The effect of temperature on the potential difference and input resistance of rat seminiferous tubules

Gladwell, R. T.

doi:10.1113/jphysiol.1977.sp011849

Cited by 8 publications

(6 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…, 2004). This report is consistent with the early evidence that elevation of testicular temperature affected morphology and function of SCs (Hagenas & Ritzen, 1975; Gladwell, 1977). One of the main functions of the BTB is regulating the movement of products both into and out of the seminiferous epithelium (Meng et al.…”

Section: Discussionsupporting

confidence: 92%

Scrotal heat stress causes a transient alteration in tight junctions and induction of TGF-β expression

Cai¹,

Ren²,

et al. 2010

International Journal of Andrology

View full text Add to dashboard Cite

Specialized junctions, which occur at sites of Sertoli-Sertoli and Sertoli-germ cell contact of seminiferous epithelium, play pivotal roles in spermatogenesis. Slight increase in scrotal temperature can induce oligospermia or azoospermia via increasing germ cell apoptosis. In this study, we demonstrated that the expression of tight junction (TJ) components, such as occludin, claudin-3 and zonula occludens-1 (ZO-1), was reduced 24-48h after a single mild scrotal heat exposure (43°C for 30min), whereas mRNA levels of claudin-11 were increased. Moreover, the protein localization of occludin and ZO-1 was lost from the blood-testis barrier (BTB) site, whereas claudin-11 immunostaining became diffuse and cytoplasmic 2days following heat exposure. Electron microscopic analysis showed that 2days after the heat treatment, the intercellular space between the two adjacent Sertoli cells was expanded, coupled with defragmentation of actin bundles and the endoplasmic reticulum. In addition, the TJ permeability increased significantly 2days after the heat exposure and recovered approximately 10days later. Heat-induced reversible BTB disruption was associated with a transient induction of transforming growth factor (TGF)-β2, -3 and p38 mitogen-activated protein kinase activation. However, the TGF-β antagonist only partially prevented the heat-induced BTB disruption. In conclusion, the expression of TJ-associated molecules and BTB were reversibly perturbed after mild testicular hyperthermia, and the induction of TGF-β expression may be partially involved in heat-induced BTB damage.

show abstract

Section: Discussionsupporting

confidence: 92%

Scrotal heat stress causes a transient alteration in tight junctions and induction of TGF-β expression

Cai¹,

Ren²,

et al. 2010

International Journal of Andrology

View full text Add to dashboard Cite

show abstract

“…Given the marked fall in testicular venous oxygen content, therefore, oxygen supply to the germinal epithelium of the rat may become limiting at high temperatures, as also suggested for the ram (Waites & Setchell, 1964). Thus the heat-induced stimulation followed by a decline in potas¬ sium exchange and the same pattern of change in transtubular potential (Gladwell, 1977) could both be due to a progressive inhibition of the active transport of potassium because of oxygen deficiency. This would explain reports that the uptake of 86Rb by the whole testis (Setchell et al, 1969) and also by isolated seminiferous tubules in vitro (Setchell & Singleton, 1971) was reduced at temperatures higher than 33°C.…”

Section: Discussionmentioning

confidence: 99%

“…The increase in rates of exchange of 86Rb, 42K,lysine and some 3H-labelled steroids with tempera¬ ture is not unexpected. However, the early maximal stimulation of 86Rb and 42K movements followed by a decline in apparent exchange rate is less readily explicable and merits further investigation, especially as a pattern of initial hyperpolarization followed by depolarization of the seminiferous tubules has been observed at the same temperature (Gladwell, 1977).…”

Section: Discussionmentioning

confidence: 99%

The blood-testis barrier and temperature damage to the testis of the rat

Main¹,

Waites²

1977

Reproduction

View full text Add to dashboard Cite

Summary. The integrity of the blood\p=n-\testisbarrier was investigated during and after local heating of rat testes sufficient to produce a temporary cessation of spermatogenesis. The flow, ionic composition and protein content of rete testis fluid (RTF) collected from testes maintained at 33 or 41\s=deg\Cwere unaffected either at the time of treatment or up to 2 days later when the major cytological consequences of heating occurred. The normally low rate of transfer of albumin from blood to RTF was unaffected during and after heating. Transfer constants for radioactive K, Rb, Na and lysine consistently increased during heating although there were time-dependent differences between the patterns of response for each molecule. The normally rapid transfer of testosterone was unaffected by heating, but the entry rates of radioactivity into RTF after the infusion of more slowly diffusing steroids were enhanced at 41\s=deg\C. The clearest effects of heating were an approximate doubling in the uptake of oxygen and decrease in the net synthesis of protein by the testis. It is concluded that heating sufficient to damage spermatogenesis was not associated with dramatic alterations in the integrity of the blood\p=n-\ testis barrier but more with changes in testicular metabolism.

show abstract

“…[15] FSH and LH levels have been reported to be elevated in azoospermia (Dada R et al ., 2010). [5] This suggests a negative functional relationship between secretion of FSH and this step of germ cell maturation.…”

Section: Discussionmentioning

confidence: 99%

“…[4] Primary lesion of heat inactivation of the testes is hypoxia in the germinal epithelium. [5] Dada et al . (2010),[6] and many other researchers have substantially reported spermatogenic arrest due to heat stress leading to low sperm counts.…”

Section: Introductionmentioning

confidence: 99%

Abhraka Bhasma treatment ameliorates proliferation of germinal epithelium after heat exposure in rats

Bhatia¹,

Kale

Daoo³

et al. 2012

Ancient Sci Life

View full text Add to dashboard Cite

This study was conducted to evaluate the protective effect of Abhraka Bhasma on spermatogenesis in heat-damaged testis. A histological analysis over the sukravaha srotomula (testes) of male albino Wistar rat was carried out in order to examine the potency of the test drug in preventing the organ from heat damage. The current experiment was carried out on 32 healthy adult male albino Wistar rats divided into four groups. Sahastraputi Abhraka Bhasma, subjected to 1000 putas, was used as the test drug. On sacrificing the animals after 30 days, it was observed that control animals (G1) had normal spermatogenesis and drug-induced animals (G2) showed hyperactive tubules. Testicular hyperthermia occurred in few (G3) animals, who were subjected to 43°C for 1 h daily for four consecutive weeks, resulting in degeneration of tubules with inspissated spermatozoa (25%) leading to atrophy of the organ. 3% tubules showed disintegration, 23% were in the recovery stage while 71% tubules exhibited enhanced proliferation of germinal epithelium leading to hypertrophy and hyperplasia. The present study reveals that the test drug can correct heat-induced male infertility and provides us with the possibility of treatment of human heat-induced oligozoospermia and azoospermia. Hence, this ayurvedic maharasa (primary mineral) can be a promising formulation as an anti-impotency fecundity drug.

show abstract

The effect of temperature on the potential difference and input resistance of rat seminiferous tubules

Cited by 8 publications

References 20 publications

Scrotal heat stress causes a transient alteration in tight junctions and induction of TGF-β expression

Scrotal heat stress causes a transient alteration in tight junctions and induction of TGF-β expression

The blood-testis barrier and temperature damage to the testis of the rat

Abhraka Bhasma treatment ameliorates proliferation of germinal epithelium after heat exposure in rats

Contact Info

Product

Resources

About