The Effect of the Aerial Part of Lindera akoensis on Lipopolysaccharides (LPS)-Induced Nitric Oxide Production in RAW264.7 Cells

Abstract

Four new secondary metabolites, 3α-((E)-Dodec-1-enyl)-4β-hydroxy-5β-methyldihydrofuran-2-one (1), linderinol (6), 4′-O-methylkaempferol 3-O-α-l-(4″-E-p-coumaroyl)rhamnoside (11) and kaempferol 3-O-α-l-(4″-Z-p-coumaroyl) rhamnoside (12) with eleven known compounds—3-epilistenolide D1 (2), 3-epilistenolide D2 (3), (3Z,4α,5β)-3-(dodec-11-ynylidene)-4-hydroxy-5-methylbutanolide (4), (3E,4β,5β)-3-(dodec-11-ynylidene)-4-hydroxy-5-methylbutanolide (5), matairesinol (7), syringaresinol (8), (+)-pinoresinol (9), salicifoliol (10), 4″-p-coumaroylafzelin (13), catechin (14) and epicatechin (15)—were first isolated from the aerial part of Lindera akoensis. Their structures were determined by detailed analysis of 1D- and 2D-NMR spectroscopic data. All of the compounds isolated from Lindera akoensis showed that in vitro anti-inflammatory activity decreases the LPS-stimulated production of nitric oxide (NO) in RAW 264.7 cell, with IC50 values of 4.1–413.8 μM.

Quali-quantitative analysis of the phenolic fraction of the flowers of Corylus avellana, source of the Italian PGI product “Nocciola di Giffoni”: Isolation of antioxidant diarylheptanoids

“…Moreover, compounds 1e9 were identified by analysis of spectroscopic data in comparison to those reported in literature as (Matlawska et al, 1994) (Kirmizibekmez et al, 2014), quercetin 3-O-a-L-rhamnopyranoside (4) (Materska et al, 2003), giffonin I (5) (Masullo et al, 2015b), kaempferol 3-O-a-Lrhamnopyranoside (6) (Ahmed et al, 1989), kaempferol 3-O-(4 00 -cis-p-coumaroyl)-a-L-rhamnopyranoside (7) (Yang et al, 2013), kaempferol 3-O-(4 00 -trans-p-coumaroyl)-a-L-rhamnopyranoside (8) (Yang et al, 2013), and alnusone (9) (Masullo et al, 2015b).…”

Three Pathways Assess Anti-Inflammatory Response of Epicatechin with Lipopolysaccharide-Mediated Macrophage RAW264.7 Cells

“…The work of Yang et al . () indicated that epicatechin isolated from the aerial part of Lindera akoensis could decrease the LPS‐stimulated NO production in RAW264.7 cell without exploring the related mechanisms.…”

Anti-Inflammatory and Analgesic Effects of the Marine-Derived Compound Excavatolide B Isolated from the Culture-Type Formosan Gorgonian Briareum excavatum

“…Inflammation plays an important role in the development of many diseases, and different cell types are recruited, including monocytes/macrophages, neutrophils and lymphocytes, to the tissue during the process of inflammation [ 12 , 13 , 14 ]. The LPS-stimulated murine macrophage model is a well-known system to assess the anti-inflammatory activities of marine- and terrestrial-derived secondary metabolites in vitro [ 15 , 16 , 17 , 18 ]. LPS could upregulate the production of various pro-inflammatory mediators, including cytokines, chemokines and enzymes, such as COX-2 and iNOS, which play critical roles in inflammatory processes via activation of the nuclear factor (NF)-κB signaling pathway [ 19 , 20 ].…”