The effect of the sub-minimal inhibitory concentration and the concentrations within resistant mutation window of ciprofloxacin on MIC, swimming motility and biofilm formation of Pseudomonas aeruginosa

Shi, Nan; Gao, Yating; Yin, Donghong; Song, Yan; Kang, Jianbang; Li, Xiaoxia; Zhang, Zhiqi; Feng, Xinyuan; Duan, Jinju

doi:10.1016/j.micpath.2019.103765

Cited by 31 publications

(21 citation statements)

References 22 publications

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“…Thus, it is of the greatest importance to find new compounds with inhibitory effects on biofilm formation. Biofilm formation could be promoted by antibiotics under or at MIC, as bacterial defensive strategy [26,27]. In this study, E2-4 and its fractions proved to progressively inhibit biofilm formation of S. aureus, even at low concentrations.…”

Section: Discussionmentioning

confidence: 61%

Abundant Extractable Metabolites from Temperate Tree Barks: The Specific Antimicrobial Activity of Prunus Avium Extracts

et al. 2020

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Tree barks are mainly considered as wood wastes from forestry activities, but represent valuable resources as they may contain antimicrobial compounds. Here, we aimed to evaluate the possible antimicrobial activities of bark extracts and to characterize the chemical composition of the most active extract. Ten methanol bark extracts were tested in vitro against 17 bacterial strains and 5 yeast strains, through minimum inhibitory concentration (MIC) and minimum bactericidal (or fungicidal) concentration (MBC/MFC) assays. The extract from Prunus avium (E2-4) displayed the largest bactericidal activity against Gram-positive bacteria, with a lethal effect on 6 out of 8 strains. Antibiofilm assays of E2-4 were performed by crystal violet staining and enumeration of adhered bacteria. Assays demonstrated a biofilm inhibitory effect of E2-4 against Staphylococcus aureus CIP 53.154 at concentrations equal to or higher than 250 µg/mL. Chemical profiling of E2-4 by 13 C nuclear magnetic resonance (NMR) revealed the presence of dihydrowogonin as a major constituent of the extract. E2-4 was fractionated by centrifugal partition chromatography and the three fractions containing dihydrowogonin were tested for their antibacterial and antibiofilm activities, revealing similar activities to those of E2-4. Dihydrowogonin was positively assessed as an interesting antimicrobial compound, which could be valued from wastes of Prunus avium barks.

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Section: Discussionmentioning

confidence: 61%

Abundant Extractable Metabolites from Temperate Tree Barks: The Specific Antimicrobial Activity of Prunus Avium Extracts

et al. 2020

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“…Rodriguez-Bano et al have illustrated that biofilm-forming A. baumannii isolates were more sensitive to ciprofloxacin and imipenem, 37 suggesting that these strains were not as dependent on antimicrobial resistance as non-biofilm-forming isolates for survival, which was consistent with our result. Moreover, a research has found that the biofilm-forming ability of ciprofloxacin-sensitive Pseudomonas aeruginosa was significantly promoted after exposure to ciprofloxacin, 38 highlighting the importance of biofilms for the survival of sensitive bacteria. Therefore, it seems that the biofilm formation is another survival mechanism besides antimicrobial resistance.…”

Section: Discussionmentioning

confidence: 99%

Phenotypic and Genotypic Characteristics of Biofilm Formation in Clinical Isolates of Acinetobacter baumannii

Li¹,

Ding²,

Liu³

et al. 2021

IDR

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Background: Acinetobacter baumannii is an important pathogen in clinical infections, and biofilm formation is an effective way for A. baumannii to survive under external pressures. In this study, the aims were to examine the antimicrobial resistance, biofilm formation, and biofilm-specific resistance in clinical isolates of A. baumannii. Materials and Methods: A total of 104 clinical A. baumannii isolates were collected from a large teaching hospital in Southwest China. The antibiotics susceptibilities were tested, and biofilm-forming ability was evaluated by crystal violet staining by confocal laser scanning microscopy (CLSM). Minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), minimum biofilm inhibitory concentration (MBIC), and minimum biofilm eradication concentration (MBEC) of ciprofloxacin, meropenem, and ceftazidime were tested on selected strains by broth microdilution method. Biofilm-associated genes were detected by polymerase chain reaction (PCR), and expression of genes at planktonic stage and biofilm stage were analyzed by real-time reverse transcription PCR (RT-PCR). Results: Multidrug-resistant (MDR) isolates accounted for 65.4%, but no strain was resistant to tigecycline and polymyxin B. Moreover, non-MDR strains tended to form stronger biofilms than MDR strains, and a negative correlation between biofilm-forming ability and resistance profiles to each of tested antimicrobials were observed. The MBECs and MBICs of ciprofloxacin, ceftazidime, and meropenem were evidently increased compared with MICs and MBCs among all tested strains. Additionally, the biofilm formation ability of the csuDpositive strains was stronger than that of the csuD-negative strains. The strains in MDR group had higher carrying rate of csuA and csuD genes than non-MDR group, while non-MDR strains possessed more ompA gene than MDR group. Finally, abaI gene was significantly up-regulated after biofilm formation. Conclusion:These results revealed valuable data for the negative correlation between antimicrobial resistance and biofilm formation, as well as phenotypic and genotypic characteristics of biofilm formation in A. baumannii.

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“…Subinhibitory levels of arylomycin have been shown to strongly inhibit both swimming and swarming motility in E. coli, thereby impeding biofilm formation (Walsh et al 2019). Interestingly, studies have also reported antibiotic sub-MICs to enhance bacterial motility (Shi et al 2019). Discrete findings have also pinpointed the inhibition of bacterial motility via attenuation of key virulence elements (Gupta et al 2016;Walsh et al 2019).…”

Section: Sub-mic Of Na Retards Bacterial Motilitymentioning

confidence: 99%

Subinhibitory concentrations of nalidixic acid alter bacterial physiology and induce anthropogenic resistance in a commensal strain of Escherichia coli in vitro

Chadha

Khullar

2021

Lett Appl Microbiol

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Significance and Impact of the Study:The effect of sublethal concentrations of antibiotics on commensal micro-organisms is less studied compared to that of pathogenic micro-organisms. For the first time, the influence of nalidixic acid (NA) sub-MICs on a commensal strain of E. coli was reported. The findings precisely demarcate the role of NA sub-MICs in altering bacterial growth rate and physiology and inducing genomic aberrations, thus resulting in the emergence of antibiotic resistance. This justifies the implications of sublethal concentrations of antibiotics as signalling molecules that drive microbial pathogenicity and disseminate antimicrobial resistance (AMR).

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The effect of the sub-minimal inhibitory concentration and the concentrations within resistant mutation window of ciprofloxacin on MIC, swimming motility and biofilm formation of Pseudomonas aeruginosa

Cited by 31 publications

References 22 publications

Abundant Extractable Metabolites from Temperate Tree Barks: The Specific Antimicrobial Activity of Prunus Avium Extracts

Abundant Extractable Metabolites from Temperate Tree Barks: The Specific Antimicrobial Activity of Prunus Avium Extracts

Phenotypic and Genotypic Characteristics of Biofilm Formation in Clinical Isolates of Acinetobacter baumannii

Subinhibitory concentrations of nalidixic acid alter bacterial physiology and induce anthropogenic resistance in a commensal strain of Escherichia coli in vitro

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