The Effect of Thawing Temperature on Sperm Quality of Friesian Holstein Bulls

Utami, Tri; Topianong, Tarsisius Considus

doi:10.22146/jsv.5420

Cited by 3 publications

(3 citation statements)

References 6 publications

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“…Yendraliza et al (2019) showed abnormalities of frozen semen of Bali cattle in Andromed ® diluent, with various percentages of carrot juice after thawing, to be around 5.17% to 10.50%. Utami and Tophianong (2014) showed that the highest abnormality of sperm thawed in water at 8 o C and 37 o C was 20% and 23%. Abnormal data in previous studies using frozen semen of non-sexed Limousine cattle was 6.93 ± 1.37% (Mahfud et al 2019).…”

Section: Intact Plasma Membrane (Ipm) Simmental Frozenmentioning

confidence: 99%

Frozen Semen Quality of Simmental Cattle in Various Commercial Diluents

Komariah

Zuhdi

Tahar

et al. 2023

JIPTHP

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This study determines the effect of different commercial diluents on the quality of frozen Simmental semen. The extraction of fresh semen from 3 Simmental bulls using an artificial vaginal method was performed twice a week. Fresh semen with motility of >70% was further processed to frozen using a one-step dilution method, and the commercial diluents used were Andromed®, Optixcell®, and Steridyl®. The diluted semen was packed into mini-straws, equilibrated, frozen, and stored for further testing. Data were analyzed by analysis of variance (ANOVA), followed by Duncan Multiple Range Test (DMRT) analysis with a 5% confidence level. The results showed that commercial diluents significantly affected motility, viability, intact plasma membrane, abnormalities, and recovery rate of frozen semen (P<0.05). Commercial diluents with different sources of lecithin reported different results on the quality of frozen semen. Steridyl® has a more optimal ability to maintain the quality of frozen semen of Simmental cattle, followed by Optixcell® and Andromed®.

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Section: Intact Plasma Membrane (Ipm) Simmental Frozenmentioning

confidence: 99%

Frozen Semen Quality of Simmental Cattle in Various Commercial Diluents

Komariah

Zuhdi

Tahar

et al. 2023

JIPTHP

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“…Normal spermatozoa motility ranges from 70-90% with fast movement [29]. Spermatozoa move because of the role of the spermatozoa tail, due to the tail of the spermatozoa containing many mitochondria that act as energy producers in the form of ATP so that spermatozoa move forward [30]. Sperm motility of ejaculate had been affected by the temperature at the time of storage and during examination.…”

Section: Issn 2087-2852 E-issn 2338-1655mentioning

confidence: 99%

The Effect of Alpinia Purpurata K. Schum Rhizome Extract Supplementation in Tris-Egg Yolk Extender on the Quality of Cryopreserved Bali Bull Sperm

Putri,

Rahayu,

Marhendra

2023

JELS

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This study aimed to analyze the effect of Alpinia purpurata K. Schum rhizome extract (APRE) supplementation into tris-egg yolk extender on the sperm quality of Bali bull after freezing. This study used experimental method with a completely randomized design (CRD), consisting of five treatments, denoted as P0 (semen diluted using 100% Tris-Egg Yolk (TEY)), P1 (semen diluted using 98% TEY and 2% APRE), P2 (semen diluted using 96% TEY extender + 4% APRE), P3 (semen diluted using 94% TEY + 6% APRE), and P4 (semen diluted using 92% TEY + 8% APRE). Semen quality was assessed both before and after freezing, using several indicators such as motility, viability, and abnormality of sperm. The antioxidant activity of RGE was determined using the DPPH (1,1-diphenils-2picrylhydrazils) technique. The data were analyzed using ANOVA with further analysis using post hoc tests in the SPSS 25 program. The results showed that APRE has strong antioxidant activity, with an IC50 value 38.004 μg.mL -1 . Meanwhile, this research has shown that the addition of 6% RGE in the tris-Tris-egg yolk extender was able to maintain sperm quality before and after freezing.

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“…It can be concluded that this semen deserves to be processed further. Likewise, Utami et al (2014) stated that spermatozoa abnormalities were no more than 12% to 23% and the normal percentage was between 77% to 88% in livestock. However, the result of this study was lower than that of Rahmiati et al (2018) with an average abnormality of 10.34% in Aceh cattle and higher than that of Kaiin and Kaiin and Gunawan (2015) with abnormalities of 1.47% in Simmental bull previously reared at RAIC Pucak.…”

Section: Advances In Animal and Veterinary Sciencesmentioning

confidence: 99%

Bali Bull, Fresh Semen, Frozen Semen, Semen Quality, Simmental Bull

Adiputra¹,

Maulana²,

Kaiin³

et al. 2022

AAVS

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This study was conducted to determine the quality fresh and frozen semen of Bali and Simmental bull in the Technical Implementation Unit of Regional Artificial Insemination Center at Pucak, South Sulawesi. Two Bali bulls and two Simmental bulls aged 5-6 were used. The parameters observed in the fresh semen were volume, pH, colour, motility, viability, abnormality, intact plasma membrane, and concentration. Meanwhile, parameters observed in frozen semen were motility, viability, abnormality, intact plasma membrane, acrosome status, DNA integrity, and movement patterns. Viability and abnormality parameters were stained with eosin-nigrosine, intact plasma membrane was stained with Hypo-osmotic (HOS) solution, acrosome status was stained with peanut agglutinin (PNA), and DNA integrity was stained with acridine orange (AO). The results showed that the volume, pH, motility, abnormality, intact plasma membrane, and concentration of fresh semen of Bali and Simmental bull were not significantly different (P>0.05). Likewise, the frozen semen of Bali and Simmental breed did not show any significant differences (P>0.05) in terms of motility, acrosome status, intact plasma membrane, DNA integrity, and movement patterns, but the viability of Bali bull was significantly higher than that of Simmental bull and the abnormality of Simmental bull was significantly higher than that of Bali bull. Based on the results, it can be concluded both Bali and Simmental bull has the same quality of fresh semen, but Bali bull had better viability and abnormality compared to those Simmental breed of frozen semen.

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The Effect of Thawing Temperature on Sperm Quality of Friesian Holstein Bulls

Cited by 3 publications

References 6 publications

Frozen Semen Quality of Simmental Cattle in Various Commercial Diluents

Frozen Semen Quality of Simmental Cattle in Various Commercial Diluents

The Effect of Alpinia Purpurata K. Schum Rhizome Extract Supplementation in Tris-Egg Yolk Extender on the Quality of Cryopreserved Bali Bull Sperm

Bali Bull, Fresh Semen, Frozen Semen, Semen Quality, Simmental Bull

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