The effect of thiolated phospholipids on formation of supported lipid bilayers on gold substrates investigated by surface-sensitive methods

Fluorescence-Amplified Detection of Redox Turnovers in Supported Lipid Bilayers Illuminates Redox Processes of α-Tocopherol

“…Rapid recovery of the fluorescence intensity revealed the formation of a well-connected two-dimensional solid-SLB when the SLB was formed upon temperature increase in a stepwise fashion from 23 °C to 27 °C, 34 °C, and finally 37 °C (Figure ). Notably, a lack of DiD fluorescence recovery at 23 °C and 27 °C was observed, suggesting the formation of a layer consisting of unruptured liposomes , (Figure a,b, green and purple traces), and a “phase transition” to an SLB occurred only upon further heating to 34 and 37 °C (Figure a,b, red and blue traces, respectively). Attempts at forming an SLB on ITO-coated surfaces directly at 37 °C were unsuccessful.…”

Cell-membrane-inspired polymers for constructing biointerfaces with efficient molecular recognition

“…To stably organize phospholipid molecules on a gold substrate, phosphatidylcholine derivatives have been synthesized by introducing thiol or sulfide bonds at the ends of the hydrophobic moieties. 116,117 Gold substrates are used primarily for bioanalysis devices, such as quartz crystal microbalances (QCMs), surface plasmon resonators (SPRs), and other electrodes. 118,119 Phospholipid molecules form a spontaneously organized structure on a gold surface as a monolayer.…”

Thiolated Nanoparticles for Biomedical Applications: Mimicking the Workhorses of Our Body

“…Shen et al designed a cysteine-polyethylene glycol stearate conjugate as illustrated in Table 2 and prepared NLC with this thiolated excipient by a fusion-emulsification method. [50] As thiol groups might be oxidized during the preparation process, however, either a pH < 5 is needed in order to keep the www.advancedsciencenews.com www.advancedscience.com S-Protected thiolated palmitic acid Nanostructured lipid carriers [52] Cysteine-polyethylene glycol stearate Nanostructured lipid carriers [50][51][52][53][54][55][56][57][58][59] N-Acetyl-l-cysteine-polyethylene glycol stearate Nanostructured lipid carriers [60] Thioglycolic acid-octylamine conjugate SEDDS [61] Cysteine-functionalized phospholipid (Cys-PEG-DSPE)…”

“…Liposomes [62] Cholesterol (Chol)-integrated tetraether lipid comprising of a cleavable disulfide bond Liposomes [63] 1,2-Dipalmitoyl-sn-glycero-3phosphothioethanol Liposomes [51] Multifunctional thiolated amino lipid Lipid-based siRNA delivery systems [64] Lipoic acid derivative Liposomes [54] (Continued) Lipoic acid derivative Liposomes [53] Lipoic acid derivatives Lipophilic complexes with DNA [55] Table 3. Lipophilic NPs coated with a thiolated polymer.…”

“…An advantage of lipid‐based thiolated NPs lies in the great flexibility for the design of such systems, as numerous surfactants and lipophilic excipients can be combined on purpose resulting in NPs of very different properties. Independently from the applied preparation method such as melt‐emulsification method for solid lipid nanoparticles and nanostructured lipid carriers (NLC) [ 50 ] or thin‐film hydration and extrusion method for unilamellar liposomes [ 51 ] this process works for all types of lipid based NPs in the same way. Consequently, there are no different or additional preparation techniques needed.…”