conclusions: The addition of straw, pre-treatment of straw and operating temperature all affected the cellulosedegrading community in biogas digesters, but there were no major differences in the digester performance and gas yield.

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Section: Clone Libraries and Phylogenetic Analysissupporting

confidence: 64%

Section: Introductionmentioning

confidence: 99%

Biogas production from wheat straw: community structure of cellulose-degrading bacteria

2013

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“…A number of studies have analyzed community succession during enrichment on plant polysaccharides ( 18 , 20 , 21 , 43 , 44 ). Here, we employed an experimental design allowing us to monitor cellulolytic ability (by observing time to break the filter paper) and community succession (through sequencing) in parallel.…”

Section: Discussionmentioning

confidence: 99%

Long-Term Cellulose Enrichment Selects for Highly Cellulolytic Consortia and Competition for Public Goods

et al. 2022

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“…Since the species of the animal (Li et al, 2012), its rearing environment, and temperature (Lu et al, 2012) greatly influence the intestinal microbiota, their structure and characteristics are not thoroughly reflected through the traditional culture methods. Currently, the largest volume of sequence data exists for intestinal microbial communities, facilitating direct comparisons across multiple studies, which is hindered by some data types such as T-RFLP or DGGE (Oakley et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Age-Related Variations in Intestinal Microflora of Free-Range and Caged Hens

et al. 2017

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Free range feeding pattern puts the chicken in a mixture of growth materials and enteric bacteria excreted by nature, while it is typically unique condition materials and enteric bacteria in commercial caged hens production. Thus, the gastrointestinal microflora in two feeding patterns could be various. However, it remains poorly understood how feeding patterns affect development and composition of layer hens’ intestinal microflora. In this study, the effect of feeding patterns on the bacteria community in layer hens’ gut was investigated using free range and caged feeding form. Samples of whole small intestines and cecal digesta were collected from young hens (8-weeks) and mature laying hens (30-weeks). Based on analysis using polymerase chain reaction-denaturing gradient gel electrophoresis and sequencing of bacterial 16S rDNA gene amplicons, the microflora of all intestinal contents were affected by both feeding patterns and age of hens. Firmicutes, Bacteroidetes, Actinobacteria, Proteobacteria, and Fusobacteria were the main components. Additionally, uncultured environmental samples were found too. There were large differences between young hens and adult laying hens, the latter had more Firmicutes and Bacteroidetes, and bacterial community is more abundant in 30-weeks laying hens of all six phyla than 8-weeks young hens of only two phyla. In addition, the differences were also observed between free range and caged hens. Free range hens had richer Actinobacteria, Bacteroidetes, and Proteobacteria. Most of strains found were detected more abundant in small intestines than in cecum. Also the selected Lactic acid bacteria from hens gut were applied in feed and they had beneficial effects on growth performance and jejunal villus growth of young broilers. This study suggested that feeding patterns have an importance effect on the microflora composition of hens, which may impact the host nutritional status and intestinal health.

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The Effect of Temperature on the Structure and Function of a Cellulose-Degrading Microbial Community

Cited by 19 publications

References 34 publications

Biogas production from wheat straw: community structure of cellulose-degrading bacteria

Biogas production from wheat straw: community structure of cellulose-degrading bacteria

Long-Term Cellulose Enrichment Selects for Highly Cellulolytic Consortia and Competition for Public Goods

Age-Related Variations in Intestinal Microflora of Free-Range and Caged Hens

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