The effect of thick fibers and large pores of electrospun poly(ε-caprolactone) vascular grafts on macrophage polarization and arterial regeneration

Wang, Zhihong; Cui, Yun; Wang, Jianbo; Yang, Xiaohu; Wu, Yifan; Wang, Kai; Gao, Xuan; Liu, Dong; Li, Yuejie; Zheng, Xi-Long; Zhu, Yan; Kong, Deling; Zhao, Qiang

doi:10.1016/j.biomaterials.2014.03.078

Cited by 383 publications

(290 citation statements)

References 39 publications

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“…Fibre diameter has shown to affect the gene expression of cells (Hodgkinson et al 2014;Wang et al 2014;Bean & Tuan 2015), and cryogenic scaffolds have shown to effect the behaviour of scaffolds and give a better representation on an in vivo environment for drug development (Bulysheva et al 2013). We found that small diameter fibres at 7 days had a significant reduction in KIM-1 expression compared to tissue culture plastic in both aligned and cryogenic scaffolds, possibly indicating healthier cells (Waanders et al 2010).…”

Section: Discussionmentioning

confidence: 99%

The effect of electrospun polycaprolactone scaffold morphology on human kidney epithelial cells

2017

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There is a pressing need for further advancement in tissue engineering of functional organs with a view to providing a more clinically relevant model for drug development and reduce the dependence on organ donation. Polymer based scaffolds, such as polycaprolactone (PCL), have been highlighted as a potential avenue for tissue engineered kidneys, but there is little investigation down this stream.Focus within kidney tissue engineering has been on 2-dimensional cell culture and decellularised tissue. Electrospun polymer scaffolds can be created with a variety of fibre diameters and have shown a great potential in many areas. The variation in morphology of tissue engineering scaffold has been shown to effect the way cells behave and integrate. In this study we examined the cellular response to scaffold architecture of novel electrospun scaffold for kidney tissue engineering. Fibre diameters of 1.10±0.16 µm and 4.49±0.47 µm were used with 3 distinct scaffold architectures. Traditional random fibres were spun onto a mandrel rotating at 250 rpm, aligned at 1800 rpm with novel cryogenic fibres spun onto a mandrel loaded with dry ice rotating at 250 rpm. Human kidney epithelial cells were grown for 1 and 2 weeks. Fibre morphology had no effect of cell viability in scaffolds with a large fibre diameter but significant differences were seen in smaller fibres. Fibre diameter had a significant effect in aligned and cryogenic scaffold. Imaging detailed the differences in cell attachment due to scaffold differences. These results show that architecture of the scaffold has a profound effect on kidney cells; whether that is effects of fibre diameter on the cell attachment and viability or the effect of fibre arrangement on the distribution of cells and their alignment with fibres. Results demonstrate that PCL scaffolds have the capability to maintain kidney cells life and should be investigated further as a potential scaffold in kidney tissue engineering.

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Section: Discussionmentioning

confidence: 99%

The effect of electrospun polycaprolactone scaffold morphology on human kidney epithelial cells

2017

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“…For instance, He et al fabricated a bi-layered elastomeric porous vascular scaffolds by thermally induced phase separation method and the VSMCs could infiltrate into the wall of the grafts in vivo [13.] Wu et al reported that a porous tubular poly (glycerol-sebacate) scaffolds fabricated by salt leaching method could realize sufficient cell infiltration in vivo [14]. In our previous study, the electrospun poly (ε-caprolactone) (PCL) vascular grafts with thick fibers and large pore obviously enhanced cell infiltration into the grafts wall compared with the grafts of thin fibers [16]. Despite some encouraging results have been obtained, the regenerated VSMCs in these works arranged randomly due to the lack of well-defined aligned geometric cues.…”

Section: Introductionmentioning

confidence: 93%

“…Scaffold porosity, fiber and pore size measurements Scaffold porosity was measured by a liquid intrusion method [16]. Dry grafts were weighed and then immersed in 100% ethanol for 2 h. Grafts were then gently wiped to remove excess ethanol and weighed again.…”

Section: Mechanical Testsmentioning

confidence: 99%

“…For this objective, suitable pore size and highly interconnected porous structure of the scaffolds are essentially important [11,12]. Up to date, the vascular scaffolds with different pore structure have been developed by various techniques such as phase separation [13], salt leaching [14], electrospinning [15,16] and so on. For instance, He et al fabricated a bi-layered elastomeric porous vascular scaffolds by thermally induced phase separation method and the VSMCs could infiltrate into the wall of the grafts in vivo [13.] Wu et al reported that a porous tubular poly (glycerol-sebacate) scaffolds fabricated by salt leaching method could realize sufficient cell infiltration in vivo [14].…”

Section: Introductionmentioning

confidence: 99%

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Circumferentially aligned fibers guided functional neoartery regeneration in vivo

Zhu¹,

Wang²,

Zhang³

et al. 2015

Biomaterials

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100

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“…In a progressive study, Wang et al (2014) examined the effect of PCL fiber diameter and pore size on arterial regeneration in a rat abdominal aorta model. All grafts remained patent through www.frontiersin.org 28 days, with one thicker-fiber (5-6 µm) graft becoming occluded at 100 days (80% graft patency).…”

Section: Tissue Engineering Of Vascular Grafts In Vivomentioning

confidence: 99%

Vascular Grafting Strategies in Coronary Intervention

2014

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With the growing need for coronary revascularizations globally, several strategies to restore blood flow to the heart have been explored. Bypassing the atherosclerotic coronary arteries with autologous grafts, synthetic prostheses, and tissue-engineered vascular grafts continue to be evaluated in search of a readily available vascular graft with clinically acceptable outcomes. The development of such a vascular graft including tissue engineering approaches both in situ and in vitro is herein reviewed, facilitating a detailed comparison on the role of seeded cells in vascular graft patency.

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The effect of thick fibers and large pores of electrospun poly(ε-caprolactone) vascular grafts on macrophage polarization and arterial regeneration

Cited by 383 publications

References 39 publications

The effect of electrospun polycaprolactone scaffold morphology on human kidney epithelial cells

The effect of electrospun polycaprolactone scaffold morphology on human kidney epithelial cells

Circumferentially aligned fibers guided functional neoartery regeneration in vivo

Vascular Grafting Strategies in Coronary Intervention

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