The effect of the addition of a polyglutamate motif to RGD on peptide tethering to hydroxyapatite and the promotion of mesenchymal stem cell adhesion

Sawyer, Andrew J.; Weeks, David M.; Kelpke, Stacey S.; McCracken, Michael S.; Bellis, Susan L.

doi:10.1016/j.biomaterials.2005.05.006

Cited by 70 publications

(74 citation statements)

References 48 publications

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“…Moreover, their results suggested that RGD modification was even more efficient at inducing chondrogenic differentiation than when exogenous type I collagen (which contains RGD) was incorporated into the matrix [132]. Several studies indicated that RGDmodified PLLA and hydroxyapatite scaffolds improved hMSCs adherence to the matrix [133,134]. Additional studies indicated that RGD-decoration of PEG hydrogels improved MSC viability [135] and promoted osteogenesis of MSCs in a dosage-dependent manner, as judged by early and late bone marker expression.…”

Section: Introducing Adhesion Peptidesmentioning

confidence: 93%

Microenvironment Design for Stem Cell Fate Determination

Ra'em

Cohen

2011

Tissue Engineering III: Cell - Surface Interactions for Tissue Culture

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Stem cells are characterized by their dual ability for self-renewal and differentiation, potentially yielding large numbers of cells that can be used in cell therapy and tissue engineering for repairing devastating diseases. Attaining control over stem cell fate decision in culture is a great challenge since these cells integrate a complex array of “niche” signals, which regulate their fate. Given this, the recent findings that synthetic microenvironments can be designed to gain some level of control over stem cell fate are encouraging. This chapter provides an overview of the current state and knowledge of the design of synthetic microenvironments bio-inspired by the adult stem cell niche. We describe the biomaterials used for reconstituting the niche, highlighting the bioengineering principles used in the process. Such synthetic microenvironments constitute powerful tools for elucidating stem cell regulatory mechanisms that should fuel the development of advanced culture systems with accurate regulation of stem cell fate.

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Section: Introducing Adhesion Peptidesmentioning

confidence: 93%

Microenvironment Design for Stem Cell Fate Determination

Ra'em

Cohen

2011

Tissue Engineering III: Cell - Surface Interactions for Tissue Culture

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“…Stable immobilization of peptides on the surface of biomaterials is essential to promote strong cell adhesion. RGD peptides commonly covalently bind to the surface of biomaterials via functional moieties such as hydroxyl, amino, and carboxyl radicals (Sawyer et al 2005). However, many problems may Dan Wang and Jing Mao have contributed to this paper equally.…”

Section: Introductionmentioning

confidence: 99%

A chimeric peptide that binds to titanium and mediates MC3T3-E1 cell adhesion

et al. 2010

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Our study provides a promising alternative of biomimetic coating which functionalizes the dental implant with adhesion peptides and may be useful for enhancing the bone remodeling around Ti implants. A chimeric peptide consisting of an Arg-Gly-Asp (RGD) sequence (mediating cell adhesion) and a RKLPDA (minTBP-1) sequence (specifically recognizing and binding to Ti substrate) was designed and synthesized. The chimeric peptide affinity to Ti disks, as well as its role in mediating MC3T3-E1 cell attachment and afterwards spreading on pre-coated Ti disks, was investigated. The chimeric peptide not only showed favorable affinity to Ti surfaces but also facilitated the adhesion of MC3T3-E1 cells.

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“…An even more downscaled approach is to attach an RGD sequence to a septa-glutamic acid. 196 The glutamic acid residue bound to the apatite, and the RGD sequence attracted mesenchymal stem cells more effectively than the RGD without the septa-Glu. A recent study has cast a doubt on this type of approach because it was claimed that since apatite is so prone to protein adsorption, any preattached cell ligands will be covered by an array of blood proteins, 197 although the approach still could be useful to improve cell seeding prior to implantation.…”

Section: Chimeric Peptidesmentioning

confidence: 99%

Proteins and Their Peptide Motifs in Acellular Apatite Mineralization of Scaffolds for Tissue Engineering

Benesch

Mano

Reis

2008

Tissue Engineering Part B: Reviews

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Many proteins in the inorganic=organic matrix of bone induce or modulate or inhibit mineralization of apatite in vivo. Many attempts have been made to mimic and understand this mechanism as part of bone formation, and ectopic mineralization and control thereof. Many attempts have also been made to use such proteins or protein fragments to harness their potential for improved mineralization. Such proteins and peptide motifs have also been the inspiration for attempts of making mimics of their structures and motifs using chemical or biological synthesis. The aim of this review is to highlight how proteins and (poly)peptides themselves impact mineralization in the human body, and how those could be used and have been used for improving apatite mineralization, for example, on or in materials that by themselves do not induce apatite mineralization but otherwise have interesting properties for use as bone tissue engineering scaffolds.

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The effect of the addition of a polyglutamate motif to RGD on peptide tethering to hydroxyapatite and the promotion of mesenchymal stem cell adhesion

Cited by 70 publications

References 48 publications

Microenvironment Design for Stem Cell Fate Determination

Microenvironment Design for Stem Cell Fate Determination

A chimeric peptide that binds to titanium and mediates MC3T3-E1 cell adhesion

Proteins and Their Peptide Motifs in Acellular Apatite Mineralization of Scaffolds for Tissue Engineering

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