The effect of the prostaglandin F compounds on the contractility of the pregnant human uterus

Effect of Intravenous Administration of Prostaglandin E1 and F2α on the Contractility of the Non‐Pregnant Human Uterus in Vivo

“…The isolated pure prostaglandin compounds may be administered intravenously by single injections. Such injections given to pregnant patients under in vivo conditions result in an immediate stimulatory response which is reproducible, dose related and easy to evaluate quantitatively (3,22).…”

Prostaglandins

“…However, a selective action of PGE2 and PGFza was observed, since their stimulatory effect was greater on the upper uterine segment compared to the lower segment. of PGF2,, was approximately eight times greater than PGEl with single intravenous injections (244). PGFl, was not as active as PGF2,, and the threshold dose (-100 mcg.)…”

“…Using pregnant human myometrium, Embrey and Morrison (241) reported that both PGEz and PGFz, stimulated myometrial contractility in vitro. In vivo administration of PGEI, PGE2, PGFl,, and PGF2, had a stimulatory effect on the uterus (242)(243)(244)(245). In vivo administration of PGEI, PGE2, PGFl,, and PGF2, had a stimulatory effect on the uterus (242)(243)(244)(245).…”

Structural and Mutational Analysis of Trypanosoma brucei Prostaglandin H2 Reductase Provides Insight into the Catalytic Mechanism of Aldo-ketoreductases

“…PGH 2 chains ␣ and extend in opposite directions and straddle the lip of the crevice. This orientation is justified by the analysis of the electrostatic potential surface model of PGH 2 , which revealed that ␣-chain of the substrate would interact with the only basic residue, Lys 24 , and that -chain may interact with the hydrophobic residue Trp 23 . The hydroxyl group in the -chain of PGH 2 is located at a distance of 2.72 Å from the N ⑀ atom of Trp 23 , suggesting that Trp 23 may well recognize the -chain as evidenced in the TbPGFS⅐NADP ϩ ⅐citrate ternary structure, where the carboxyl O 5 of citrate was hydrogen-bonded to the N ⑀ atom of Trp 23 at a distance of 3.44 Å (Fig.…”

“…15 and 16 for AKR superfamily classification), catalyze the reduction of PGH 2 to PGF 2␣ and PGD 2 to 9␣,11␤-PGF 2 (a stereoisomer of PGF 2␣ ) (17), in addition to the oxidation of 9␣,11␤-PGF 2 to PGD 2 (18,19). In mammals, PGF 2␣ is a potent mediator of various physiological processes (20 -22) including regulation of vascular tone, constriction of uterine muscle (23) and pulmonary arter-ies (24,25), and induction of luteolysis during the estrous cycle and prior to parturition (26,27). During pathological processes in mammals, PGF 2␣ overproduction causes ovarian dysfunction and miscarriage (28 -30).…”