The effect of the growth environment on the serum sensitivity of some urinary Escherichia coli strains

Taylor, Peter W.

doi:10.1016/0378-1097(78)90069-1

Cited by 7 publications

(13 citation statements)

References 7 publications

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“…This indicates that a possible component of cellular resistance may be due to a reversal of the inhibitory effects of the MAC (Dankert 1989). The same strain became sensitive to the bactericidal action of serum when grown in carbon-and magnesium-limited conditions (Taylor 1978;Taylor et al 1981). This sensitivity seemed to be related to a lower lipopolysaccharide O side chain sugar to core-sugar ratio, assessed after one hour of serum incubation (Taylor et al 1981).…”

Section: Introductionmentioning

confidence: 94%

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Induction of complement sensitivity in Escherichia coli by citric acid and low pH

Ocaña-Morgner

Dankert

2001

J Appl Microbiol

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Section: Introductionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Induction of complement sensitivity in Escherichia coli by citric acid and low pH

Ocaña-Morgner

Dankert

2001

J Appl Microbiol

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“…Even so, the reduction in CFU was only in the order of one-half log viability of this organism. Earlier investigators have established that serum susceptibility of bacteria is influenced by the growth conditions employed for preparation of the inoculum (16). E. coli for instance are more readily killed by serum when in stationary phase (3,13).…”

Section: Methodsmentioning

confidence: 99%

Resistance of Pseudomonas pseudomallei to Normal Human Serum Bactericidal Action

Ismail

Razak

Mohamed

et al. 1988

Microbiology and Immunology

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The effect of human normal serum (HNS) on was determined. It is apparent from our data that the organism is resistant to the normal serum bactericidal mechanism. Ancillary experiments to confirm this serum-resistant property of P. pseudomallei were done by examining the effects of growth phase conditions of the bacteria (i.e., logarithmic and stationary phases) and different buffered systems used as diluent in our bactericidal assay. Results obtained showed similar degree of resistance to serum bactericidal killing by 5 strains of the organisms tested. The possible survival advantage of serum-resistant property to P. pseudomallei as bacterial pathogens known to invade the blood stream is discussed.

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“…The response of bacteria grown in nutrient-limited culture to normal human serum is also shown in table I. Serum-sensitivity tests were performed on portions of bacterial suspension removed from the culture vessel during collection of cells for envelope analysis. For serum-sensitivity determination, bacterial samples were immediately diluted in Tris-HC1 pH 8.4 to give a cell concentration of 14-2.0 x 106/ml; 0.5 ml of this ENVIRONMENT AND E. COLI ENVELOPES I I suspension was added to 1.5 ml of human serum and viable counts were obtained by the pour-plate technique at the beginning of each test and after incubation for 1,2 and 3 h at 37°C (Taylor, 1978).…”

Section: Methodsmentioning

confidence: 99%

“…In a recent study (Taylor, 1978) the sensitivity of chemostat-grown strains o f E. coli to normal human serum was investigated. There was a tendency for slow-growing cells to be more resistant than rapidly-growing cells and for magnesium ion-limited (Mg-lim) cultures to be more sensitive than carbon-limited (C-lim) cultures.…”

Section: Introductionmentioning

confidence: 99%

Effect of the growth environment on cell-envelope components of Escherichia coli in relation to sensitivity to human serum

Taylor

Messner

Parton

1981

Journal of Medical Microbiology

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SUMMARY.Three smooth strains of urinary Escherichia coli were grown in a chemostat under carbon-limited (C-lim) and magnesium-limited (Mg-lim) conditions over a range of dilution rates (D). Strain LP1674 was resistant to human serum under C-lim but became sensitive when grown under Mg-lim, the degree of sensitivity increasing as D increased. The transition to serum sensitivity was accompanied by loss of ability to produce extractable K1 antigen and a reduction in the amount of a 46k envelope polypeptide. C-lim cells of strain LP729 exhibited a delayed sensitive response to serum, the degree of lag in serum killing becoming less pronounced with increasing values of D; Mg-lim cells were more sensitive with little or no lag in serum killing. The degree of lag appeared to be directly related to the amount of the 0 side-chain sugar mannose associated with the lipopolysaccharide. C-lim and Mg-lim cultures of E. coli strain LP1395 were resistant to serum except when growing at near maximal rates. Although C-lim cultures contained more acidic polysaccharide than Mg-lim cells, transition to serum sensitivity did not appear to be related to exopolysaccharide production. Rapidly growing cells of strain LPl395 did, however, have lower lipopolysaccharide 0 side-chain sugar : core-sugar ratios than more slowly growing cells. With all three strains, changes in dilution rate and in the nature of the limiting nutrient were accompanied by changes in envelope protein composition.This study demonstrates that many cell-surface changes occur in response to alterations in the growth environment and some of these may be correlated with changes in sensitivity to serum.

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The effect of the growth environment on the serum sensitivity of some urinary Escherichia coli strains

Cited by 7 publications

References 7 publications

Induction of complement sensitivity in Escherichia coli by citric acid and low pH

Induction of complement sensitivity in Escherichia coli by citric acid and low pH

Resistance of Pseudomonas pseudomallei to Normal Human Serum Bactericidal Action

Effect of the growth environment on cell-envelope components of Escherichia coli in relation to sensitivity to human serum

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