The effect of the antioxidant drug U-74389G on urea levels during ischemia reperfusion injury in rats

Abstract

This experimental study examined the effect of the antioxidant drug U-74389G, on a rat model and particularly in a renal ischemia-reperfusion protocol. The effects of that molecule were studied biochemically using blood mean urea levels. Forty rats of mean weight 231.875 g were used in the study. Urea levels were measured at 60 min of reperfusion (groups A and C) and at 120 min of reperfusion (groups B and D). The drug U-74389G was administered only in groups C and D. U-74389G administration significantly decreased the predicted urea levels by 11.35%±2.73% (P=0.0001). Reperfusion time non-significantly increased the predicted urea levels by 2.26%±3.29% (P=0.4103). However, U-74389G administration and reperfusion time together significantly decreased the predicted urea levels by 6.31%±1.70% (P=0.0005). U-74389G administration whether it interacted or not with reperfusion time had significant decreasing effect on the urea serum levels, reflecting a respective renal function augmentation.

The Effect of the Antioxidant Drug “U-74389G” on Creatine Phosphokinase Levels during Ischemia Reperfusion Injury in Rats

“…These membrane-associated antioxidants are particularly effective in preventing permeability changes in brain microvascular endothelial cell monolayers (2). The same authors of that study found the influence of U-74389G, as depicted in Table 1, on some seric variable levels in related IR injury experiments at the same endpoints with the present experiment after clamp removal in rats (3,4). 3693/12-11-2010 and 14/10-1-2012 decisions.…”

“…The experiment started with animals subjected to pre-narcosis followed by general anesthesia, as described in detail in related references (3,4). Their electrocardiogram, acidometry, and oxygen supply were continuously provided.…”

The alterations effects in phosphorus of erythropoietin and U-74389G

“…The unique available study investigating the hypophosphoremic effect of U-74389G was the preliminary one 1 . Although the most famous activities of neuroprotection and membrane-stabilization properties, it accumulates in the cell membrane, protecting vascular endothelium from peroxidative damage but hardly penetrates the blood-brain barrier.…”

“…The Vet licenses under 3693/12-11-2010 & 14/10-1-2012 numbers, the granting company and the experiment location are mentioned in preliminary references [1,2]. The human animal care of Albino female Wistar rats, the 7 days pre-experimental ad libitum diet, the non-stop intraexperimental anesthesiologic techniques, the acidometry, the electrocardiogram, the oxygen supply and post-experimental euthanasia are also described in preliminary references.…”

The Effect of the Antioxidant Drug “U-74389G” on Lactate Dehydrogenase Levels during Ischemia Reperfusion Injury in Rats

“…7 days pre-experimental normal housing included ad libitum diet in laboratory. Prenarcosis of animals proceeded of continuous intra-experimental general anesthesia [3][4][5][6], oxygen supply, electrocardiogram and acidometry. Post-experimental euthanasia did not permitted awakening and preservation of the animals.…”