The purpose of the study was to determine whether estimated speaking fundamental frequency (ESF0) using the voice and speech range profiles and differences (ΔSF0VRP, ΔSF0SRP, ΔSF0SUM) between the ESF0 and the SF0 measured by electroglottography (SF0EGG) differ according to the presence of voice disorders and gender. Moreover, correlation between SF0EGG and ESF0 measures, and between the G of the GRBAS scale and ΔSF0 measures was explored. Clinical usefulness of ΔSF0 as a screening tool was also explored. Methods: A total of 85 patients with voice disorders and 85 normal adults participated in the study. For each participant, SF0EGG was measured using a passage-reading task and electroglottography. Maximum/minimum pitch, pitch range in Hz and semitone were measured using the VRP and SRP tasks. ESF0VRP, ESF0SRP, ΔSF0VRP, ΔSF0SRP, and ΔSF0SUM were calculated. Results: Significant difference between groups in the ESF0SRP was found only for males. Patient group's ΔSF0 measures were higher than the normal group. Strong positive correlation between the SF0EGG and the ESF0 measures was found. There was weak positive correlation between the G and ΔSF0 measures. The AUC of the ΔSF0VRP, ΔSF0SRP, ΔSF0SUM was .690, .673, .771, the cutoff score 10.867, 10.347, 17.777, respectively. The AUC of the ΔSF0SUM was higher than those of the ΔSF0VRP and ΔSF0SRP. Conclusion: Results indicated that the ΔSF0 showed potential as an objective index for screening voice disorders, especially for those characterized by pitch change. The ΔSF0 could enhance the clinical usefulness of the VRP and SRP.