The effect of the nucleotides immediately upstream of the AUG start codon on the efficiency of translation initiation in sperm cells

Blue light irradiation induces pollen tube rupture in various flowering plants

“…To observe these events under a microscope, the development of a new technique for controlling pollen tube discharge timing and position was eagerly anticipated. Several methods exist for isolating sperm cells from pollen tubes, such as physically cutting pollen tubes with a surgical blade and treatment with different concentrations of mannitol (osmotic shock), ROS, and RALF34 (Sprunck et al, 2012; Shi et al, 2022; Duan et al, 2014; Ge et al, 2017). However, these often result in sample drifting during dissection or chemical treatments, hindering continuous imaging.…”