The effect of the shape of mesoporous silica nanoparticles on cellular uptake and cell function

Huang, Xinglu; Teng, Xu; Chen, Dong; Tang, Fangqiong; He, Junqi

doi:10.1016/j.biomaterials.2009.09.060

Cited by 887 publications

(697 citation statements)

References 33 publications

Supporting

Mentioning

660

Contrasting

Unclassified

Order By: Relevance

“…Similar results were obtained in a study where larger amounts of higher aspect ratio rod-shaped mesoporous silica particles were internalized in a fast way by A375 human melanoma cells. [ 103 ] F-actin proteins of the cells that internalized the particles with high aspect ratio appeared disrupted and disorganized. In addition, the F-actin network was anisotropic with poorly formed fi lament bundles accumulated in cell membrane and at the edges of the lamellopodium and fi lopodium.…”

Section: Effect On Internalization and Traffi Ckingmentioning

confidence: 99%

Design Advances in Particulate Systems for Biomedical Applications

Lima

Álvarez-Lorenzo

Mano

2016

Adv Healthcare Materials

View full text Add to dashboard Cite

Particulate systems have been widely used as containers of drugs or cells with the purpose of releasing them in a particularThe search for more effi cient therapeutic strategies and diagnosis tools is a continuous challenge. Advances in understanding the biological mechanisms behind diseases and tissues regeneration have widened the fi eld of applications of particulate systems. Particles are no more just protective systems for the encapsulated drugs, but they play an active role in the success of the therapy. Moreover, particles have been explored for innovative purposes as templates for cells growth and as diagnostic tools. Until few years ago the most relevant parameters in particles formulation were the chemistry and the size. Currently, it is known that other physical characteristics can remarkably affect the performance of particulate systems. Particles with non-conventional shapes exhibit advantages due to the increasing circulation time in blood stream, less clearance by the immune system and more effi cient cell internalization and traffi cking. Creation of compartments has been found useful to control drug release, to tune the transport of substances across biological barriers, to supply the target with more than one bioactive agent or even to act as theranostic systems. It is expected that such complex shaped and compartmentalized systems improve the therapeutic outcomes and also the patient's compliance, acting as advanced devices that serve for simultaneous diagnosis and treatment of the disease, combining agents of very different features, at the same time. In this review, we overview and analyse the most recent advances in particle shape and compartmentalization and applications of newly designed particulate systems in the biomedical fi eld.

show abstract

Section: Effect On Internalization and Traffi Ckingmentioning

confidence: 99%

Design Advances in Particulate Systems for Biomedical Applications

Lima

Álvarez-Lorenzo

Mano

2016

Adv Healthcare Materials

View full text Add to dashboard Cite

show abstract

“…Recent advances in fabrication technologies have enabled generation of shape-specific microparticles and nanoparticles (8-12). These particles, inspired by the diverse, evolutionarily conserved shapes of pathogens and cells, are being used to study the role of carrier shape on cellular internalization, in vivo transport, and organ distribution (6,11,(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23).Despite these pioneering studies, there remains a significant knowledge gap in our fundamental understanding of the interplay between nanoscale shape and size on cellular internalization, especially for clinically relevant polymer-based hydrophilic nanoparticles. Most in vivo drug delivery and imaging applications have proposed the use of nanoparticles with hydrophilic "stealth" surfaces [often achieved through poly(ethylene glycol) (PEG)-based surface modifications] as well as neutral to anionic surface charge, primarily to allow longer in vivo circulation time by reducing protein adsorption and rapid clearance by the reticuloendothelial system (2, 24-27).…”

mentioning

confidence: 99%

mentioning

confidence: 99%

Mammalian cells preferentially internalize hydrogel nanodiscs over nanorods and use shape-specific uptake mechanisms

Agarwal¹,

Singh

Jurney

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

376

336

View full text Add to dashboard Cite

Size, surface charge, and material compositions are known to influence cell uptake of nanoparticles. However, the effect of particle geometry, i.e., the interplay between nanoscale shape and size, is less understood. Here we show that when shape is decoupled from volume, charge, and material composition, under typical in vitro conditions, mammalian epithelial and immune cells preferentially internalize disc-shaped, negatively charged hydrophilic nanoparticles of high aspect ratios compared with nanorods and lower aspect-ratio nanodiscs. Endothelial cells also prefer nanodiscs, however those of intermediate aspect ratio. Interestingly, unlike nanospheres, larger-sized hydrogel nanodiscs and nanorods are internalized more efficiently than their smallest counterparts. Kinetics, efficiency, and mechanisms of uptake are all shape-dependent and cell type-specific. Although macropinocytosis is used by both epithelial and endothelial cells, epithelial cells uniquely internalize these nanoparticles using the caveolae-mediated pathway. Human umbilical vein endothelial cells, on the other hand, use clathrin-mediated uptake for all shapes and show significantly higher uptake efficiency compared with epithelial cells. Using results from both upright and inverted cultures, we propose that nanoparticle internalization is a complex manifestation of three shape-and size-dependent parameters: particle surface-to-cell membrane contact area, i.e., particle-cell adhesion, strain energy for membrane deformation, and sedimentation or local particle concentration at the cell membrane. These studies provide a fundamental understanding on how nanoparticle uptake in different mammalian cells is influenced by the nanoscale geometry and is critical for designing improved nanocarriers and predicting nanomaterial toxicity.cell-uptake mechanism | nanoimprint lithography | drug delivery P olymeric nanoparticles are widely studied for delivering therapeutic and imaging payloads to cells. Understanding how particle properties affect cell uptake is not only critical for designing improved therapeutic and diagnostic agents (1, 2) but also essential for efficient in vitro cell manipulation (3) and evaluating nanomaterial toxicity (4). Nanoparticle uptake by cells has been shown to depend on particle size, surface charge, and material compositions (5-7). Recent advances in fabrication technologies have enabled generation of shape-specific microparticles and nanoparticles (8-12). These particles, inspired by the diverse, evolutionarily conserved shapes of pathogens and cells, are being used to study the role of carrier shape on cellular internalization, in vivo transport, and organ distribution (6,11,(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23).Despite these pioneering studies, there remains a significant knowledge gap in our fundamental understanding of the interplay between nanoscale shape and size on cellular internalization, especially for clinically relevant polymer-based hydrophilic nanoparticles. Most in vivo drug delivery and imaging applicati...

show abstract

“…Esta propriedade é particularmente reforçada por moléculas de PEG de alto peso molecular e altamente ramificado, resultando em comportamento farmacocinético melhorado, circulação sanguínea com meia-vida prolongada e redução da captura pelo sistema reticuloendotelial (BOTTINI et al, 2011;HUANG et al, 2010). Por exemplo, PEG2000 resulta em meia vida de circulação sanguínea de 1,2 h, enquanto PEG6000 aumenta a meia-vida a 5 h; Além disso, o uso de PEG ramificado melhora a meiavida da circulação sanguínea até 15 h (YANG et al, 2012).…”

Section: Superóxido Dismutase (Sod)unclassified

Stress oxidativo e alterações enzimáticas induzidas por nanotubos de carbono de paredes múltiplas (MWCNTs) funcionalizados com polietileno glicol no tecido hepático de camundongos

Irazusta¹,

Oliveira²,

Ceragioli³

et al. 2018

REVINTER

View full text Add to dashboard Cite

Avaliou-se a toxicidade de dois nanotubos de carbono de paredes múltiplas (MWCNT) funcionalizados com PEG, NT1-PEG e NTC-PEG, em fígado de camundongos, 72 h após administração e.v, a 100, 150 e 200 μg / animal. Procedeu-se análise histológica, morfometria da veia centrilobular, atividade antioxidante da SOD e da CAT. O tratamento com MWCNTs-PEG não apresentou diferença clínica comparado ao controle e nenhum processo inflamatório. NT1-PEG produziu alterações mais intensas em enzimas hepáticas que o NTC-PEG, além de causar aumento do diâmetro da veia centrilobular. A administração de MWCNTs induziu toxicidade leve e apenas com 200 μg, provavelmente pela produção de ROS

show abstract

The effect of the shape of mesoporous silica nanoparticles on cellular uptake and cell function

Cited by 887 publications

References 33 publications

Design Advances in Particulate Systems for Biomedical Applications

Design Advances in Particulate Systems for Biomedical Applications

Mammalian cells preferentially internalize hydrogel nanodiscs over nanorods and use shape-specific uptake mechanisms

Stress oxidativo e alterações enzimáticas induzidas por nanotubos de carbono de paredes múltiplas (MWCNTs) funcionalizados com polietileno glicol no tecido hepático de camundongos

Contact Info

Product

Resources

About