The effect of the coumarin-like derivative osthole on the osteogenic properties of human periodontal ligament and jaw bone marrow mesenchymal stem cell sheets

Gao, Lina; An, Yu; Lei, Ming; Li, Bei; Yang, Hao; Lü, Hong; Chen, Fa‐Ming; Jin, Yan

doi:10.1016/j.biomaterials.2013.09.017

Cited by 81 publications

(89 citation statements)

References 59 publications

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“…Most importantly, cell sheets make delivering and securing PDLSCs onto the tooth root surface possible, which is a key to PDL tissue regeneration (Dan et al 2014). Therefore, the identification of an appropriate technique for enhancing sheet production from PDLSCs has attracted growing interest (Wei et al 2012;Gao et al 2013). In contrast, the ability of cells to generate robust cell sheets has become a key parameter for evaluating the properties of a target cell population Wu et al 2015).…”

Section: Discussionmentioning

confidence: 98%

“…In particular, many preclinical and clinical studies have suggested that PDLSCs represent an effective therapeutic tool for periodontal regeneration (Bright et al 2015;Bassir et al 2016). In parallel, methods for PDLSC isolation and production are developing very quickly, from rapid and safe cell propagation/expansion (Iwata et al 2010;Jung et al 2013;Trubiani et al 2015;Wu et al 2016) to optimal cell material production (Wei et al 2012;Gao et al 2013;Iwata et al 2015). Nevertheless, major challenges still exist, particularly challenges related to producing large numbers of cells from clinically limited PDL tissues and manufacturing/banking robust cell materials, ideally in an off-the-shelf format Tang et al 2016;Wu et al 2016).…”

Section: Discussionmentioning

confidence: 99%

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Effects of short-term inflammatory and/or hypoxic pretreatments on periodontal ligament stem cells: in vitro and in vivo studies

et al. 2016

Cell Tissue Res

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In this study, we extensively screened the in vitro and in vivo effects of PDLSCs following short-term inflammatory and/or hypoxic pretreatments. We found that the 24-h hypoxic pretreatment of PDLSCs significantly enhanced cell migration and improved cell surface CXCR4 expression. In addition, hypoxia-pretreated PDLSCs exhibited improved cell colony formation and proliferation. Cells that were dually stimulated also formed more colonies compared to untreated cells but their proliferation did not increase. Importantly, the hypoxic pretreatment of PDLSCs enhanced cell differentiation as determined by elevated RUNX-2 and ALP protein expression. In this context, the inflammatory stimulus impaired cell OCN protein expression, while dual stimuli led to decreased RUNX-2 and OCN mRNA levels. Although preconditioning PDLSCs with inflammatory and/or hypoxic pretreatments resulted in no differences in the production of matrix proteins, hypoxic pretreatment led to the generation of thicker cell sheets; the inflammatory stimulus weakened the ability of cells to form sheets. All the resultant cell sheets exhibited clear bone regeneration following ectopic transplantation as well as in periodontal defect models; the amount of new bone formed by hypoxia-preconditioned cells was significantly greater than that formed by inflammatory stimulus- or dual-stimuli-treated cells or by nonpreconditioned cells. The regeneration of new cementum and periodontal ligaments was only identified in the hypoxia-stimulus and no-stimulus cell groups. Our findings suggest that PDLSCs that undergo short-term hypoxic pretreatment show improved cellular behavior in vitro and enhanced regenerative potential in vivo. The preconditioning of PDLSCs via combined treatments or an inflammatory stimulus requires further investigation.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Effects of short-term inflammatory and/or hypoxic pretreatments on periodontal ligament stem cells: in vitro and in vivo studies

et al. 2016

Cell Tissue Res

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“…Finally, the dishes were incubated at 37°C in a humidified atmosphere containing 5% CO 2 , and the medium was freshly changed every 3 days until the primary cells migrated from the tissue blocks and reached confluence. As we have reported previously [23], the limiting dilution technique was used to purify stem cells from the primary cells, and the resultant cells at passages 1-3 (P1-P3) were used in subsequent studies to avoid cell behavioral changes due to long-term in vitro culture.…”

Section: Isolation and Purification Of Pdlscsmentioning

confidence: 99%

“…If cell sheet technology is used for cell delivery, the functionality of the cell sheets used in the trials, which may be influenced by the cell populations employed [21] as well as by the methods used for cell sheet production [23][24][25], must be predicted. Previously published data have demonstrated that the multipotent capacity and regenerative potential of human PDLSCs are severely compromised with increasing donor age [26,27].…”

Section: Introductionmentioning

confidence: 99%

Age-related decline in the matrix contents and functional properties of human periodontal ligament stem cell sheets

et al. 2015

Acta Biomaterialia

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“…It had been widely reported that Osthole exerts a significant positive effect on osteoporosis treatment by promoting osteoblast differentiation and inhibiting bone resorption [10][11][12][13][14]. Interestingly, a recent study on biomaterial suggested that Osthole stimulation may enhance extracellular matrix (ECM) production and positively affect cell behavior in cell sheet engineering of jaw bone marrow mesenchymal stem cells [15]. However, further studies investigating the impact of Osthole on the function of bone marrow-derived MSCs and the underlying mechanism remain quite limited.…”

Section: Introductionmentioning

confidence: 98%

An Inhibitory Role of Osthole in Rat MSCs Osteogenic Differentiation and Proliferation via Wnt/β-Catenin and Erk1/2-MAPK Pathways

Chen

Dai

et al. 2016

Cell Physiol Biochem

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Background/Aims: Bone marrow-derived mesenchymal stem cells (MSCs) are responsible for new bone formation during adulthood. Accumulating evidences showed that Osthole promotes the osteogenic differentiation in primary osteoblasts. The aim of this study was to investigate whether Osthole exhibits a potential to stimulate the osteogenic differentiation of MSCs and the underlying mechanism. Methods: MSCs were treated with a gradient concentration of Osthole (6.25 µM, 12.5 µM, and 25 µM). Cell proliferation was assessed by western blotting with the proliferating cell nuclear antigen (PCNA) and Cyclin D1 antibodies, fluorescence activated cell sorting (FACS), and cell counting kit 8 (CCK8). MSCs were cultured in osteogenesis-induced medium for one or two weeks. The osteogenic differentiation of MSCs was estimated by Alkaline Phosphatase (ALP) staining, Alizarin red staining, Calcium influx, and quantitative PCR (qPCR). The underlying mechanism of Osthole-induced osteogenesis was further evaluated by western blotting with antibodies in Wnt/β-catenin, PI3K/Akt, BMPs/smad1/5/8, and MAPK signaling pathways. Results: Osthole inhibited proliferation of rat MSCs in a dose-dependent manner. Osthole suppressed osteogenic differentiation of rat MSCs by down-regulating the activities of Wnt/β-catenin and Erk1/2-MAPK signaling. Conclusions: Osthole inhibits the proliferation and osteogenic differentiation of rat MSCs, which might be mediated through blocking the Wnt/β-catenin and Erk1/2-MAPK signaling pathways.

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The effect of the coumarin-like derivative osthole on the osteogenic properties of human periodontal ligament and jaw bone marrow mesenchymal stem cell sheets

Cited by 81 publications

References 59 publications

Effects of short-term inflammatory and/or hypoxic pretreatments on periodontal ligament stem cells: in vitro and in vivo studies

Effects of short-term inflammatory and/or hypoxic pretreatments on periodontal ligament stem cells: in vitro and in vivo studies

Age-related decline in the matrix contents and functional properties of human periodontal ligament stem cell sheets

An Inhibitory Role of Osthole in Rat MSCs Osteogenic Differentiation and Proliferation via Wnt/β-Catenin and Erk1/2-MAPK Pathways

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